Bacillus velezensis and screening culture method and application thereof

A technology of Bacillus Velez and Bacillus, applied in the field of environmental microorganisms, can solve the problem of single and fixed types of degrading bacteria, achieve remarkable treatment effects, good application prospects, and strong stress resistance

Active Publication Date: 2020-12-18
BC P INC CHINA NAT PETROLEUM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The degrading bacteria provided by these patents are all compound strains, and the types of degrading bacteria involved are relatively single and fixed.

Method used

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  • Bacillus velezensis and screening culture method and application thereof
  • Bacillus velezensis and screening culture method and application thereof
  • Bacillus velezensis and screening culture method and application thereof

Examples

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example 1

[0037](1) Isolation, purification and preservation of Bacillus velezensis B3-4 (referred to as "strain B3-4")

[0038] The collected human feces samples were cryopreserved and brought back to the laboratory. Take 5 g of feces samples, use the gradient dilution method, spread on the beef extract peptone medium, and incubate at a constant temperature of 30°C. After the colonies grew out, the colonies with different shapes were selected from the plate and streaked on the beef extract peptone plate. After culturing for 16 hours, pick a ring for repeated streak culture, combined with microscopic observation, until purification. The purified strains were stored in the test tube slant medium of beef extract peptone medium.

[0039] As shown in Figure 1, the isolated and purified strain B3-4 obtained in this example was cultured on beef extract peptone medium, and after 48 hours of culture, the colony was round, white, with a diameter of 0.5-1 mm, irregular edges, flat and moist. i...

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Abstract

The invention provides Bacillus velezensis and a screening culture method and application thereof. The classification name of the Bacillus velezensis is Bacillus velezensis B3-4, the strain is preserved in the China Center for Type Culture Collection on May 29 , 2020, and the preservation number is CCTCC NO: M 2020160. The screening culture method comprises the following steps that a sample iscollected and preserved at low temperature; a beef extract peptone culture medium is coated with diluent of the sample through a gradient dilution method, and constant-temperature culture is conducted at 15-50 DEG C; after the culture medium grows out bacterial colonies, the bacterial colonies with different forms are selected for streak culture; and then, a circle of bacterial colonies are selected for repeated streak culture, and microscopic observation is combined till the purified strain is obtained. The Bacillus velezensis can effectively degrade and remove organic matter and COD in human excrement and domestic sewage.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a strain of Bacillus velesii and its screening and cultivation method and application. Background technique [0002] As early as the early 2000s, the country put forward the strategic goal of toilet renovation, and regarded toilet renovation as an important project to improve the environment and create a healthy living environment. [0003] As the product of the "toilet revolution", the environmentally friendly toilets have been continuously developed. At present, there are two types of environmentally friendly toilets that are widely used: water flushing and water-free toilets. Due to the lack of resources in most areas of China, in recent years, free Flush-free biodegradable toilets with functions such as flushing and excrement recycling have received a lot of attention. However, technically, the emphasis is on the design of the equipment structure, and le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C02F11/02C02F101/30C12R1/07
CPCC12N1/02C12N1/20C02F3/34C02F11/02C02F2101/30C12R2001/07C12N1/205
Inventor 刘汉军陈雷李辉张薇陈立荣蒋学彬张敏李盛林闫瑞景周鋆文炜涛孟召伟吴思斯毛红敏徐梓培孙玉
Owner BC P INC CHINA NAT PETROLEUM CORP
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