Transgenic cultivation method of rice dedicated for starch medium

A cultivation method and starch-based technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of low starch coagulation resistance, unstable colloidal structure, difficult direct or modified utilization, etc., to achieve good coagulation The effect of glue properties

Inactive Publication Date: 2012-07-11
ZHEJIANG UNIV
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Problems solved by technology

[0004] Starch is a nutrient stored in plants and an important part of food. Rice contains 62% to 86% starch, but the starch solidification resistance of ordinary rice varieties is very low, and the colloidal structure is unstable, so it is difficult to directly or modify it.

Method used

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  • Transgenic cultivation method of rice dedicated for starch medium

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Embodiment 1

[0032] The mature embryos of the rice variety "Jiayu 948" with an amylose content of 13.6% were used as explants, inoculated into the induction medium, and induced to form callus under the conditions of 25±1°C and light intensity of 3000 lux;

[0033] The callus that was induced to form for 2 weeks was transferred to the subculture medium, and subcultured until the callus entered the doubling phase;

[0034] The doubly proliferated callus was pre-cultured for 2 days at 25±1°C, under a dark condition protected from light;

[0035] Soak the pre-cultured calli (approximately 500 petri dishes, containing more than 2000 pieces of callus) in the Agrobacterium suspension for 10 minutes, then discard the bacterial solution, blot dry with sterile filter paper, and inoculate in On the co-culture medium, co-cultivate for 2 days at 25±1°C in dark conditions protected from light;

[0036] Take the above-mentioned co-cultured callus, wash and dry it, then transfer it to the screening mediu...

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Abstract

The invention relates to a transgenic cultivation method of rice dedicated for a starch medium. The method comprises the following steps: a mature embro of a rice species with a low content of alpha amylose is taken as an explant and is induced to form calluses; the calluses are subcultured to enter a proliferation period and are preculutured under a dark condition; the calluses are co-cultivated with agrobacterium tumefaciens for transformation and then transferred into a screening culture medium for being screened for three tims; resistant calluses are taken to be induced and differentiated into seedlings, and histochemical stain and molecular identification are performed to resistant plants to identify the differentiated plants containing target genes; the starch gelanitization property of selected transgenic plants is identified, and the transgenic plants which have the starch gelatinized and can form semi-solid gel after being cooled are selected and reserved; and expression stability of the starch gelatinization property of the plants is further evaluated, seed propagation is performed to the excellent plants with a stable starch gelatinization property, and finally dedicated rice for the starch medium is cultivated. The dedicated rice for the starch medium can be taken as a novel biological medium and be widely applied to scientific researches, food, medicine and chemical engineering.

Description

technical field [0001] The invention relates to a transgenic breeding method for special starch substrate rice. Background technique [0002] There are more than 1,000 tissue culture rooms nationwide, and tissue culture technology is widely used in nearly 400 kinds of economic plants such as crops, fruit trees, vegetables, forest trees, ornamental plants and Chinese medicinal materials, and has achieved huge social and economic benefits in terms of rapid propagation and detoxification. Due to the high cost, the commercial use of tissue culture is greatly restricted, and it cannot meet the market's demand for high-quality tissue culture seedlings and low-cost costs. Therefore, cost reduction is the first condition for more successful commercialization of tissue culture technology. [0003] In terms of reducing the cost of tissue culture medium, the method of increasing the reproduction coefficient is often used, but the increase of the reproduction coefficient leads to a dec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 吴殿星舒小丽张宁
Owner ZHEJIANG UNIV
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