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Method for preparing indissoluble aquatic collagen protein source ACE inhibitor peptides

A technology of aquatic collagen and collagen, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of complex process, low product efficiency, high production cost, etc., and achieve simple production process, increase productivity, and production low cost effect

Inactive Publication Date: 2009-06-17
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Marine collagen generally has the characteristics of fine fiber structure, and the fiber structure is easily damaged during the extraction process, which affects the quality and extraction rate of high-molecular collagen, resulting in low product efficiency
However, the method of increasing the extraction temperature or prolonging the enzymatic hydrolysis time can improve the extraction rate of high molecular collagen, but it is very easy to cause the decomposition of high molecular collagen, and it is difficult to obtain ideal high molecular collagen.
[0004] At present, under low temperature conditions, acidic solutions such as acetic acid and citric acid containing pepsin are used to slowly decompose marine organisms to extract high-molecular collagen, but this method will eventually leave a large amount of high-value collagen. In the production residue, the productivity of high molecular collagen is extremely low, and the process is complicated and the production cost is high

Method used

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  • Method for preparing indissoluble aquatic collagen protein source ACE inhibitor peptides
  • Method for preparing indissoluble aquatic collagen protein source ACE inhibitor peptides

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Take 100g of cleaned fresh scallop mantle, cut it into 1cm×1cm pieces at a temperature of 4°C, put the cut pieces into 500ml distilled water, and grind them into a pulp at a temperature of 4°C. And put into the high-speed centrifuge with the rotating speed of 10000rpm high-speed centrifugation 10min to remove the impurities such as part fat and pigment, obtain precipitate; Take the caustic soda solution that the equivalent concentration is 0.1N, the precipitate obtained is added in this caustic soda solution, Control the mass ratio of caustic soda solution to sediment to 20:1, place it in an environment with a temperature of 4°C, stir for 12 hours, then centrifuge at a speed of 5000rpm to remove the supernatant; wash the precipitate with distilled water 3 times, After each cleaning, remove the upper cleaning solution by high-speed centrifugation to obtain the sediment raw material; respectively take acetic acid solution and pepsin (pig stomach source, EC 3.4.23.1) with a...

Embodiment 2

[0042] Take 100g of cleaned fresh scallop mantle, cut into 1cm×1cm pieces at a temperature of 2°C, put the cut pieces into 800ml distilled water, and grind them into pulp at a temperature of 2°C. And put into the high-speed centrifuge with the rotating speed of 5000rpm high-speed centrifugation 30min to remove impurities such as part of fat and pigment, obtain precipitate; Get the caustic soda solution that the equivalent concentration is 0.1N, the precipitate obtained is added in this caustic soda solution, Control the mass ratio of the caustic soda solution to the precipitate to be 15:1, place it in an environment with a temperature of 2°C, stir for 48 hours, then centrifuge at a speed of 10,000 rpm to remove the supernatant; wash the precipitate with distilled water for 3 times, After each cleaning, remove the upper cleaning solution by high-speed centrifugation to obtain the raw material of the precipitate; respectively take citric acid solution and pepsin (pig stomach sour...

Embodiment 3

[0044] Get cleaned fresh scallop mantle 100g, under the condition of 3 DEG C, cut into pieces of 1cm * 1cm, put the cut pieces into 650ml distilled water, under the condition of 3 DEG C, mince them into pulp, And put into the high-speed centrifuge with the rotating speed of 7500rpm high-speed centrifugation 20min to remove impurities such as part of fat and pigment, obtain precipitate; Get equivalent concentration and be the caustic soda solution of 0.1N, add the precipitate obtained in this caustic soda solution, Control the mass ratio of the caustic soda solution to the precipitate to be 18:1, place it in an environment with a temperature of 3°C, stir for 30 hours, then centrifuge at a speed of 7500rpm to remove the supernatant; wash the precipitate with distilled water 3 times, After each cleaning, the upper cleaning solution was removed by high-speed centrifugation to obtain the sediment raw material; the oxalic acid solution and acid protease (Aspergillus niger) with a mol...

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Abstract

The invention discloses a preparation method of a less soluble aquatic collagen source ACE inhibitory peptide comprising: cutting and crushing fresh marine organism at low temperature, adding a caustic soda to stir at low temperature, removing a supernatant, cleaning precipitates and adding an organic acid solution and an acid protease, stirring at low temperature, obtaining the high molecule collagen and less soluble collagen through speed centrifugation, enabling the less soluble collagen denaturation at high temperature and high pressure, forming a soluble collagen solution, obtaining soluble collagen powder; adding a sodium hydrate solution and the protease to stir at optimal temperature, obtaining an enzymolysis protein peptide solution, centrifugally separating said solution, collecting the supernatant, preparing the less soluble aquatic collagen source ACE inhibitory peptide after the freeze drying. The method of the invention fully use the dregs after the high molecule collagen extraction, obtains the hot water soluble collagen, and obtains the collagen peptide through enzymolysis, prepares eatable ACE inhibitory peptide products and improves the high molecule collagen productivity.

Description

technical field [0001] The invention relates to a preparation method of an ACE inhibitory peptide, in particular to a preparation method of an insoluble aquatic collagen source ACE inhibitory peptide. Background technique [0002] Hypertension, a type of lifestyle-related disease, is a common disease in modern society and causes various diseases. The existing commonly used drugs for the treatment of hypertension are captopril and enalapril, which are chemically synthesized and have the effect of inhibiting angiotensin-converting enzyme. These medicines have side effects in varying degrees, and long-term use is not conducive to the health of patients. Therefore, from the perspective of safety, the development of antihypertensive protein peptides that use natural food resources as raw materials has become the research focus of many scholars. In recent years, some results have been obtained in extracting active ingredients for treating hypertension from soybean protein, garli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14
Inventor 申铉日易美华李从发
Owner HAINAN UNIVERSITY
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