Method for promoting microalgae growth and grease accumulation in BG-11 culture medium

A technology of BG-11 and oil accumulation, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as environmental pollution and resource waste, achieve environmental protection, solve environmental problems, and increase biomass production rate and the effect of oil yield

Inactive Publication Date: 2019-09-10
KUNMING UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional treatment method for walnut shells is to prepare activated carbon or packed columns, but usually, this solid waste gas is burned directly, which not only causes a waste of resources, but also produces a large amount of greenhouse gases, causing environmental pollution

Method used

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  • Method for promoting microalgae growth and grease accumulation in BG-11 culture medium
  • Method for promoting microalgae growth and grease accumulation in BG-11 culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of medium: first prepare BG-11 medium, adjust the pH value between 6.8-7.0 with HCl solution, sterilize at 121°C for 20 minutes; add water 9.5 times the mass of walnut shells, and store at 95°C Incubate for 240 min, adjust the pH value between 6.8-7.0 with NaOH solution, obtain walnut shell extract with a walnut shell extract concentration of 100 g / L, subpackage, and sterilize at 121°C for 20 min; mix the walnut shell extract with BG- 11 was mixed in the reactor, and the concentration of walnut shell extract in the medium after mixing with BG-11 was 20g / L.

[0026] (2) Cultivation of microalgae: insert oil-producing microalgae into the above-mentioned prepared medium, and pass in CO 2 -Air mixed gas, light culture, the initial inoculum volume is 0.4 g / L, the light intensity is 6500 lux, and CO is introduced 2 - CO in the air mixture 2 The content is 12%, and the culture temperature is 25±1℃.

[0027] (3) Extraction of oil from microalgae: culture the ...

Embodiment 2

[0030] (1) Preparation of medium: first prepare BG-11 medium, adjust the pH value between 6.8-7.0 with HCl, and sterilize at 121 °C for 20 minutes. Add water with 9.5 times the mass of walnut shells, incubate at 95°C for 240 minutes, adjust the pH value between 6.8-7.0 with NaOH solution, and obtain walnut shell extracts with a walnut shell extract concentration of 100 g / L. Sterilize at ℃ for 20 min. The walnut shell extract and BG-11 were mixed in the reactor, and the concentration of the walnut shell extract in the medium after mixing with BG-11 was 30g / L.

[0031] (2) Cultivation of microalgae: insert oil-producing microalgae into the above-mentioned prepared medium, and pass in CO 2 - Air mixed gas, light culture, the initial inoculum volume is 0.4 g / L, the light intensity is 6700lux, and CO is introduced 2 - CO in the air mixture 2 The content is 12%, and the culture temperature is 25±1℃.

[0032] (3) Extraction of oil from microalgae: culture the microalgae to the la...

Embodiment 3

[0035] (1) Preparation of medium: first prepare BG-11 medium, adjust the pH value between 6.8-7.0 with HCl solution, and sterilize at 121 °C for 20 min. Add water with 9.5 times the mass of walnut shells, incubate at 95°C for 240 minutes, adjust the pH value between 6.8-7.0 with NaOH solution, and obtain walnut shell extracts with a walnut shell extract concentration of 100 g / L. Sterilize at ℃ for 20 min. The walnut shell extract and BG-11 were mixed in the reactor, and the concentration of the walnut shell extract in the medium after mixing with BG-11 was 40g / L.

[0036] (2) Cultivation of microalgae: insert oil-producing microalgae into the above-mentioned prepared medium, and pass in CO 2-Air mixed gas, light culture, the initial inoculation amount is 0.4 g / L, the light intensity is 7000 lux, and CO is introduced 2 - CO in the air mixture 2 The content is 12%, and the culture temperature is 25±1℃.

[0037] (3) Extraction of oil from microalgae: culture the microalgae to...

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Abstract

The invention discloses a method for promoting microalgae growth and grease accumulation in a BG-11 culture medium, and belongs to the technical field of production of biodiesel through microalgae. According to the method, the BG-11 culture medium serves as a basic culture medium for microalgae culture, then a walnut shell extract solution culture medium is prepared, the pH is adjusted to 6.8-7.0,and the two culture mediums are sterilized at 121 DEG C for 20 minutes separately and then mixed and loaded into a photobioreactor. Oil-producing microalgae is inoculated for culture, after the microalgae grows to a stable period, algae cells are collected by centrifugation, and a chloroform / methanol (2:1, v / v) solution is utilized for extracting grease in the algae cells. The method is easy andfeasible to operate, nutrients in walnut shells can be effectively utilized for increasing the biomass and grease content of the microalgae, the production cost is reduced, and the generation of greenhouse gas is reduced.

Description

technical field [0001] The invention relates to a method for promoting the growth of microalgae and oil accumulation in BG-11 culture medium, and belongs to the technical field of biodiesel production by using microalgae. Background technique [0002] As a substitute for fossil fuels, biodiesel has many advantages. In recent years, more and more people have begun to pay attention to the field of biodiesel production from microalgae. However, in the process of using microalgae to prepare biodiesel, the development of its industrialization is limited due to the high cost of microalgae culture, low biomass yield and oil yield. Cultivating microalgae by combining waste with carbon dioxide is an effective method to reduce the cost of microalgae culture and increase the yield of biomass and oil. For example, when 2.5% carbon dioxide is introduced into domestic sewage, Scenedesmus Scenedsumis The biomass of sp. reached 1.37 g / L, and the oil content was 33.3% of the dry cell wei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C11B1/10C12R1/89
CPCC11B1/10C12N1/12C12P7/649Y02E50/10
Inventor 韩本勇乔腾生余旭亚宁德鲁耿树香马婷
Owner KUNMING UNIV OF SCI & TECH
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