Method for extracting and preparing DHA (docosahexaenoic acid) of algae oil

An algal oil and solvent extraction technology, which is applied in the direction of fat oil/fat refining, fat oil/fat production, and fat production, can solve the problems of low yield, backward preparation technology, low production efficiency, etc. amount of effect

Inactive Publication Date: 2016-06-15
SHENZHEN VIGOR HEALTH CARE PROD CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] At present, the existing preparation technology for extracting DHA from microalgae is relatively backward, with lo

Method used

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  • Method for extracting and preparing DHA (docosahexaenoic acid) of algae oil

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preparation example Construction

[0062] A method for extracting and preparing algae oil DHA disclosed by the invention comprises the following steps:

[0063] (1) cultivating and fermenting Cryptidium koushii; the culturing and fermenting are algae culturing and heterotrophic fermentation to obtain microalgae raw materials;

[0064] (2) drying; the microalgae raw material obtained in step (1) is dried to obtain microalgae powder;

[0065] (3) wall-breaking pretreatment; the microalgae powder obtained in step (2) is subjected to microalgae wall-breaking pretreatment by ultrasonic treatment to obtain microalgae dry powder;

[0066] (4) Microalgae DHA extraction; the microalgae dry powder obtained in step (3) is extracted by Soxhlet extraction method and / or supercritical carbon dioxide extraction method to obtain microalgae oil;

[0067] (5) urea inclusion enrichment and purification; the microalgae oil obtained in step (4) is enriched and purified by urea inclusion to purify DHA; obtain microalgae DHA algae oi...

specific Embodiment

[0102] In some embodiments, we have carried out specific examples of fermentation conditions, wherein temperature and pH values ​​are controlled differently to obtain more preferred technical solution effects; specific examples are as follows:

[0103] (1) Embodiment about temperature:

[0104] The optimum temperature for microalgae growth and oil accumulation varies from species to species. After the secondary seeds are inserted into the fermentation medium, they are fermented at temperatures of 15°C, 20°C, 25°C, 30°C, and 35°C. The effects of different temperatures on the biomass and oil content of Cryptodinium algae were studied.

[0105] (2) Embodiment about pH value:

[0106] The initial pH value of the medium is one of the important factors affecting the growth rate of microalgae. Many researchers have found that various algae grow fastest around pH5-7, and it is difficult to grow under extremely acidic or alkaline conditions. The initial pH of the fermentation medium...

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Abstract

The invention discloses a method for extracting and preparing algae oil DHA, which comprises the following steps: (1) cultivating and fermenting Cryptidium koii; (2) drying; (3) pretreatment by breaking the wall; (4) microalgae DHA extraction; (5) urea inclusion enrichment and purification; the microalgae oil obtained in step (4) is enriched and purified by urea inclusion method to purify DHA; obtain microalgae DHA algae oil; (6) decolorization and deodorization; urea inclusion The microalgal DHA algae oil obtained by enrichment and purification is decolorized by adding activated clay accounting for 3% of the oil body mass. The decolorization temperature is 80°C and the decolorization time is 45 minutes. Steam stripping deodorization, the stripping temperature is 85°C, the vacuum degree is 0.30Kpa, the deodorization time is 1 hour, then slowly cool under the vacuum state, and centrifuge to obtain the algae oil DHA product.

Description

technical field [0001] The invention relates to a method for extracting DHA from algae oil, in particular to extracting DHA oil from microalgae. Background technique [0002] The important physiological functions of DHA in the central nervous system, cardiovascular system and visual nervous system have been widely recognized by the society. Many countries in the world have stipulated that DHA can be used as a nutritional fortifier and food additive. At present, DHA has been used in infant food, dairy products, beverages, soybean milk and edible oils, etc., and the market demand is very large. Therefore, the production of DHA contains huge business opportunities. [0003] The main sources of DHA are fish oil and algae oil. Fish oil has a large fishy smell and high content of EPA, and it is difficult to add to food. The number of people who eat it is also limited, and the resources of fish oil are limited. The large-scale, high-density microalgae heterotrophic fermentation c...

Claims

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Application Information

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IPC IPC(8): C11B1/04C11B1/10C11B3/00C11B3/10C11B3/12
CPCC11B1/025C11B1/10C11B1/104C11B3/001
Inventor 于荣贤刘兵余玉芳张勇李建方翠孙小玲孙会喜
Owner SHENZHEN VIGOR HEALTH CARE PROD CO LTD
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