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Eupenicillium javanicum strain, and screening culture method and use thereof

A technology of Penicillium penicillium and Penicillium penicillium, which is applied in the field of strains producing high-activity cellulase and its screening and cultivation, can solve the problems of restriction of the overall activity of cellulase, insufficient activity of cellulase, low activity, etc., and achieve Easy to operate, simple method, high activity effect

Inactive Publication Date: 2009-06-24
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the strains that can be used to produce cellulase and have strong cellulase decomposition ability mainly include: Trichoderma (Trichoderma) strains, Aspergillus (Aspergillus) strains, Penicillium (Penicillium) strains and Acremonium strains, but the cellulase activity produced by the strains of these strains is not high enough, even if the activity of a certain enzyme in the cellulase produced by the above strains can achieve better results , but due to the low activity of other constituent enzymes, it will also restrict the overall activity of cellulase

Method used

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  • Eupenicillium javanicum strain, and screening culture method and use thereof
  • Eupenicillium javanicum strain, and screening culture method and use thereof
  • Eupenicillium javanicum strain, and screening culture method and use thereof

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Effect test

Embodiment 1

[0026] A high-activity cellulase-producing strain ZN-205 was screened and isolated from rotting trees and soil in the Yuelu Mountain forest in Changsha. The specific method is as follows:

[0027] (1) Preliminary selection: Put the collected strain samples into a Erlenmeyer flask containing 50 mL enrichment medium, shake and culture them at 28°C and 150 r / min for 4 days, then spread them on the CMC-Na medium after dilution, After upside-down culture at 30°C for 4 days, a single colony was picked and diluted again until pure colonies were obtained. Spot the isolated pure strain on CMC-Na medium and culture it for 4 days, then stain it with 1 mg / mL Congo red solution for 30 minutes, then wash it with distilled water, and finally decolorize it with 1 mol / L NaCl solution for 30 minutes. The ratio of the size to the colony diameter was used as the screening standard. Separation and screening by Congo red plate (see image 3 ), obtained 8 colonies with larger ratios of transparent...

Embodiment 2

[0070] A kind of Penicillium javanese bacterial strain of the present invention, it is to produce cellulase by following steps:

[0071] Insert the strains that have been screened and cultivated into the seed medium, and cultivate them for 72 hours at 30°C and 175r / min to obtain the seed solution; insert the seed solution into the fermentation medium at an inoculum size of 5% (in the fermentation 20~50% of the volume of the fermentation medium in the bottle), the initial pH value of the fermentation medium is 5.5, and it is cultivated for 120 hours under the condition of 30 ℃ and 175r / min to obtain the fermentation liquid; Centrifuge to obtain the crude enzyme solution containing cellulase. After determination, the CMC enzyme activity of the crude enzyme solution of the strain is 24.52IU / ml, the FPA enzyme activity is 2.68IU / ml, and the β-glucosidase is 2.03IU / ml, which is compared with the enzymes produced by other existing strains (see table 4), the CMC enzyme activity of t...

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Abstract

The invention discloses a Eupenicillium javanicum bacterial strain and a screening method thereof. The preserving number of the bacterial strain is CCTCC M208204. The bacterial strain of the Eupenicillium javanicum can be used for producing cellulose, the step of which comprises that: the bacterial strain is inoculated into a seed culture medium and is cultured for 48-84 hours in the temperature of 25-32 DEG C so as to obtain the seed liquid; the seed liquid is inoculated into a fermentation medium according to 3-10% of inoculums concentration; the initial pH value of the fermentation medium is 4.5-6.0; the fermentation medium is cultured for 96-12 hours in the temperature of 25-32 DEG C so as to obtain fermentation liquor; the crude enzyme liquid containing cellulase is obtained by the centrifugation of the fermentation liquor. The cellulose produced by the Eupenicillium javanicum bacterial strain has the advantages of high activity, low cost, etc.

Description

technical field [0001] The invention relates to a strain of fungus and its screening and cultivation method and application, in particular to a strain producing high-activity cellulase and its screening and cultivation method and application. Background technique [0002] At present, cellulase has been widely used in various technical fields such as brewing, fruit juice and vegetable juice processing, grain processing, food, feed, papermaking, active ingredient extraction of Chinese herbal medicine, plant genetic engineering, cell engineering, and crude oil extraction. The purpose is to realize the decomposition and conversion of cellulose. The mechanism of cellulase is mainly to break the β-1,4-glucosidic bond connecting glucose molecules through hydrolysis, and finally decompose and convert cellulose into small molecular sugars. Currently, those skilled in the art generally believe that at least three active enzyme components with different functions but complementary effe...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/42C12Q1/04C12R1/645
Inventor 王义强陈介南刘海波何钢周再魁
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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