Method for separating anaerobic ammonia oxidizing bacteria

A kind of anammox bacteria, anaerobic ammonium oxidation technology, applied in the direction of bacteria, etc., can solve the problems of high grade requirements, not suitable for popularization, etc., and achieve the effect of simple steps

Inactive Publication Date: 2010-11-17
SOUNDGRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The use of density gradient centrifugation to isolate bacterial strains requires high levels of laboratories and should not be popularized

Method used

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Comparison scheme
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Embodiment 1

[0013] This embodiment provides a method for isolating anammox bacteria, which has the advantages of simplicity and speed compared to the existing separation of anammox bacteria by density gradient centrifugation. The method is specifically as follows:

[0014] Raw material collection: collect granular sludge as raw material, and make the granular sludge into a bacterial suspension; the granular sludge solution taken from the upflow anaerobic sludge bed can be used as raw material, and the granular sludge solution After the granular sludge is crushed, the bacterial suspension is made.

[0015] Primary screening enrichment culture: carry out primary screening enrichment culture on the prepared bacterial suspension to obtain primary screening culture; specifically, transfer the bacterial suspension into primary screening anaerobic ammonium oxidation enrichment medium, using Pyrogallic acid method, specifically, the pyrogallic acid method is: laying a layer of sterilized absorben...

Embodiment 2

[0029] This embodiment provides a method for isolating anammox bacteria, which has the advantages of simplicity and speed compared to the existing separation of anammox bacteria by density gradient centrifugation. The method is specifically as follows:

[0030] Step 1, collecting granular sludge as raw material: selecting the granular sludge solution in the upflow anaerobic sludge bed (UASB) as the separation source;

[0031] Step 2, processing the collected granular sludge raw materials, and ultrasonically crushing the granular sludge solution to make a bacterial suspension;

[0032] Step 3, primary screening enrichment culture: transfer the bacterial suspension prepared in step 2 into the primary screening anammox enrichment medium, which can be coated on the primary screening anammox enrichment medium by coating , yeast extract or glucose stock solution 0.5mg / L, using pyrogallic acid method, anaerobic culture at 25-35°C for 10-15 days, and the primary screening culture was ...

Embodiment 3

[0044] This embodiment provides a method for isolating anammox bacteria, which has the advantages of simplicity and speed compared to the existing separation of anammox bacteria by density gradient centrifugation. The method is specifically as follows:

[0045] Step 1, collecting granular sludge as raw material: selecting the granular sludge solution in the upflow anaerobic sludge bed (UASB) as the separation source;

[0046] Step 2, processing the collected raw materials, and ultrasonically crushing the granular sludge solution to make a bacterial suspension;

[0047] Step 3, primary screening enrichment culture: transfer the bacterial suspension prepared in step 2 to primary screening anammox enrichment medium, yeast extract or glucose stock solution (yeast extract or glucose 200mg / L) 1mg / L In the method, the pyrogallic acid method is used, and the anaerobic culture is carried out at a temperature of 25 to 35 ° C for 10 to 15 days, and the culture is obtained after the cultu...

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Abstract

The invention discloses a method for separating anaerobic ammonia oxidizing bacteria. The method comprises the following steps of: collecting a raw material, namely, collecting particle sludge as a raw material and preparing bacterial suspension by using the particle sludge; performing preliminary screening enrichment culture, namely, performing preliminary screening enrichment culture on the prepared bacterial suspension to obtain a preliminary screening culture; and performing secondary screening identification culture, namely, performing secondary screening identification culture on the culture obtained by the preliminary screening enrichment culture, detecting a culture obtained after the secondary screening identification culture and determining that strain of the culture is the anaerobic ammonia oxidizing bacterium if the culture passes the detection. The separation method has the advantages of separating the anaerobic ammonia oxidizing bacteria simply and rapidly, obtaining a pure culture of the anaerobic ammonia oxidizing bacteria and contributing to theoretical research of microbiology and industrial application.

Description

technical field [0001] The invention relates to a method for separating bacteria with anaerobic ammonia oxidation activity. Background technique [0002] Anaerobic ammonia oxidation (ANAMMOX) technology has been widely concerned by researchers in wastewater treatment due to its high efficiency, low consumption and environmental friendliness. Compared with the traditional nitrification-denitrification biological denitrification method, the anammox process has the following advantages: the anammox bacteria are autotrophic bacteria, and there is no need to add organic matter as an electron donor, which can save costs and prevent secondary denitrification. Pollution: In the anaerobic ammonium oxidation reaction, it is only necessary to oxidize ammonia to nitrite. Compared with the traditional nitrification-denitrification process, it is necessary to add acid and alkali agents, and the anaerobic ammonium oxidation reaction is a near-neutral reaction , the amount of biological ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 屈佳玉
Owner SOUNDGRP CO LTD
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