Preparation method and application of stable Pickering emulsion based on zein/pullulan composite solid particles
A technology of zein and pullulan, which is applied in the directions of emulsion delivery, medical preparations containing inactive ingredients, and medical preparations containing active ingredients, etc., can solve the complex purification method and difficult to achieve multiple PFM protection and digestion problems, to achieve the effect of simple preparation method, improved bioavailability and stability, and good gel properties
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[0044] A kind of preparation method of Pickering emulsion based on zein / pullulan composite solid particle stabilization of the present invention, such as figure 1 shown, including the following steps:
[0045] (1) Preparation of zein solid particles: 2.4 g of zein was dissolved in 80 mL of 75% (v / v) ethanol aqueous solution and stirred evenly with a magnetic stirrer to form a stock solution. Then pour the stock solution into 160mL ultrapure water, and after stirring continuously for 30min at 25°C and 800rpm, the homogenized solution (particle dispersion) was rotary evaporated at 40°C to spin off excess ethanol and water Finally, a zein solid particle dispersion is prepared, and the mass ratio of the zein solid particle to the total volume of the dispersion is 2% (w / v, g / mL).
[0046] (2) Preparation of zein / pullulan composite solid particles with different mass ratios: dissolve pullulan polysaccharides of different qualities in ultrapure water and stir for 30 minutes to prepa...
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