Method for improving biosynthesis efficiency of pingyangmycin

A technology of Pingyangmycin and production efficiency, which is applied in the field of microbiology and bioengineering, and can solve the problems of few reports on fermentation technology

Active Publication Date: 2021-01-05
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this field, the research on Pingyangmycin mainly focuses on its clinical application, while the research on its fermentation process is rarely reported.

Method used

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  • Method for improving biosynthesis efficiency of pingyangmycin
  • Method for improving biosynthesis efficiency of pingyangmycin
  • Method for improving biosynthesis efficiency of pingyangmycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1, the impact of different precursor substances on the synthesis of Pingyangmycin

[0077] The biosynthesis of Pingyangmycin requires various precursor substances such as sugars, amino acids and polypeptides. In the previous work, the inventors selected many types of precursor substances for research, and changed various synthetic conditions to understand different precursor substances. The effects of body substances and different conditions on the synthesis of pingyangmycin. After research on a large number of substances and experimental conditions, the inventors delineated the precursor substances that may affect the reaction as amino acid compounds.

[0078] The inventor of the present invention took the fermentation system without adding precursor substances as a comparison, and compared the addition of 0.1% (W / V) serine, asparagine, histidine, alanine, threonine, cysteine, methionine Fermentation of pingyangmycin in the system of compounds such as acid,...

Embodiment 2

[0084] Example 2, Optimal Research on Citrulline Improving the Fermentation Efficiency of Pingyangmycin

[0085] After the inventors have determined the compound that promotes the biosynthesis of Pingyangmycin. During further optimization studies, various fermentation conditions were considered. According to the investigation of multiple factors and multiple conditions, determining the amount and timing of compound addition is a favorable factor for further improving the fermentation efficiency.

[0086] 1. The effect of the amount of citrulline added on the fermentation of Pingyangmycin

[0087] When fermenting for 48 hours, citrulline with different concentrations of 0, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12, and 0.14% (W / V) was added to carry out the shake flask fermentation experiment, and each concentration was set to 3 parallels to take the average data , taking the group without citrulline as the control.

[0088] The result is as figure 2 shown. The results showed th...

Embodiment 3

[0096] Example 3. Optimization research on ornithine improving the fermentation efficiency of Pingyangmycin

[0097] 1. The effect of the amount of ornithine added on the fermentation of Pingyangmycin

[0098] At 48h after the start of fermentation, ornithine with different concentrations of 0, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12, and 0.14% (W / V) was added to carry out the shake flask fermentation experiment, and each concentration was set up with 3 parallel samples. Average data, with the group without ornithine as the control.

[0099] The result is as Figure 4 shown. The results show that when the concentration of ornithine is added at 0.02-0.12% (W / V), the potency is significantly improved, and the effect is ideal in the range of 0.04-0.1% (W / V). Among them, the most ideal is 0.06% (W / V), and the fermentation titer reaches 132.21 μg / mL, which is 68.12% higher than that of the control group (78.64 μg / mL).

[0100] The result shows that when the addition of ornithine is ...

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Abstract

The invention provides a method for improving the biosynthesis efficiency of pingyangmycin. The invention reveals for the first time that a proper amount of specific amino acid is added in a proper stage of fermentation of a pingyangmycin producing strain, so that the synthesis efficiency of pingyangmycin biosynthesis can be remarkably improved, and the yield is greatly increased. According to thetechnical scheme, on the basis that the cost of a fermentation culture medium is not remarkably increased, the biosynthesis yield of the pingyangmycin is remarkably increased; and meanwhile, the shortening of the biosynthesis period of the pingyangmycin becomes possible.

Description

technical field [0001] The invention belongs to the field of microbiology and bioengineering, and more specifically, the invention relates to a method for improving the biosynthesis efficiency of pingyangmycin. Background technique [0002] In a certain growth period, microorganisms use primary metabolites as precursors to synthesize some substances that have no clear function for the life activities of microorganisms. The process is secondary metabolism, and the products of this process are secondary metabolites. According to their physiological functions, they are divided into antibiotics, hormones, alkaloids, pigments, toxins, enzyme inhibitors, etc. [0003] The synthetic process of using microorganisms to synthesize antibiotics can generally be summarized into several steps: precursor polymerization step, structural modification step, and assembly step of different components. Although there are similarities in the synthesis process, there are still many differences in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/625
CPCC12P21/005C12P21/02C07K9/003
Inventor 郭美锦吴涛庄英萍储炬张海涛朱颖
Owner EAST CHINA UNIV OF SCI & TECH
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