Method for breeding precious cross bred orchid

A technology of hybrid breeding and orchids, applied in the direction of botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of aseptic germination that have not been reported

Inactive Publication Date: 2006-02-08
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although someone has done research on the breeding of Cmbidium plants and the germination of seeds,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Breeding and propagation of COt-1

[0031] 1. Source of materials: Two parent orchid strains, Cmbidium tracyanum Lcastle var huang su and C. mastersii Griff, were collected from the mountainous area of ​​Yunnan. The female parent and C. mastersii Griff were the male parent. ;

[0032] 2. Training method:

[0033] (1) Hybrid breeding

[0034] 1) Establish an orchid resource garden, the environment in the resource garden can be manually regulated to create the best environment most suitable for the growth and development of orchids;

[0035] 2) The natural flowering periods of these orchids are close to the same, and the breeding operation is carried out when 1 to 2 flower buds at the base of the inflorescence of the parents are open;

[0036] 3) Breeding operation: wash your hands and tweezers, gently remove the parent herb cap (anther top cap) with your hands, use the tweezers to pick off the pollen block of the male parent, and send it into the cavity (st...

Embodiment 2

[0044] Example 2: Breeding and propagation of COt-2

[0045] Male parent: Cmbidium tracyanum L castle var huang su, female parent: C.mastersii Griff),

[0046] (1) hybrid breeding is the same as embodiment 1;

[0047] (2) Aseptic germination of seeds

[0048] 1) explant treatment: same as embodiment 1;

[0049] 2) Seed germination medium: The improved MS (i.e. 1 / 2MS) is selected as a more suitable basic medium, and the formula with growth regulator is: 1 / 2MS+6-BA2.0mg / L+NAA0.5mg / L (cytokinins and auxins);

[0050] 3) culture condition: same as embodiment 1;

[0051] 4) After the seeds germinate, they will first sprout, then grow young roots, and transfer to the improved MS+KT2.5mgL -1 +NAA0.2mg.L -1 Cultured in the culture medium, after 4 to 6 months, it gradually becomes a test-tube plantlet with a root system and can be hardened and transplanted.

Embodiment 3

[0052] Example 3: Breeding and propagation of COi-1

[0053] Male parent: C. mastersii Griff, female parent: Cicada orchid (C. iridioides), the breeding and propagation methods are the same as those in Example 1 or Example 2.

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Abstract

The invention relates to a method of breeding the rare hybrid orchid, which is characterized in: crossbreeding and fruit cultivating the gathered Cmbidium tracyanum L castle var huang su, C. mastersii Griff and C. iridioides in the resource garden, and the hybrid orchid is got by crossbreeding, the seed sterile germination, the explant treating, seed germination, young root emerging and switching culture, etc. The applying of Cmbidium tracyanum L castle var huang su, C. mastersii Griff and C. iridioides as parental generation for crossbreeding, which can break a series of technique bottleneck, has not been reported. The method cannot only breed new strain but also can precede rapid breeding, which can meet the industrialization demand.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a breeding and propagation method of precious hybrid orchids. Background technique [0002] Orchid hybrid seeds are small, contain some immature embryos, no endosperm, and it is difficult to germinate in a natural state. Although the research on the breeding of Cmbidium plants and the germination of seeds has been done, there are no reports on the hybrid breeding and seed germination of the above hybrid Cmbidium plants. Contents of the invention [0003] The purpose of the present invention is to provide a breeding and propagation method of precious hybrid orchids which lays an important technical foundation for orchid breeding and industrial production. [0004] The invention studies the aseptic germination of four kinds of orchid seeds, and successfully cultivates a large number of test-tube seedlings. [0005] Cmbidium tracyanum L castle var huang su, C. mstersii...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 李枝林余朝秀王卜琼王玉英黄丽萍
Owner YUNNAN AGRICULTURAL UNIVERSITY
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