Method for cultivating new Rosa sp. variation variety by using chemical mutagenic agent

A chemical mutagen and new variety technology, applied in the field of agriculture, can solve the problems of high plant death rate and low rose mutation, and achieve the effects of high plant mutation rate, good mutation effect and good mutation effect

Inactive Publication Date: 2021-11-02
TAISHAN RES INST OF FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country has not carried out systematic research on rose tree resources so far, and is still in the stage of conventional breeding at a low level of utilization. Artificially inducing mutants is one of the important ways to obtain new plant germplasm, but at present artificially inducing mutants to obtain rose tree resources There are few studies, and the commonly used induction method has low variation in roses, and the plant mortality rate is high during the variation process

Method used

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  • Method for cultivating new Rosa sp. variation variety by using chemical mutagenic agent
  • Method for cultivating new Rosa sp. variation variety by using chemical mutagenic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) The rose seeds collected from the end of October to the beginning of November are sown in a seedling tray equipped with a seedling substrate after being germinated in sand, and routinely managed after emergence; the seedling substrate is composed of peat soil, sawdust, and functional fillers in mass ratio It is mixed in the ratio of 4:3:2;

[0025] (2) Pingyangmycin and pH are mixed with phosphate buffer solution of 7 to prepare Pingyangmycin solution, and Pingyangmycin and MS liquid medium are mixed to prepare MS nutrient solution; Pingyangmycin solution, Pingyangmycin in MS nutrient solution The concentration of the rose seedlings was 60 μg / ml; the seedlings were unearthed in early April, and when the cotyledons were flattened and the true leaves were exposed, the rose seedlings were put into the MS nutrient solution prepared above for hydroponics treatment, and at the same time, the rose seedlings dipped in the above-mentioned Pingyangmycin The absorbent cotton b...

Embodiment 2

[0028] (1) The rose seeds collected from the end of October to the beginning of November are sown in a seedling tray equipped with a seedling substrate after being germinated in sand, and routinely managed after emergence; the seedling substrate is composed of peat soil, sawdust, and functional fillers in mass ratio It is mixed in the ratio of 6:5:2;

[0029] (2) Pingyangmycin and pH are mixed with phosphate buffer solution of 7 to prepare Pingyangmycin solution, and Pingyangmycin and MS liquid medium are mixed to prepare MS nutrient solution; Pingyangmycin solution, Pingyangmycin in MS nutrient solution The concentration of the rose seedlings was 60 μg / ml; the seedlings were unearthed in early April, and when the cotyledons were flattened and the true leaves were exposed, the rose seedlings were put into the MS nutrient solution prepared above for hydroponics treatment, and at the same time, the rose seedlings dipped in the above-mentioned Pingyangmycin The absorbent cotton b...

Embodiment 3

[0032] (1) The rose seeds collected from the end of October to the beginning of November are sown in a seedling tray equipped with a seedling substrate after being germinated in sand, and routinely managed after emergence; the seedling substrate is composed of peat soil, sawdust, and functional fillers in mass ratio It is mixed in the ratio of 6:4:3;

[0033] (2) Pingyangmycin and pH are mixed with phosphate buffer solution of 7 to prepare Pingyangmycin solution, and Pingyangmycin and MS liquid medium are mixed to prepare MS nutrient solution; Pingyangmycin solution, Pingyangmycin in MS nutrient solution The concentration of the rose seedlings was 60 μg / ml; the seedlings were unearthed in early April, and when the cotyledons were flattened and the true leaves were exposed, the rose seedlings were put into the MS nutrient solution prepared above for hydroponics treatment, and at the same time, the rose seedlings dipped in the above-mentioned Pingyangmycin The absorbent cotton b...

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Abstract

The invention discloses a method for cultivating a new Rosa sp. variation variety by a using chemical mutagenic agent. The method specifically comprises the following steps of: storing Rosa sp. seeds in sand, germinating, sowing in a hole tray, mixing pingyangmycin and a phosphate buffer solution to prepare a pingyangmycin solution, and mixing the pingyangmycin and an MS liquid culture medium to prepare an MS nutrient solution; when young seedlings come out of the soil, cotyledons are flattened and true leaves germinate, putting the young seedlings into the MS nutrient solution for carrying out hydroponic treatment, treating the growing points of the stem tips of the young seedlings by adopting the pingyangmycin solution, treating once in the morning, at noon and at night every day, continuously treating for 2-4 days, washing the growing points of the stem tips of the young seedlings and the roots of the young seedlings with clear water after the treatment is completed, and transferring the young seedlings into the MS liquid culture medium for culture; and after the Rosa sp. is treated for one month, the leaves of the Rosa sp. are gradually varied, and after variation is stable, plants with variation characters are subjected to conventional management in a greenhouse. According to the method, the chemical mutagenic agent is adopted to treat the growing points of the stem tips of plants and the root tips of the plants, operation is easy, a plant mutagenesis rate is high, a mutagenesis effect is good, and the survival rate of the plants is high.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a method for cultivating new varietal varieties of roses by using a chemical mutagen. Background technique [0002] Rosa is a general term for some plants of the genus Rosa, mainly referring to varieties and horticultural varieties of Rosa vine. Most of them are vine-like small flowers climbing fences, with large thorns and generally hooks; alternate leaves, odd-numbered pinnate compound leaves, 5-9 leaflets, toothed leaf margins, flat but pubescent leaves; flowers are often There are 6-7 flowers in the family, which are panicle-shaped corymbs, born on the top of the branches, with a flower diameter of about 3 cm, and only bloom once a year. It is an important garden ornamental plant. Rose is cold-resistant and can be used as medicine, and the rose porridge made from it has good nutritional value. my country has not carried out systematic research on rose tree resources s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H4/00
CPCA01H1/06A01H4/002A01H4/008
Inventor 李承秀于永畅程甜甜孙忠奎张林燕语张安琪任红剑杜辉仲凤维鹿苹朱翠翠
Owner TAISHAN RES INST OF FORESTRY
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