A kind of microbiological method for screening dimethoate residues in food

A technology for food and screening, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems affecting the accuracy and precision of microorganisms, and the sensitivity, sensitivity and specificity of dimethoate pesticides that have not yet been screened High and low problems, to achieve the detection method is simple and easy, the sensitivity of dimethoate is improved, and the specificity is good.

Inactive Publication Date: 2011-12-21
温州医科大学仁济学院
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Problems solved by technology

However, the sensitivity and specificity of the working strain to the substance to be tested is the most critical issue affecting the accuracy and precision of the microbiological method
So far, there is no relevant report on the screening of working strains of Bifidobacteria with high sensitivity and specificity to dimethoate pesticides and its application in the screening of dimethoate residues in food

Method used

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  • A kind of microbiological method for screening dimethoate residues in food
  • A kind of microbiological method for screening dimethoate residues in food
  • A kind of microbiological method for screening dimethoate residues in food

Examples

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Effect test

Embodiment 1

[0035] Example 1 Isolation and identification of Bifidobacterium adolescentis LJM-001

[0036] Using the feces of a healthy young man in Zhejiang Province as an isolated sample, the Bifidobacterium adolescent LJM-001 described in the present invention was obtained by spreading and culturing on a modified MRS agar medium under anaerobic conditions at 37°C for 48 hours, and identified It is Bifidobacterium adolescent, which was preserved on August 17, 2010 in the General Microbiology Center of China Committee for Culture Collection of Microorganisms (Address: No. 3, Datun Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, postal code 100101), The classification is named Bifidobacterium adolescentis, and the preservation number is CGMCC No.4090.

[0037] The formula of the improved MRS agar medium: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium acetate 5g, K 2 HPO 4 2g, MgSO 4 7H 2 O 0.5g, MnSO 4 4H 2 O 0.2g, fru...

Embodiment 2

[0054] Example 2 Screening of dimethoate residues in Chinese cabbage samples

[0055] 1. Preparation of screening medium:

[0056] Peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium acetate 5g, K 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.5g, MnSO 4 4H 2 O 0.2g, fructooligosaccharide 3g, diammonium citrate 2g, Tween-80 1ml, agar 15g, chromogen 0.1g, distilled water 1L, heat and dissolve the above ingredients to correct pH6.5, autoclave at 115℃ for 15- 20 minutes.

[0057] The chromogenic reagent is 5-bromo-4-chloro-3-indole-β-D-galactoside (X-Gal).

[0058] 2. Bacterial suspension preparation:

[0059] Bifidobacterium adolescent LJM-001 was inoculated on the slant medium of nutrient agar, cultured anaerobically at 35±2°C for 24 hours, washed the bacterial lawn with sterilized normal saline, made a suspension, and tested it at a wavelength of 580nm in a 721 spectrophotometer Control the light transmittance to 0.8 and store in a refrigerator at 2-8°C.

[0060] 3. ...

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Abstract

The invention provides a bifidobacterium LJM-001 strain. The collection number of the bifidobacterium LJM-001 strain is CGMCC No.4090. The LJM-001 strain is hypersensitive to dimethoate and is a working strain for detecting dimethoate residues in food. The invention also provides a microbiological method for screening dimethoate residues in food. According to the size of antibacterial rings on a screening culture medium, whether the residual quantity of the dimethoate in food samples exceeds the standard or not can be judged. The method for screening the dimethoate residues in food has the characteristics of simpleness and convenience for operation, low cost, high sensitivity and suitability for sample screening, and the screening requirement of a large number of samples in the pesticide residue detection department in China is met.

Description

technical field [0001] The invention relates to a strain of bifidobacterium adolescent and its application as a working strain in the screening of dimethoate residues in food. Background technique [0002] Dimethoate is a highly efficient and moderately toxic organophosphorus pesticide with a chemical name of O, O-dimethyl-S-(N-methylcarbamoylmethyl) phosphorodithioate (C 6 h 12 NO 3 P.S. 2 ), because of its advantages of quick effect and low price, it has been widely used in my country for insecticide, bactericide and acaricide of crops such as vegetables and fruits. [0003] However, the excessive use of dimethoate makes it highly residual in vegetables and fruits, which poses a huge threat to people's health, so it has been used in limited quantities in our country. In January 2005, the Ministry of Health of the People's Republic of China and the Standardization Administration of China issued GB2763-2005, which stipulated that the maximum residue limits (maximum resid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12N1/20C12R1/01
Inventor 刘佳明杜季梅姚蔚林刚
Owner 温州医科大学仁济学院
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