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Method for biologically dissolving limestone by quantitative microalgae

A bio-dissolution, limestone technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of simple operation, low cost, and mature technology

Inactive Publication Date: 2013-02-06
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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Problems solved by technology

[0006] At present, there is no report on the method of quantifying the bioerosion effect of microalgae on limestone, and the quantification of microalgae bioerosion on limestone is extremely important. It can effectively evaluate the effect of microalgae on lakes and rivers on the ecological environment of karst areas and buildings such as dams. hazards, so as to achieve the purpose of predicting disasters

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  • Method for biologically dissolving limestone by quantitative microalgae
  • Method for biologically dissolving limestone by quantitative microalgae
  • Method for biologically dissolving limestone by quantitative microalgae

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Embodiment Construction

[0028] Embodiments of the present invention: the first step is to measure the calcium-magnesium mass ratio of the limestone powder to be added, and add excessive limestone powder (less than 60 mesh) and no After adding the limestone powder, cultivate the microalgae to be tested, and then only add the limestone powder with a mass ratio of calcium to magnesium greater than 40 in another group of culture solution without adding algae;

[0029] In the second step, the microalgae to be tested are cultured under the culture conditions under investigation, and after different culture times, the chlorophyll a concentration CHLa of the culture solution added with limestone powder and cultured with algal bodies is respectively measured. i , Magnesium ion concentration CMg corresponding to the culture time solution i and the magnesium ion concentration of the solution in the culture solution that only added limestone powder and did not cultivate algae i , the initial concentration of ma...

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Abstract

The invention discloses a method for biologically dissolving limestone by quantitative microalgae. A part of magnesium ions dissolved out from limestone are absorbed and utilized by the microalgae, and a part of magnesium ions are used for changing the concentration of magnesium ions in the solution; a biological growth rate equation multiplied by a time integral of time represents the microalgae biological dissolution accumulation time in a culture solution per unit volume; the equation that the dissolution quantity of limestone magnesium by microalgae in the culture solution per unit volume varies along with the microalgae biological dissolution accumulation time represents the dissolution quantity of limestone magnesium by microalgae in the culture solution per unit volume under different microalgae biological dissolution accumulation times, and the derivation of the equation represents the dissolution quantity of limestone magnesium by microalgae per unit biomass and unit time; and the dissolution of magnesium also directly reflects the dissolution of limestone, so that the dissolution quantity of limestone magnesium by microalgae per unit biomass and unit time can quantitatively reflect the biological dissolution of limestone by microalgae. The invention fills the blank for biologically dissolving limestone by quantitative microalgae, and the method has mature technologies, easiness in operation and low cost.

Description

technical field [0001] The invention relates to a method for quantifying the biological dissolution of microalgae on limestone, and belongs to the fields of ecological environment control monitoring and engineering geological monitoring. Background technique [0002] The dissolution of limestone includes physical, chemical and biological effects. Limestone is often subjected to dissolution by surrounding organisms, so bioerosion is critical. There are abundant algae on the rock surface in karst areas, and a large number of algae in the water body erode the rocks in the basement and embankment. [0003] Although some people have studied the micromorphology and water holding capacity of biokarst formed by the dissolution of algae, lichens and mosses on the shallow surface of limestone, others have studied the role of microorganisms and carbonic anhydrase in the process of karstification. However, the quantitative research on the complex process of "biological erosion of lime...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/64C12R1/89
Inventor 吴沿友谢腾祥吴明津李海涛刘莹刘丛强王宝利
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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