Bioerosion action method of quantitative microalgae to limestone

A bio-dissolution, limestone technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effects of reliable data, mature technology, and simple operation

Active Publication Date: 2013-06-26
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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  • Abstract
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Problems solved by technology

[0006] At present, there is no report on the method of quantifying the bioerosion effect of microalgae on limestone, and the quantification of microalgae bioerosion on limestone is extremely important. It can effectively evaluate the effect of microalgae on lakes and rivers on the ecological environment of karst areas and buildings such as dams. hazards, so as to achieve the purpose of predicting disasters

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  • Bioerosion action method of quantitative microalgae to limestone
  • Bioerosion action method of quantitative microalgae to limestone
  • Bioerosion action method of quantitative microalgae to limestone

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Embodiment Construction

[0028] Embodiments of the present invention: the first step is to measure the calcium-magnesium mass ratio of the limestone powder to be added, and add excessive limestone powder (less than 60 mesh) and no After adding the limestone powder, cultivate the microalgae to be tested, and then only add the limestone powder with a mass ratio of calcium to magnesium greater than 40 in another group of culture solution without adding algae;

[0029] In the second step, the microalgae to be tested are cultured under the culture conditions under investigation, and after different culture times, the chlorophyll a concentration CHLa of the culture solution added with limestone powder and cultured with algal bodies is respectively measured. i , Magnesium ion concentration CMg corresponding to the culture time solution i and the magnesium ion concentration of the solution in the culture solution that only added limestone powder and did not cultivate algae i , the initial concentration of ma...

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Abstract

The invention discloses a bioerosion action method of quantitative microalgae to limestone. One part of magnesium ions which are dissolved out of limestone are absorbed and utilized by the algae, and one part can cause the changes in concentration of the magnesium ions in a solution; the integration of a microalgae growth equation to time represents the bioerosion accumulative action time of the algae in unit volume of culture solution; an equation of the dissolving-out quantity of the microalgae in the unit volume of the culture solution to limestone magnesium along with the changes in bioerosion accumulative action time of the algae represents the dissolving-out quantity of the microalgae in the unit volume of the culture solution to limestone magnesium under different bioerosion accumulative action times of the algae, and then derivation is performed on the equation to represent the dissolving-out quantity of algae in unit biomass unit time to limestone magnesium; and the dissolving-out of magnesium can also directly reflect the erosion of limestone, so that the dissolving-out quantity of the algae in the unit biomass unit time to limestone magnesium can quantitatively reflect the bioerosion action of the microalgae to limestone. The bioerosion action method disclosed by the invention can fill in the blank of bioerosion action of the quantitative microalgae to limestone and has the advantages of mature technology, simplicity in operation and low cost.

Description

technical field [0001] The invention relates to a method for quantifying the biological dissolution of microalgae on limestone, and belongs to the fields of ecological environment control monitoring and engineering geological monitoring. Background technique [0002] The dissolution of limestone includes physical, chemical and biological effects. Limestone is often subjected to dissolution by surrounding organisms, so bioerosion is critical. There are abundant algae on the rock surface in karst areas, and a large number of algae in the water body erode the rocks in the basement and embankment. [0003] Although some people have studied the micromorphology and water holding capacity of biokarst formed by the dissolution of algae, lichens and mosses on the shallow surface of limestone, others have studied the role of microorganisms and carbonic anhydrase in the process of karstification. However, the quantitative research on the complex process of "biological erosion of lime...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/64
Inventor 吴沿友谢腾祥吴明津李海涛刘莹刘丛强王宝利
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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