Method for improving salt-tolerant properties of Brassicaceae crops

A technology of cruciferous crops and salt tolerance, which is applied in botany equipment and methods, horticultural methods, plant genetic improvement, etc., and can solve the problem of less research on salt tolerance

Active Publication Date: 2010-06-16
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, most of the research on the breeding of such crop cultivars involves heat resistance, cold resistance and improving the taste quality, and there are few studies on its salt tolerance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1: Salt tolerance treatment and germination screening of cabbage seeds

[0076] Carefully select green vegetable seeds with full grains and complete seed coats, and add a drop of 0.1% HgCl of Tuwen-20 2 After 10 minutes of sterilization, rinse with sterile water and dry the water, soak the seeds in a dark incubator at 25°C for 24 hours with 120mg / L Pingyangmycin at a dosage of 1ml / grain, take it out and rinse with sterile water Clean, inoculate on 1 / 2MS medium containing 0.8% NaCl, add 30g / L sucrose, 6.5g / L agar, pH 5.6, culture at 25±1°C, 10-12 hours of light per day, 5 After ~7 days, select the seeds that can germinate normally and have true leaves drawn out as the primary strains for salt-tolerant germination.

Embodiment 2

[0077] Example 2: Initial in vitro screening of salt-tolerant strains

[0078] Cut off the above-mentioned buds from which the true leaves have been extracted, inoculate them in 1 / 2MS medium with 30g / L sucrose, 6.5g / L agar, and pH 5.6, and cultivate them at 25±1°C with 10-12 hours of light per day After 20 to 25 days, when it grows to 4 to 5 true leaves, remove the glass seedlings and abnormal seedlings with abnormal true leaves, cut off the normal growing buds together with 4 true leaves, transfer them to 0.8% NaCl, add sucrose 30g / L, agar 6.5g / L, 1 / 2MS medium with a pH of 5.6, under the condition of 25±1°C, 10-12 hours of light per day, after stress culture for 25-30 days, select the surviving buds as the first in vitro Screen salt-tolerant strains, transfer to 1 / 2MS medium with 30g / L sucrose, 6.5g / L agar, PH 5.6, and carry out 25-30 days at 25±1℃, under the condition of 10-12 hours of light per day recovery culture.

Embodiment 3

[0079] Example 3: Secondary in vitro screening of salt-tolerant strains

[0080] Cut off the terminal buds with 3 to 4 leaves of the salt-tolerant strains that have been screened in vitro for the first time after recovery and culture, and transfer them to 1 / 2 of sucrose containing 0.8% NaCl, 30 g / L of sucrose, 6.5 g / L of agar, and PH of 5.6. 2MS medium, under the condition of 25±1°C, 10-12 hours of light per day, after stress culture for 25-30 days, conduct a second in vitro screening: observe the growth status of these buds on the NaCl-containing medium, and eliminate the leaves that turn yellow , wilting or vitrified stems, water-soaked buds, leaving sprouts with dark green leaves and strong stems, added 6-BA 0.2mg / L, KT 0.2mg / L, sucrose 30g / L, Agar 6.5g / L, 1 / 2MS medium with a pH of 5.6, at 25±1°C, under the condition of 10-12 hours of light per day, carry out recovery and proliferation culture according to the strain, and subculture once every 25-30 days. A total of 3 time...

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PUM

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Abstract

The invention provides a method for improving salt-tolerant properties of Brassicaceae crops, which comprises the following steps of: processing sterile seeds of the crops by using pingyangmycin and the like; performing NaCl stress screening for layer-upon-layer elimination during the germination period of the seeds, the isolated culture period of the stem apex and the induction period of rhizogenesis respectively; and after strains are obtained, performing extended propagation according to the strains, and adding the NaCl stress screening for one time after subculture every three times in the process of extended propagation to increase the obtaining frequency of the salt-tolerant strains of the crops and accelerate the process of the salt-tolerant breeding of the crops. The method is simple to implement and easy to master, the whole screening process is performed under the controlled condition of completely-consistent laboratory rooms, so that screening results are real and reliable, and the strains obtained by screening have high stability.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and relates to a method for improving the salt tolerance of cruciferous crops with seeds as the propagation mode. Background technique [0002] my country's coastline is long, and various coastal saline soils are widely distributed, with a total area of ​​about 5×106hm2. With the rapid development of the national economy and society, the contradiction between population growth and arable land reduction has become increasingly prominent. As an important land reserve resource, coastal saline soil needs to be developed and utilized urgently. Therefore, it is necessary to cultivate new salt-tolerant crops and improve crop salt-tolerant Sexuality studies are gaining in importance. [0003] Cruciferous crops that reproduce by seeds, such as green vegetables, which are widely grown in the coastal provinces of southern my country and eaten by the public, have low salt tolerance and lack salt-toler...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00A01H1/04A01H4/00
Inventor 王亦菲黄剑华陆瑞菊陈志伟何婷孙月芳
Owner SHANGHAI ACAD OF AGRI SCI
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