A chemical mutagen for genetic breeding of maize

A chemical mutagen, genetic breeding technology, applied in the direction of chemicals, applications, biocides for biological control, etc., can solve the problem that the physiological damage of maize M1 is not obvious, the method and mutagen is single, and the chromosome aberration rate is not too high. Advanced problems, to achieve the effect of improving the efficiency of induced mutation, easy operation, and simple processing methods

Active Publication Date: 2021-12-24
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology

There are few research reports on the treatment of corn seeds with chemical agents, and all of them use EMS-paraffin oil to treat inbred line pollen, and the method and mutagen are relatively simple.
[0004] Pingyangmycin PYM is an antibiotic, and PYM is a new mutagen discovered by Chinese researchers, which is similar to the mutagenic characteristics of EMS; however, previous studies have found that the physiological damage of maize M1 is not obvious when treated with PYM alone. Chromosomal aberration rate is not too high

Method used

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  • A chemical mutagen for genetic breeding of maize
  • A chemical mutagen for genetic breeding of maize
  • A chemical mutagen for genetic breeding of maize

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Embodiment Construction

[0013] Test material: Jingkenuo 2000; purchased from Corn Research Center of Beijing Academy of Agriculture and Forestry Sciences

[0014] PYM (pingyangmycin), EMS (ethyl methylsulfonate), MNU (N-methyl-N-nitrosourea); commercially available.

[0015] Test method: chemical mutagen seed treatment (refer to An Xueli, etc.). Select healthy corn seeds, wash them with clean water, put them into 5% sodium hypochlorite solution for disinfection for 0.5h, pre-soak them with distilled water at room temperature for 24h after disinfection, absorb the surface moisture with absorbent paper, and use the following chemical mutagens at different concentrations Treatment, the ratio of liquid medicine and seed is 1 grain / ml, treat 8h, wash 4h after using tap water, after seed dries, sow in sand basin, observe the germination potential of M1 generation, germination rate, the growth height of three-leaf stage seedling , root length, root number. Note: All mutagens were diluted with 0.06mol / L ph...

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Abstract

The invention provides a kind of chemical mutagen that is used for corn genetic breeding, described chemical mutagen is the combination of Pingyangmycin and N-methyl-N-nitrosourea; Wherein the concentration of Pingyangmycin is 20ppm, The concentration of N-methyl-N-nitrosourea is 0.2%. The chemical mutagen can be used in corn genetic breeding.

Description

technical field [0001] The invention belongs to the field of agricultural genetic breeding, in particular to a chemical mutagen for corn genetic breeding. Background technique [0002] Corn is the third largest grain crop in my country, second only to wheat and rice, and has important economic value. With the further research and utilization of maize heterosis, the requirements for breeding materials are getting higher and higher. Creating breeding materials with rich genetic basis is an urgent problem for breeders to solve. Maize chemical mutation breeding is a modern biological technology that uses chemical mutagens to treat different parts of maize to cause mutations, and to study, screen and identify the mutants. In artificial mutation breeding, it has the advantages of low cost, convenient use, simple operation, and specific mutagenic effect. It can increase the frequency of mutations, create rich types of mutated materials, and effectively solve the problem of poor co...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N41/04A01N47/28A01N43/16A01P21/00A01H1/06
CPCA01N41/04A01N43/16A01N47/28A01H1/06
Inventor 陈曙金辉赵秋芳
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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