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SNP molecular markers related to lead accumulation in maize plants and their applications

A molecular marker, corn technology, applied in the field of molecular genetic markers, can solve the problems of complex lead response stress mechanism, little key gene mining work, and slow research progress, so as to speed up the breeding of low lead accumulation, reduce the workload of breeding, The effect of shortening the breeding time

Active Publication Date: 2022-07-01
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stress response mechanism of lead in maize is complex and the current research progress is slow, and there are still few key genes that have high utilization value in breeding practice

Method used

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  • SNP molecular markers related to lead accumulation in maize plants and their applications
  • SNP molecular markers related to lead accumulation in maize plants and their applications
  • SNP molecular markers related to lead accumulation in maize plants and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Obtaining the molecular marker of the gene Zm00001d034674 related to the lead accumulation trait.

[0027] The method to obtain is as follows:

[0028] (1) Identification of associated population phenotype: The present invention collected 305 temperate, tropical and subtropical maize inbred lines from China, the United States, Mexico and other places. Coupled plasma mass spectrometry, ICP-MS) was used to determine the lead accumulation in plants, and three biological replicates were set up. Joint analysis of variance showed that the genotypes of different materials were significantly different.

[0029] Table 1: Phenotypic Joint ANOVA

[0030]

[0031] (2) Obtaining the genotype of the associated population: using transcriptome sequencing to obtain the genotype of the population.

[0032] (3) Genotype and phenotype association analysis: Using the MLM model of Tassel software, the genome-wide association analysis was carried out considering the populatio...

Embodiment 2

[0035] Example 2: Association analysis between gene expression and phenotype

[0036] (1) Acquisition of gene expression: The open source software Kallisto was used to quantify the expression of genes and transcripts in the population material.

[0037] (2) Association analysis between gene expression and phenotype: Using R language and linear regression model, the degree of association between gene expression and phenotype was identified. At p<0.05 level, the expression of Zm00001d034674 gene was significantly correlated with lead accumulation in maize plants under stress, with a significant P value of 0.0032.

[0038] The results further confirmed that the expression of this gene was related to the accumulation of lead in the aerial part of maize under heavy metal lead stress.

Embodiment 3

[0039] Example 3: ANOVA based on marker type

[0040] (1) Phenotype grouping: Based on the genotypes of the marker in different inbred lines, the test materials were divided into two groups, in which the inbred lines with marker genotype C were recorded as group A, and those with marker genotype T were recorded as group A. Do group B.

[0041] (2) Analysis of variance: Analysis of variance was performed using Excel software. The results showed that there were significant differences between the two groups. The mean value of group A was 66.65, and the mean value of group B was 89.52. table 3.

[0042] Table 3: ANOVA

[0043]

[0044] like figure 1 , the distribution of lead ion content in plants of different genotypes shows that the lead ion content of the C genotype material is significantly different from that of the T genotype material, and its mean and maximum values ​​are both smaller than those of the T genotype material.

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Abstract

The invention discloses a SNP molecular marker related to lead accumulation in maize plants and its application. The invention proposes a SNP molecule related to the lead accumulation in maize plants, the SNP molecule is located on the maize chromosome 1, and can be used for screening or cultivating varieties with low lead accumulation, shortening the breeding time of varieties with low lead accumulation in maize, and reducing It also provides a theoretical basis for the corresponding mechanism of plant lead stress, which is of great significance for accelerating the breeding of low lead accumulation in maize under heavy metal stress.

Description

technical field [0001] The invention relates to an SNP molecular marker related to lead accumulation in maize plants and an application thereof, and belongs to the technical field of molecular genetic markers. Background technique [0002] China is the second largest maize producer in the world, and abiotic stress has a great impact on the yield and quality of maize. The heavy metal lead is harmful to plant growth, and as abiotic stress, it is easy to cause adverse effects on maize breeding. At present, soil is increasingly polluted by heavy metals, and lead pollution has the characteristics of great harm and high concealment. Due to the accumulation of lead in plants, it is very important to create and cultivate high-quality maize varieties with low lead accumulation. [0003] Research and production practice have shown that maize lead accumulation-related traits are a complex quantitative trait, and plant tissue lead accumulation as an indicator of plant lead accumulatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 沈亚欧刘鹏张晓祥马浪浪
Owner SICHUAN AGRI UNIV
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