Method for preparing high-oleic oilseed rape material by utilizing artificially synthesized brassica napus

A Brassica napus, artificial synthesis technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of large selection and breeding workload, narrow selection of germplasm and other problems

Inactive Publication Date: 2017-05-31
SOUTHWEST UNIVERSITY
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing high oleic rapeseed materials by artificially synthesizing Brassica napus, aiming to solve the problem that the creation of existing high oleic rapeseed varieties mainly focuses on mutation breeding, genetic engineering and conventional breeding methods. The range of quality selection is relatively narrow, and the workload of selection is large

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  • Method for preparing high-oleic oilseed rape material by utilizing artificially synthesized brassica napus

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Embodiment 1

[0025] The method for preparing high oleic rapeseed materials by artificially synthesizing Brassica napus provided by the embodiment of the present invention utilizes artificially grown Brassica napus (10D130) and high-quality, low-yield, high-yielding Brassica napus (Zhongshuang No. 11) to artificially create high-oleic acid materials; specifically include the following steps:

[0026] 1. In the spring of 2010, 10D130 (artificially synthesized Brassica napus with an erucic acid content of more than 45% and an oil content of more than 40%) was used at the Xiema Experimental Base of Chongqing Rapeseed Engineering Center, Southwest University, Beibei District, Chongqing City (where all field experiments were conducted). ) and double-low high-quality Brassica napus variety Zhongshuang 11 (natural Brassica napus with erucic acid content of less than 1%, oil content of more than 45%, and oleic acid content of about 60%) to obtain the first-generation hybrid F1.

[0027] 2. Spring 2...

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Abstract

The invention discloses a method for preparing a high-oleic oilseed rape material by utilizing artificially synthesized brassica napus. The method comprises the steps of utilizing the artificially synthesized brassica napus for hybridizing with natural brassica napus, obtaining a great deal of variations through mass exchange and recombination among filial generation chromosomes, and deficiency, insertion, transposition and inactivation of gene sequences, preventing brassica napus oleic acid synthesis from transforming to linoleic acid and linolenic acid, and utilizing mutation accumulation to acquire a high oleic acid material. According to the method provided by the invention, in earlier generation, oleic acid characteristics have no need to be selected, so that the breeding period can be shortened through a generation-adding technique; in earlier generation, a planting group contains about 300 plants (strains), so that compared with thousands of single plants (strains) for mutation breeding, the work load is smaller; during orientation selection, the selective pressure is increased by successive generations, so that the frequency of acquiring different types of high oleic acid is increased, and the breeding efficiency is improved. According to the method provided by the invention, the breeding selection group is small, the work load is small, the variation range is large, and high oleic acid brassica napus genetic resources with the oleic acid content being higher than 80 percent are obtained.

Description

technical field [0001] The invention relates to the technical field of new material breeding of Brassica napus, in particular to a method for preparing high oleic rapeseed material by artificially synthesizing Brassica napus. Background technique [0002] At present, high oleic rapeseed oil is a healthy and nutritious oil, which can reduce the content of low-density lipoprotein in human arteries and blood vessels, and has no effect on the content of high-density lipoprotein, thereby reducing the formation of cholesterol and effectively preventing human cardiovascular diseases ; High oleic rapeseed oil is more resistant to storage and has a longer shelf life; in addition, high oleic rapeseed oil can effectively carry out methylation, which is beneficial to the production of biodiesel. Therefore, high oleic acid rapeseed oil plays a very important role in health care, food processing and industrial application, and high oleic acid rapeseed breeding has become an important rese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 周清元李加纳崔翠唐章林徐新福林呐樊晋华王倩
Owner SOUTHWEST UNIVERSITY
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