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Method for carrying out bacteriostatic culture after genetic transformation of arabidopsis thaliana

A technology of genetic transformation and cultivation method, which is applied in the field of plant tissue culture, can solve the problems of cumbersome working procedures and heavy workload, and achieve the effect of reducing energy consumption, reducing workload, and strong practicability

Inactive Publication Date: 2014-07-30
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

The above work procedures are cumbersome and heavy workload

Method used

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  • Method for carrying out bacteriostatic culture after genetic transformation of arabidopsis thaliana
  • Method for carrying out bacteriostatic culture after genetic transformation of arabidopsis thaliana

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Embodiment 1

[0026] Embodiment one: the bacteriostatic culture method after Arabidopsis genetic transformation

[0027] The bacteriostatic culture method after Arabidopsis genetic transformation comprises the following steps:

[0028] 1. Screening and preparation of bacteriostatic agents: select 4 kinds of medicaments with 3 concentrations each, use the L9(3)4 orthogonal table to carry out the permutation test, and calculate the contamination rate after 15 days of culture medium preparation. The results are shown in Table 3.

[0029] Table 3 Effects of different agents and concentrations on medium contamination

[0030]

[0031]

[0032] According to the K value and R value in Table 3, the primary and secondary factors found out are respectively: sodium hypochlorite, dimethyl fumarate, calcium propionate, mature garlic juice; the best combination is: 50ppm sodium hypochlorite+6ppm Dimethyl fumarate + 50ppm calcium propionate + 0.05-0.2ml mature garlic juice.

[0033] The serial num...

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Abstract

The invention relates to a method for carrying out bacteriostatic culture after genetic transformation of arabidopsis thaliana. The method comprises preparation of a bacteriostatic agent, culture vessel disinfection, agrobacterium tumefaciens transformation, preparation of a screening culture medium, screening culture and detection of seedlings with resistance. The method for carrying out bacteriostatic culture after genetic transformation of arabidopsis thaliana has the advantages that the culture vessel and the culture medium are unnecessary to undergo high temperature and high pressure sterilization, thus reducing the workload and the energy consumption, simplifying the links of culture after genetic transformation of arabidopsis thaliana and reducing the cost of culture after genetic transformation of arabidopsis thaliana; the method for carrying out bacteriostatic culture after genetic transformation of arabidopsis thaliana is simple to operate, is only characterized by adding the bacteriostatic agent after preparation according to different culture medium formulas, and has strong practicability and good popularization property; compared with the conventional methods, the method for carrying out bacteriostatic culture after genetic transformation of arabidopsis thaliana has the advantage that the cost can be reduced by more than 10%.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a bacteriostatic culture method after genetic transformation of Arabidopsis thaliana, which belongs to the field of biological technology. Background technique [0002] Arabidopsis thaliana belongs to the Brassicaceae Arabidopsis genus, which has all the characteristics of flowering plants, and has the advantages of small genome, simple structure, small body, short growth cycle, high reproduction coefficient, and white flower pollination. It is a model species for "Drosophila in the plant kingdom" and molecular biology research. The Arabidopsis genome is small, and many genes are single-copy, so genes are relatively easy to clone and analyze. Therefore, more and more attention has been paid to plant genetic engineering and crop genetic improvement. The model species of Arabidopsis has been selected for the verification of gene function in many crops. [0003] In the gene functi...

Claims

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Application Information

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IPC IPC(8): C12N15/84A01H5/00
Inventor 林庆良许莉萍陈晓英高世宇
Owner FUJIAN AGRI & FORESTRY UNIV
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