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A kind of method for obtaining red potato mutant and used mutagenesis medium

A potato and culture medium technology, applied in the field of agricultural biology, can solve the problems of easy recovery of mutant strains, application limitations, etc., and achieve the effect of increasing the quantity

Active Publication Date: 2016-05-04
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, EMS mutagenesis and irradiation techniques are mainly used for mutagenesis of potato mutants. Due to the strong toxicity of EMS and the easy recovery of the obtained mutant strains, the application is limited.

Method used

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  • A kind of method for obtaining red potato mutant and used mutagenesis medium
  • A kind of method for obtaining red potato mutant and used mutagenesis medium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Weigh:

[0099] (1) Inorganic substances: potassium nitrate 2108mg, ammonium nitrate 1650mg, potassium dihydrogen phosphate 170mg, magnesium sulfate 370mg, calcium chloride 440mg, potassium iodide 0.83mg, boric acid 6.0mg, manganese sulfate 16.9mg, zinc sulfate 9.6mg, sodium molybdate 0.25mg, copper sulfate 0.025mg, cobalt chloride 0.025mg, ferrous sulfate 27.8mg, disodium edetate 37.3mg;

[0100] (2) Organic matter: hydrolyzed casein 100mg, yeast extract 250mg, hydrolyzed milk protein 100mg, inositol 100mg, niacin 0.5mg, pyridoxine hydrochloride 0.5mg, thiamine hydrochloride 0.1mg, glycine 2mg;

[0101] (3) Plant hormones: 6-benzylaminoadenine 1.0mg, naphthaleneacetic acid 0.005mg, kinetin 0.5mg, indoleacetic acid 1.0mg, zeatin 4mg;

[0102] (4) 30g of sucrose;

[0103] (5) Beilining 4g;

[0104] Preparation method: fully dissolve the weighed components with deionized water, adjust the volume to 1L, adjust the pH to 5.8-6.0, subpackage, and sterilize at 121°C for 15...

Embodiment 2

[0107] Weigh:

[0108] (1) Inorganic substances: potassium nitrate 1900mg, ammonium nitrate 1650mg, potassium dihydrogen phosphate 170mg, magnesium sulfate 370mg, calcium chloride 440mg, potassium iodide 0.83mg, boric acid 6.0mg, manganese sulfate 16.9mg, zinc sulfate 9.6mg, sodium molybdate 0.25mg, copper sulfate 0.025mg, cobalt chloride 0.025mg, ferrous sulfate 27.8mg, disodium edetate 37.3mg;

[0109] (2) Organic matter: inositol 100mg, niacin 0.5mg, pyridoxine hydrochloride 0.5mg, thiamine hydrochloride 0.1mg, glycine 2mg;

[0110] (3) Phytohormones: 6-benzylaminoadenine 1.0mg, naphthaleneacetic acid 0.01mg, kinetin 0.5mg, indoleacetic acid 1.0mg, zeatin 4mg;

[0111] (4) 30g of sucrose;

[0112] (5) Beilining 4g;

[0113] Configuration method: same as embodiment 1.

Embodiment 3

[0115] Weigh:

[0116] (1) Inorganic substances: potassium nitrate 1900mg, ammonium nitrate 1600mg, potassium dihydrogen phosphate 175mg, magnesium sulfate 360mg, calcium chloride 430mg, potassium iodide 0.83mg, boric acid 6.0mg, manganese sulfate 16.9mg, zinc sulfate 9.6mg, sodium molybdate 0.25mg, copper sulfate 0.025mg, cobalt chloride 0.025mg, ferrous sulfate 27.8mg, disodium edetate 37.3mg;

[0117] (2) Organic matter: inositol 100mg, niacin 0.5mg, pyridoxine hydrochloride 0.5mg, thiamine hydrochloride 0.1mg, glycine 1mg;

[0118] (3) Plant hormones: 6-benzylaminoadenine 1.0mg, naphthaleneacetic acid 0.005mg, kinetin 0.5mg, indoleacetic acid 1.0mg, zeatin 4mg;

[0119] (4) 30g of sucrose;

[0120] (5) Beilining 3.5g;

[0121] Configuration method: same as embodiment 1.

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Abstract

The invention relates to a method for acquiring a red potato mutant and a mutagenesis medium used by the method and belongs to the technical field of agriculture Biotechnology. The mutagenesis medium is obtained by use of 30-40mg / L of pingyangmycin in a regenerated medium. The method comprises the following steps of putting purple potato square pieces into the mutagenesis medium, carrying out culture for 15 days, transferring the purple potato square pieces to a regenerated medium, and carrying out culture under culture conditions of a temperature of 22+ / -2 DEG C, illumination intensity of 700lx, illumination time of 8h / d and relative humidity of 65-75%. Through the mutagenesis medium and method, after mutagenesis for 15 days, the purple potato can produce mutation and through further culture, a potato regeneration seedling which can produce red stem tubers is obtained.

Description

technical field [0001] The invention relates to a method for obtaining red potato mutants and the mutagenesis medium used, belonging to the field of agricultural biotechnology. Background technique [0002] Potato is the fourth largest food crop in the world and an important part of my country's food security system. Potatoes are rich in nutrients and are known as "underground apples". Potatoes are rich in antioxidants, the main ones being phenols, ascorbic acid, carotenoids, tocopherols, alpha-lipoic acid, and selenium. Antioxidants of polyphenols are mainly L-tyrosine, caffeic acid, chlorogenic acid and so on. In addition to the above-mentioned antioxidants, colorful potatoes are also rich in health-care natural anthocyanins with obvious antioxidant effects. Its content is two to three times that of ordinary white-flesh potatoes, or even higher. In addition, colored potatoes not only have the excellent characteristics of ordinary potatoes, but also have bright meat and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 刘芳杨元军王培伦董道峰陈广侠马蕾
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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