Method and apparatus for producing lipids through microalgae culture

A microalgae cultivation and microalgae technology, applied in the field of biofuel preparation, can solve the problems not involved in the photosynthesis of microalgae and the monitoring of lipid production and separation process, and achieve low capital investment and operation costs, occupying an area of Effect of small, high lipid concentrations

Inactive Publication Date: 2014-03-12
SIEMENS AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] The above schemes can improve the lipid yield of microalgae to a certain extent, but none of them involve the monitoring of photosynthesis and lipid production and separation process of microalgae

Method used

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  • Method and apparatus for producing lipids through microalgae culture
  • Method and apparatus for producing lipids through microalgae culture
  • Method and apparatus for producing lipids through microalgae culture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1. The parameter design of culture system

[0067] Controlled variables (CVs) can be selected from cell concentrations in photoautotrophic and photoheterotrophic reactors, lipid concentrations in photoheterotrophic reactors, and nutrient (preferably N) concentrations in photoheterotrophic reactors. In the example of the present invention, the system had a lipid yield of 30 tons / ha-year, corresponding to a dry weight yield of microalgae of 60 tons / ha-year. Table 1 summarizes the parameters and nominal operating points for this method. For ease of collection and downstream processing, 2kg / m is preferred 3 cell concentration and 50% lipid concentration.

[0068] Table 1. Design parameters of the culture system

[0069] parameters

Embodiment 2

[0070] Embodiment 2. Determination of the best culture point

[0071] The optimal culture point is dependent on the specific microalgae species and can be determined from batch culture experiments for lipid production. Examples of the present invention use Chlorella vulgaris. The first stage of cultivation is carried out photoautotrophically in a photoautotrophic reactor, ideally at the optimal operating point; the second stage of cultivation is carried out in a photoheterotrophic reactor at its optimal operating point at different carried out in the way of raising. This approach achieves high growth rates and high lipid concentrations of the microalgae. The control system of the present invention ensures that the cultivation process runs under optimal conditions without external interference.

[0072] Figure 4 For microalgae growth and lipid accumulation curves in batch mode, arrows indicate the optimal operating point for continuous culture. Such as Figure 4 It was s...

Embodiment 3

[0073] Embodiment 3. Method model and computer simulation

[0074] Process models and computer simulations aid in process development and control system design. The microalgae cultivation method and control system proposed by the present invention can be verified by computer simulation. Some model parameters were obtained from laboratory results of the freshwater green alga Chlorella vulgaris FACHB-31 (FACHB-Collection). In BG11 medium, sodium nitrate (nitrogen source) was chosen as the limiting nutrient, while other nutrients were in excess. Table 1 lists the design parameters of the culture system.

[0075] During the simulation, it is preferable to have a constant culture volume V 1 The photoautotrophic reactor operates as a chemostat. Input and output have the same volumetric flow rate F 1 , get the dilution ratio D=F 1 / V 1 .

[0076] d X 1 dt = ...

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Abstract

A purpose of the present invention is to provide an apparatus for producing lipids through microalgae culture. The apparatus comprises: at least a photoautotrophy reactor, wherein the photoautotrophy reactor is positioned on the downstream of a tank, is communicated with the tank, and is provided for photoautotrophy growth of microalgae; at least a photoheterotrophy reactor, wherein the photoheterotrophy reactor is positioned on the downstream of the photoautotrophy reactor, is communicated with the photoautotrophy reactor, and is provided for photoheterotrophy growth of microalgae and accumulation of lipids; and a microalgae concentration control system, wherein the microalgae concentration control system controls a dilution rate of the photoautotrophy reactor through monitoring the microalgae concentration in the photoheterotrophy reactor. The present invention further provides a corresponding method. With the apparatus and the method, high microalgae growth speed and high lipid concentration can be achieved, the occupation area is small, the capital investment is low, and the operation cost is low.

Description

technical field [0001] The present invention relates to the production of biofuels. In particular, the present invention relates to methods and devices for the production of lipids by microalgal culture. Background technique [0002] With rising oil prices and global warming caused by greenhouse gas emissions, academia and industry are increasingly focusing on alternative fuels and carbon dioxide utilization technologies. Single-celled microalgae organisms can use photosynthesis to convert carbon dioxide into biomass. These algae can be cultivated, harvested and processed to obtain lipids that can be refined into biofuels such as diesel. Therefore, the large-scale cultivation and processing of algae is one of the effective measures to solve environmental and energy problems. [0003] The principle process of lipid production by microalgae mainly includes: the microalgae uses photosynthesis to convert carbon dioxide in the air into the biomass of the microalgae itself, ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/36C12P7/64C12R1/89
CPCY02E50/13C12M21/02C12M23/58C12M41/32C12M41/36C12M41/38C12M41/44C12M47/06C12P7/6463Y02E50/10
Inventor 范顺杰孟显涛
Owner SIEMENS AG
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