Microalgae high-throughout culture equipment

A microalgae culture, high-throughput technology, applied in biochemical equipment and methods, microorganisms, microorganism-based methods, etc., can solve problems such as difficulty in achieving light and ventilation effects, unsatisfactory light intensity, and large heat production.

Active Publication Date: 2011-10-12
ENN SCI & TECH DEV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the laboratory system for high-throughput culture of algae species is relatively simple. The culture of algae strains is generally placed in an artificial climate box in a triangular flask system, with no ventilation or simply inserting a ventilation tube for ventilation, light and The temperature is regulated by the artificial climate box, and the temperature control is relatively uniform, but it is difficult to achieve consistent lighting and ventilation effects in this way
In addition, there is another culture system that is placed in a shaking table. Both the light and temperature are regulated by the shaking table, and the gas exchange is accelerated by shaking. Problems meeting the needs of algae growth and high-throughput screening
In addition, the above two methods also have the pro

Method used

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  • Microalgae high-throughout culture equipment

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Experimental program
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Effect test

Embodiment 1

[0052] In this embodiment, whether the light intensity distribution of the designed shaker meets the requirements of high-throughput screening of microalgae is evaluated by measuring the light intensity at different positions.

[0053] The experimental procedure is as follows: set the temperature of the shaker at 25°C and the light intensity at 4000 lux, and measure the light intensity at the upper left corner, upper right corner, lower left corner, lower right corner and center of the designed shaker with an illuminance meter. A 96-microwell plate was used as the benchmark for measurement.

[0054] Data processing uses SPSS Statistics 17.0 software. The uniformity of illumination among the wells of each microplate was evaluated by calculating the coefficient of variation (CV) of the illumination intensity at each well in each microplate and one-way analysis of variance (ANOVA). The uniformity of illumination at different positions was evaluated by calculating the coefficient...

Embodiment 2

[0058] In this embodiment, 96-well plates, 48-well plates, 24-well plates, and 12-well plates are respectively selected, and the lighting device in the present invention is used to provide light, and the algae strain Chlorella vulgaris (purchased from the Institute of Hydrobiology, Chinese Academy of Sciences, freshwater algae species Library, No. 1068) for cultivation, using BG11 medium (see Table 1-3 below).

[0059] Table 1 Composition and content of BG11 medium

[0060] serial number

Element

Volume ( / L)

1

NaNO 3

1.5g

2

MgSO 4 ·7H 2 o

0.075g

3

CaCl 2 2H 2 o

0.036g

4

Citric acid

0.006g

5

Fe-EDTA stock solution

1mL

6

Na 2 CO 3

0.02g

7

A5 Trace element

1.0ml

[0061] Table 2A 5 Trace element composition and content

[0062] serial number

Element

Quantity (g / L)

1

h 3 BO 3

2.86

...

Embodiment 3

[0071] In the embodiment, the correlation between the miniaturized culture of algae cells using the high-throughput culture equipment of the present invention and conventional culture methods will be analyzed. Under certain experimental conditions, the comparison of the growth ability of three different algae species in shake flasks and microwell plates is used to evaluate the use of high-throughput culture equipment of the present invention to cultivate microalgae through microwell plates and culture with shake flasks Whether there is a high correlation between microalgae.

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Abstract

The invention provides microalgae high-throughout culture equipment comprising a frame, an oscillating mechanism, a temperature control device, a humidity control device, a ventilation control device, an objective table and a lighting device, wherein the objective table is suitable for fixing a culturing structure for containing an algae seed or algae liquid, the lighting device is arranged belowthe objective table and is suitable for providing even lighting for microalgae culture, the lighting device consists of a light source and a light guiding structure which comprises a light guiding layer, light reflection structures are arranged on the side surfaces and bottom surface of the light guiding layer so as to guide light from the light source to emit out from one surface of the light guide layer, therefore, light from the light source is evenly provided to the culturing structure containing the algae seed or algae liquid, and the lighting uniformity degree and lighting intensity formicroalgae high-throughout culture can be improved so as to meet the demands of microalgae growth and high-throughout screening.

Description

technical field [0001] The invention relates to the field of industrial application of microalgae, and in particular relates to equipment suitable for high-throughput cultivation of microalgae used in high-throughput screening of algae species. Background technique [0002] Microalgae are a kind of autotrophic plants widely distributed in land and ocean, rich in nutrients and high in photosynthetic utilization. Many microalgae can accumulate a large amount of oil in their cells. Some algae can contain up to 80% of the dry weight of the cell in the absence of nitrogen. Oil can be extracted from this type of microalgae by biorefinery for food preparation. oil and biodiesel; microalgae can also be used in industrial processes such as wastewater treatment to reduce environmental pollution. [0003] The environmental friendliness of microalgae, its health benefits to humans and animals, and its good application of bioenergy have triggered a global upsurge in research and develop...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/00C12R1/89
CPCC12M21/02C12M27/16C12M31/08C12M41/14
Inventor 吴洪王媛媛郝玉友袁旭军张明张引峰刘峰赵正群
Owner ENN SCI & TECH DEV
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