Dunaliella tertiolecta mutant strain with high growth rate and complex mutation breeding method thereof

Abstract

The invention relates to a method adopting ultraviolet mutation and high temperature and light to perform complex mutation on Dunaliella tertiolecta, which performs screening by using the growth rate of microalgae as an index under a normal culture condition to finally obtain a mutagenic strain ENN0001-1 with higher growth rate compared with a wild strain. After re-screening culture, the biomass (OD750) of the mutagenic strain is increased by 17.6 percent compared with the wild strain, and the chlorophyll content (OD680) is increased by 8 percent compared with the wild strain.

Description

Technical field

[0001] The present invention relates to the field of microbial engineering. Specifically, the present invention relates to a method for breeding Dunaliella using ultraviolet mutagenesis and high temperature and high light intensity treatment, and a Dunaliella mutagenic strain ENN0001- with high growth rate obtained by the method. 1. Background technique

[0002] Due to the declining fossil energy and the increasing environmental pollution caused by the use of fossil energy, researchers have focused their attention on the development and utilization of renewable energy. Among all kinds of renewable energy, the energy with wide practical value is biomass energy. Biomass is one of the most common renewable energy resources on the earth. It includes forestry biomass, energy crops, aquatic plants, agricultural waste, urban garbage, organic wastewater, and human and animal manure. In the recycling of biomass, the amount of carbon produced by biomass conversion is almost...

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