Fermentation medium and fermentation method for producing Bleomycin A5

A fermentation medium and Pingyangmycin technology, applied in the field of industrial microorganisms, can solve the problems of high production cost and difficulty in expanding production, and achieve the effect of increasing production and reducing costs

Active Publication Date: 2021-02-23
SHANGHAI INST OF PHARMA IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to overcome defects such as the high production cost of fermenting Pingyangmycin in the prior art, and being difficult to expand production, and to provide a kind of production Pingyangmycin Vegetable fermentation medium and fermentation method

Method used

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  • Fermentation medium and fermentation method for producing Bleomycin A5
  • Fermentation medium and fermentation method for producing Bleomycin A5
  • Fermentation medium and fermentation method for producing Bleomycin A5

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Taking the formula of the basic fermentation medium (the above-mentioned medium three) as a comparison, under the condition that the components such as carbon source and inorganic salt remain unchanged, other nitrogen sources are kept constant, and the amount of corn steep liquor is explored, and the amount of corn steep liquor is changed from 0.75% to 0.75%. Adjust to 1%, shake the bottle to ferment for 8 days, and use high performance liquid phase to measure the fermentation unit.

[0057] Table 1

[0058] medium three Example 1 Amount of corn syrup (%) 0.75 1 Relative potency 1.00 1.04

[0059] The optimized formula is: 1% corn steep liquor, 3.5% cold-pressed soybean powder, 0.2% yeast powder, 0.5% glucose, 6% starch (liquefied by α-amylase), NaNO 3 0.2%, KH 2 PO 4 0.1%, ZnSO 4 ·7H 2 O 0.05% and CuSO 4 ·5H 2 O0.01%.

[0060] The titer obtained by using the above medium three for fermentation was 13 μg / ml.

Embodiment 2

[0062] Taking the basal medium formula as a control, under the condition that the carbon source, inorganic salt and other components remain unchanged, keep other nitrogen sources unchanged, explore the amount of corn steep liquor, adjust the corn steep liquor dosage from 1% to 1.2%, and shake the flask After 8 days of fermentation, the fermentation unit was measured by high performance liquid phase.

[0063] Table 2

[0064] Amount of corn syrup (%) 1 1.2 Relative potency 1.00 1.12

[0065] The optimized formula is: 1.2% corn steep liquor, 3.5% cold-pressed soybean powder, 0.2% yeast powder, 0.5% glucose, 6% starch (liquefied by α-amylase), NaNO 3 0.2%; KH 2 PO 4 0.1%, ZnSO 4 ·7H 2 O 0.05%; CuSO 4 ·5H 2 O0.01%.

Embodiment 3

[0067] Taking the basal medium formula as a control, under the condition of keeping the carbon source, inorganic salt and other components constant, and keeping other nitrogen sources constant, explore the amount of corn steep liquor, adjust the corn steep liquor dosage from 1.2% to 1.4%, and shake the flask After 8 days of fermentation, the fermentation unit was measured by high performance liquid phase.

[0068] table 3

[0069] Amount of corn syrup (%) 1.2 1.4 Relative potency 1 1.09

[0070] The optimized formula is: 1.4% corn steep liquor, 3.5% cold-pressed soybean powder, 0.2% yeast powder, 0.5% glucose, 6% starch (liquefied by α-amylase), NaNO 3 0.2%, KH 2 PO 4 0.1%, ZnSO 4 ·7H 2 O 0.05%; CuSO 4 ·5H 2 O0.01%.

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Abstract

The invention discloses a fermentation medium and a fermentation method for producing Bleomycin A5. The fermentation medium for producing the Bleomycin A5 comprises a carbon source, a nitrogen source,inorganic salt, and water; the content of the nitrogen source is 4.5-5.9%; the nitrogen source consists of soybean cake powder and corn steep liquor; and the content of the corn steep liquor is greater than 0.75% and less than 2.2%, wherein the percentage is mass percentage calculated on basis of the mass of the fermentation medium. Being adopted for fermenting the Bleomycin A5, the fermentationmedium disclosed by the invention can greatly increase yield of the Bleomycin A5 (to 2.47 times compared with a fermentation medium in the prior art), and remarkably reduce fermentation cost; and thus, a good foundation is laid for industrial production of the Bleomycin A5.

Description

technical field [0001] The invention belongs to the technical field of industrial microorganisms, in particular to a fermentation medium and a fermentation method for producing Pingyangmycin. Background technique [0002] Shanghai No.3 Pharmaceutical Factory (Yu Kui, Xu Yongshou, Li Miaoer. Effect of Organic Nitrogen Sources on the Changes of Bleomycin Fermentation Components[J]. Microbiology Bulletin, 1980,7(4):14-17.) in 1976 It was pointed out that the addition of corn steep liquor could significantly increase the fermentation titer of Pingyangmycin, which was found to be related to the presence of spermidine in corn steep liquor. The conditions affecting the fermentation of Pingyangmycin were explored by shaking flask experiments. Glucose 0.5%, maltose 7%, NaCl 0.3%, NaNO 3 0.2%, KH 2 PO 4 0.1%, ZnSO 4 ·7H 2 O0.05%, CuSO 4 0.01%, pH6.5, 150ml / 750ml Erlenmeyer flask conditions carried out shake flask fermentation, and the result pointed out that corn steep liquor...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12N1/20C12R1/465
CPCC12P21/005C12P21/02C12N1/20
Inventor 李继安林惠敏卢雪欢张建斌李亚军邓旭郭瑞玲孟宪纬
Owner SHANGHAI INST OF PHARMA IND
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