Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Purifying method for copper chelate of pingyangmycin and same-family compounds thereof

A technology of pingyangmycin and copper chelate, which is applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of inability to achieve rapid separation, low purity of the final product, and low separation degree, etc. Achieve the effect of improving the quality of the final product, good rigidity and safety

Active Publication Date: 2014-12-24
哈尔滨莱博通药业有限公司
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The packing has a high swelling rate, and its volume changes greatly in different salt solutions. It is a non-rigid packing and cannot be pressurized. In order to achieve a high degree of separation, the column diameter ratio is generally above 30, which makes the cycle of each cycle very long. , unable to achieve fast separation, the overall efficiency is low
At the same time, because the separation degree of CM-sephadex-C-25 itself is not very high, some impurities cannot be removed, so that the purity of the final product is not high and the quality is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Purifying method for copper chelate of pingyangmycin and same-family compounds thereof
  • Purifying method for copper chelate of pingyangmycin and same-family compounds thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 3.0 grams of Pingyangmycin copper chelate crude product (content 55%), dissolve it with ultrapure water, and go up to the UniPS equilibrated with ultrapure water in advance TM -30 Chromatographic column with a series of fillers, the diameter of the filler is 2.5cm, and the height is 40cm. Chromatography flow rate 0.8ml / (min cm 2 ). Use ethanol-water system to elute with 0%-15% linear gradient for 4 column volumes (here refers to the volume of the filler, the same below), then wash 1 column volume with 15% ethanol, and finally wash the column with 30% ethanol All the ribbons on the top are all down. The single chromatography time is about 4.5 hours, and the fractions with a purity of more than 85% are collected, concentrated to dryness under reduced pressure, and the purity and recovery are measured. The purity is 87.6%, and the recovery rate is 82%. The samples were reuploaded to a UniPS equilibrated with ultrapure water TM -30 The chromatographic column w...

Embodiment 2

[0036] Take 10 grams of Pingyangmycin copper chelate crude product (content 48%), dissolve with ultrapure water, and go up to the UniPS that is equilibrated with ultrapure water in advance TM -40 Chromatographic column with a series of fillers, the diameter of the filler is 2.5cm, and the height is 40cm. Chromatography flow rate 0.8ml / (min cm 2 ). Use methanol-water system to elute 4 column volumes according to a linear gradient of 0%-15%, then wash 1 column volume with 15% methanol, and finally use 50% methanol to remove all the color bands on the column. The single chromatography time is about 4.5 hours, and the fractions with a purity of more than 85% are collected, concentrated to dryness under reduced pressure, and the purity and recovery are measured. The purity is 86.1%, and the recovery rate is 76%. The samples were reuploaded to a UniPS equilibrated with ultrapure water TM -40 The chromatographic column with a series of fillers was operated the same as above t...

Embodiment 3

[0038] Get 40 grams of pingyangmycin copper chelate crude product (content 56%), dissolve with ultrapure water, and go up to equilibrate UniPS with 10% methanol in advance TM -40 Chromatographic column with a series of fillers, the diameter of the filler is 5.0cm, and the height is 150cm. Chromatography flow rate 1.8ml / (min cm 2 ). Elute isocratically with 10% aqueous methanol for a total of 4 column volumes, and finally wash the column with 50% methanol. The single chromatography time was 6 hours, and the fractions with a purity of more than 85% were collected, concentrated to dryness under reduced pressure, and the purity and recovery were measured. The purity is 89.3%, and the recovery rate is 84%. Reload the sample to a UniPS equilibrated with 10% methanol TM -40 The chromatographic column with a series of fillers is the same as the above operation, and the fractions with a purity of more than 97% are collected, combined, concentrated under reduced pressure and eva...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a purifying method for copper chelate of pingyangmycin and of same-family compounds thereof. The purifying method comprises the following steps: independently using monodisperse polystyrene / divinyl benzene chromatography media or combining the monodisperse polystyrene / divinyl benzene chromatography media with monodisperse polymethacrylate hybrid type cation exchange chromatography media so as to use as stuffing to carry out chromatography. The purifying method can be used for overcoming the defects that the original process is long in period, low in efficiency and low in product purity to obtain the copper chelate of high-purity pingyangmycin and same-family compounds thereof, wherein the purity is measured by virtue of an HPLC (high performance liquid chromatography), and the purity is over 97% in area percentage. After removing copper from the copper chelate, the high-purity pingyangmycin and same-family compounds thereof are obtained, so that the safety of clinical medication is improved.

Description

technical field [0001] The invention belongs to the technical field of medicinal chemistry and separation and purification, and in particular relates to a method for purifying copper chelates of pingyangmycin and its homologous compounds. Background technique [0002] Pingyangmycin and its congeners Boanmycin and Boningmycin are anti-tumor antibiotics independently developed by Chinese scholars, among which Pingyangmycin and Boanmycin have been put into clinical application. They all belong to the bleomycin family. The main components of imported bleomycin are bleomycin A2 and B2, Pingyangmycin has the same structure as bleomycin A5, bleomycin has the same structure as bleomycin A6, and bleomycin Boanmycin is the acetylated product of the free amino group of the terminal ammonia body of boanmycin. Pingyangmycin is now recorded in the second part of the Pharmacopoeia of the People's Republic of China (2010 edition), and it is a commonly used anticancer drug in clinical prac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K9/00C07K1/18C07K1/16
Inventor 曾佳烽潘俊芳王启运吴光明于国庆冯子群张士花黄靖安
Owner 哈尔滨莱博通药业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com