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Methods for preparing oil bodies comprising active ingredients

An oil body and active agent technology, which is applied in the directions of organic active ingredients, medical preparations containing active ingredients, ester active ingredients, etc., can solve the problems of poor distribution of active ingredients and unattainable distribution.

Inactive Publication Date: 2006-11-15
SEMBIOSYS GENETICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some partitioning of the active ingredient can be facilitated by simple mixing according to the aforementioned Deckers patent, frequently no partitioning is achieved or the partitioning of the active ingredient is suboptimal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] The washed oil body preparation was obtained from safflower.

[0120] This example describes the recovery of the oil body fraction from safflower. The resulting preparation contains intact washed oil bodies.

[0121] Seeds decontaminate. A total of 45 kg of dried safflower (Carthamus tinctorius) seeds were washed twice with about 120L of 65°C tap water and once with about 120L of 15°C tap water. Washing takes place in drums with screen mesh to separate waste water.

[0122] Milling of seeds. The washed seeds were dumped through a colloid mill (Colloid Mill, MZ-130 (Fryma); capacity: 350kg / hr) funnel equipped with a MZ-120 cross toothed rotor / stator milling device and a top loading funnel, Provide about 100L 25mM pH7.0 NaH through an externally connected rubber tube before milling 2 PO 4 buffer. The operation of the colloid mill is carried out under 1R gap correction, and particles smaller than 100um are selected at 18°C ​​and 30°C. 45 kg of all seeds were milled...

Embodiment 2

[0126] Partition of clobemethasone dipropionate into washed safflower oil.

[0127] Washed safflower oil bodies were prepared as described in Example 1. Oil bodies were preserved using 0.1% Neolone 950 and 0.1% glycacil L. Weigh clobemethasone propionate (CP) (supplier-Sigma) (12-30mg) into a clean and dry 16×100mm screw-cap heat-resistant glass test tube, mix with 300ul isopropanol, then mix with 200mg safflower oil Mix, vortex combined samples, then incubate at 34°C for 20 minutes. After incubation, samples were vortexed again, then dried under nitrogen for 20 minutes to remove isopropanol. Add 1 ml of washed high dry heavy oil bodies to the CP / safflower oil mixture at room temperature, centrifuge briefly to pellet the contents to mix thoroughly, vortex and further incubate overnight at 34-42°C in a sealed tube to allow CP and safflower oil to blend. into the oil body. Use hexane extraction to determine the amount of CP present in free oil (free oil is defined as oil not...

Embodiment 3

[0129] Distributes tretinoin into body of washed safflower oil.

[0130]Washed safflower oil bodies were prepared as described in Example 1. Oil bodies were preserved using 0.1% Neolone 950 and 0.1% glycacil L. Retinoic acid (RA) (supplier - Sigma) (1-8 mg) was weighed into a clean dry 16 x 100 mm screw cap pyrex test tube and mixed with 3 ml of isopropanol. Safflower oil was added so that it did not exceed 5 mg RA per gram of safflower oil. The combined samples were vortexed and heated in a constant temperature dry bath at 40-45°C. It was then dried under a steady stream of nitrogen to remove the isopropanol (approximately 0.5-1 hour). Then, add 4-5ml of washed high-dry heavy oil body to the RA / safflower oil mixture per gram of safflower oil used to dissolve RA, note: keep the RA / safflower oil mixture in a constant temperature dry bath until the oil is added immediately body before. The oil body mixture was centrifuged briefly to pellet the contents to mix the contents t...

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PUM

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Abstract

The present invention provides novel emulsions that comprise oil bodies. The invention also relates to novel methods for generating formulations comprising oil bodies and active ingredients wherein the active ingredient is partitioned into the oil body. The methods are particularly useful for generating emulsions with either hydrophobic or amphipathic biologically active agents.

Description

technical field [0001] The present invention provides novel emulsions containing oil bodies. The invention also relates to a novel process for the preparation of formulations comprising oil bodies and active agents, wherein the active ingredient is partitioned into the oil bodies. This method is particularly useful for preparing emulsions containing hydrophobic or amphiphilic bioactive agents. Background technique [0002] In the seeds of oilseed crops, including economically important crops such as soybean, rapeseed, sunflower, safflower, and palm, the water-insoluble lipid fraction is stored in separate subcellular structures known in the art such as oil bodies, oil In plastids, liposomes or spheroids (Huang 1992, Ann. Rev. Plant Mol. Biol. 43:177-200). In addition to chemically defined oil mixtures of fatty acid glycerides (triacylglycerols), oil bodies also include phospholipids and a number of related proteins, collectively known as oleosins. From the structural point...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K8/06A61K8/97A61K9/127A61K38/00A61K45/00A61Q19/00C07K5/00C07K7/00
CPCA61K8/06A61K9/107A61K31/24A61K31/685A61K36/286A61Q19/00A61P3/02A61P17/00A61P17/06A61P23/02A61P29/00
Inventor 伊莉莎白·旺达·默里约瑟夫·布斯南希-安·马克里
Owner SEMBIOSYS GENETICS INC
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