Freshwater eukaryoticmicroalgae culture method

A cultivation method and technology for microalgae, applied in biochemical equipment and methods, unicellular algae, enzymology/microbiology devices, etc., can solve the problem of poor controllability, uncontrollability, insufficient utilization of sunlight by microalgae, etc. question

Inactive Publication Date: 2016-02-10
SHANGHAI XIMING BIOTECH CO LTD
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  • Claims
  • Application Information

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Problems solved by technology

Although the microalgae cultivation photoreactor in the prior art is compared with the direct cultivation in the open air such as raceway ponds and multi-stage ponds, the cultivation density and the output per unit area are significantly improved, but due to the growth of microalgae and the directional induction of effective substances, the The light source is still natural light, and the use of sunlight by microalgae is not sufficient, which is uncertain and uncontrollable, completely contrary to the sta

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  • Freshwater eukaryoticmicroalgae culture method
  • Freshwater eukaryoticmicroalgae culture method
  • Freshwater eukaryoticmicroalgae culture method

Examples

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Embodiment 1

[0127] Propagate microalgae in a small artificial light source test box, the selected algae species is Chlorella pyrenoidosa, and the inoculation concentration is 0.3g / L (dry weight). The artificial light source is a three-color LED light source, the wavelengths are 650±5nm, 440±5nm, 720±10nm, the ratio of the three-color light source is 10:3:2, the light intensity at the darkest part is about 3000lx, and the light and dark alternate time is 10 hours (bright): 2 hours (dark), the dark light power is about 40% of the bright light power. Introduce carbon dioxide, the pressure is 0.4Mpa, the pore diameter of the released ceramic membrane pressure tube is 10nm, the mixing ratio of carbon dioxide and air is 2%-3%, and the ventilation is continued until PH=6.5 to stop, reducing the mixing ratio of carbon dioxide and air , until the pH interval remains between 6.5-6.8. Breeding temperature is maintained at 27 degrees ± 1 degree.

[0128] The nutrient selection of culture fluid in t...

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Abstract

The invention discloses a freshwater eukaryoticmicroalgae culture method. The method includes steps: (1), providing culture equipment used for culturing freshwater eukaryoticmicroalgae, wherein the culture equipment comprises a reaction container, a light emitting device, a nutrition distributing device and a gas supply device, the reaction container is used for containing a culture solution for microalgae growth, the light emitting device provides light needed for microalgae growth in the reaction container, the nutrition distributing device is used for providing nutrients needed for microalgae growth and reproduction and can provide manganese ions for the culture solution, and the gas supply device is used for providing gas needed for microalgae growth in the reaction container; (2), culturing the freshwater eukaryotic microalgae in the culture equipment. An infrared light source is adopted in the method, and a proper amount of manganese ions are provided into and a proper amount of carbon dioxide is fed into the culture solution, so that microalgae culture efficiency is improved greatly.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for cultivating freshwater eukaryotic microalgae. Background technique [0002] Microalgae biomass is the future development direction of biomass utilization. It has the characteristics of high energy conversion efficiency. Compared with ordinary terrestrial crops, the yield per unit area can be dozens of times higher, so that industrial farming can be realized. The basic principle of microalgae growth is to use microalgae photosynthesis to convert carbon dioxide into microalgae's own biomass to fix carbon elements, and synthesize oil, protein, polysaccharide, cellulose and other biomass through microalgae's own characteristics and functions . Use physical or chemical methods to transform the useful substances in microalgae cells to the outside of the cells, and then refine and process them to produce biodiesel, algae polysaccharides and other products. Therefore, ...

Claims

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Application Information

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IPC IPC(8): C12N1/12
CPCC12M1/04C12M1/34C12M1/36C12M1/38C12N1/12
Inventor 张玟籍陈辉
Owner SHANGHAI XIMING BIOTECH CO LTD
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