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Built-in light source bioreactor and microalgae culture method

A bioreactor, built-in light source technology, applied in photobioreactor, bioreactor/fermenter combination, bioreactor/fermenter for specific purposes, etc., can solve the problem of poor controllability, low efficiency and low degree of automation And other issues

Inactive Publication Date: 2014-09-03
LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the microalgae cultivation photoreactor in the prior art is compared with the direct cultivation in the open air such as track ponds and multi-stage ponds, the cultivation density and the yield per unit area are significantly improved, but the light source for microalgae growth and oil production is still natural light. The utilization of sunlight by microalgae is not sufficient, and it is uncertain and uncontrollable, which completely runs counter to the stable and continuous requirements of industrial production. In addition, auxiliary breeding functions such as nutritional supplementation and carbon dioxide inflation have not been automated, making it incompatible with actual production. There is still a long way to go for industrial application
[0005] Therefore, there is an urgent need for a technology in this field that can overcome the disadvantages of poor controllability, low degree of automation, and low efficiency of the microalgae cultivation photoreactor in the above-mentioned prior art.

Method used

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  • Built-in light source bioreactor and microalgae culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] A conventional freshwater species of Nannochloropsis limnetica was selected, the initial culture density was 0.1g / L, and the liquid level depth of the reactor was 80cm (the cross-sectional area was 1m×1.5m). In the first stage, a red light LED with a wavelength of 706nm was used as the light source, and the temperature was kept at 24°C. Trace elements such as ammonium phosphate, zinc, and iron were added to the culture medium in advance, and a certain amount of CO was injected into the culture medium. 2 (about 90%) and NO or NO as nitrogen source 2 After waiting for nitrogen oxides for one hour, put in the algae seeds according to the initial culture density, and start the culture. After 36 hours, it was recorded that the biomass density increased to 6.86g / L (dry weight / solution, the same below), and after 72 hours, the biomass density 9.71g / L was recorded, and the microalgae in the reactor was moved to another reactor ( The depth of the liquid level is 80cm, and the c...

Embodiment 2

[0119] The freshwater species Nannochloropsis limnetica was selected, the initial culture density was 0.1g / L, and the liquid level depth of the reactor was 80cm (the cross-sectional area was 1m×1.5m). In the first stage, a blue LED with a wavelength of 450nm was used as the light source, and the temperature was kept at 24°C. Trace elements such as ammonium phosphate, zinc, and iron were added to the culture medium in advance, and a certain amount of CO was injected into the culture medium. 2 (about 90%) and NO or NO as nitrogen source 2 After waiting for nitrogen oxides for one hour, put in the algae seeds according to the initial culture density, and start the culture. After 36 hours, it was recorded that the biomass density increased to 5.66g / L, and after 72 hours, it was recorded that the biomass density was 8.03g / L. 2m×3m), stop supplying nitrogen oxides and keep CO 2 Supply, using a red LED with a wavelength of 706nm as the light source, the temperature is constant at 3...

Embodiment 3

[0122] The freshwater species of Nannochloropsis limnetica was selected, the initial culture density was 0.1g / L, and the depth of the liquid level in the reactor was 80cm (the cross-sectional area was 2m×3m). In the first stage, a red light LED with a wavelength of 706nm was used as the light source, and the temperature was kept at 24°C. Trace elements such as ammonium phosphate, zinc, and iron were added to the culture medium in advance, and a certain amount of CO was injected into the culture medium. 2 (about 90%) and NO or NO as nitrogen source 2 After waiting for nitrogen oxides for one hour, put in the algae seeds according to the initial culture density, and start the culture. Samples were taken at a certain time to measure the biomass density. Breeding ends after 144 hours.

[0123] Results: After 36 hours, the measured biomass density increased to 6.86g / L, after 72 hours, the measured biomass density was 9.91g / L, after 108 hours, the measured biomass density was 12.2...

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Abstract

The invention discloses a built-in light source bioreactor. The built-in light source bioreactor comprises a reaction vessel, a material feed and discharge device, a light-emitting device, a nutrient distributing device and a gas distributor, wherein the reaction vessel is provided with a cover plate, and used for containing a culture solution for microalgae growth; the material feed and discharge device is connected with the reaction vessel in a seal mode and provided with a valve and a switch, and the microalgae are pumped in or out of the reaction vessel through the material feed and discharge device; the light-emitting device is used for generating a light source required by microalgae growth; the nutrient distributing device is used for supplying nutrients by microalgae growth into the reaction vessel; and the gas distributor is used for supplying gas required by microalgae growth into the reaction vessel. The built-in light source bioreactor is free from the influence of weather changes, can perform the microalgae reproduction by different stages, has high controllability, and is beneficial to implementing stable and continuous industrial production; and the bioreactor tandem and / or parallel system enhances the large-scale culture efficiency of microalgae.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a built-in light source bioreactor and a microalgae cultivation method. Background technique [0002] Microalgae biodiesel is the future development direction of liquid biofuel. It has high energy conversion efficiency. Compared with ordinary terrestrial crops, the yield per unit area can be dozens of times higher, so that industrial farming can be realized. The principle of oil production from microalgae is to use the photosynthesis of microalgae to convert the carbon dioxide produced in the chemical production process into the biomass of the microalgae itself to fix the carbon element, and then convert the carbon material of the microalgae itself into oil through an induced reaction. Then use physical or chemical methods to convert the oil in the microalgae cells to the outside of the cells, and then refine and process it to produce biodiesel, which can convert the nutrients and ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/04C12N1/12C12P7/64C12R1/89
CPCY02E50/13C12M21/02C12M23/58C12M29/06C12M31/08C12M31/10C12M41/12C12M41/26C12M41/36C12N1/12C12P7/649Y02E50/10
Inventor 张玟籍陈辉
Owner LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
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