Culture method of microalgae

A cultivation method and microalgae technology are applied in the field of microalgae cultivation for inhibiting miscellaneous bacteria, can solve the problems of affecting the yield of microalgae, difficult to recover, etc., and achieve the effects of low cost, avoiding inhibition, avoiding and killing miscellaneous bacteria

Active Publication Date: 2014-05-07
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method adjusts the pH value of the microalgae culture system according to the different acid-base tolerance levels of the harmful organisms. The purpose of adjusting the pH value is to kill the harmful organisms, not to obtain tolerant microalgae; and some algae The tolerance of microalgae is not as strong as that of harmful organisms. While killing harmful organisms, the growth of microalgae is also greatly affected. For some algae species that are not strong in tolerance, after adjusting to a suitable pH value Growth may be difficult to restore, affecting microalgal yields

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The chlorella solution preserved in the laboratory was inserted into a 500mL shake flask containing 200mL SE medium at a volume ratio of 1:9 for autotrophic culture. The culture was carried out in a light-oscillating constant-temperature shaker. The sodium hydroxide was adjusted to 8.0, the shaker speed was 120rpm, the culture temperature was 25°C, and the light intensity was 3000lux; the pH value was increased step by step for 5 times, each time the pH was increased by 0.6, and each time the pH was increased, it was cultivated for 36 hours under the same conditions, and the domestication culture When the pH is 11.0, the algae solution tolerant to high pH is obtained. After step-by-step domestication and cultivation, some microalgae that cannot tolerate high pH have died, and the microalgae that continue to grow are microalgae that can tolerate high pH. They are used as seed liquid and inserted into 15L of heterotrophic medium. Heterotrophic culture was carried out in t...

Embodiment 2

[0022] Put the botrytis liquid preserved in the laboratory into a 500mL shake flask containing 200mL BG-11 medium at a volume ratio of 1:6 for autotrophic culture. The culture is carried out in a light-oscillating constant-temperature shaker. Use sodium hydroxide to adjust to 9.0, the shaker speed is 150rpm, the culture temperature is 30°C, and the light intensity is 3000lux; the pH value is increased step by step for 2 times, each time the pH is increased by 1.5, and each increase is cultivated under the same conditions for 60h. Cultivate until the pH is 12.00 to obtain algae liquid that tolerates high pH. After step-by-step domestication and cultivation, some microalgae that cannot tolerate high pH have died, and the microalgae that continue to grow are microalgae that can tolerate high pH. They are used as seed liquid and inserted into 15L of heterotrophic medium. Heterotrophic culture was carried out in the fermenter, the inoculation ratio was 1:6, the fermentation volume ...

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Abstract

The invention discloses a culture method of microalgae. The method comprises the following steps: (1) conducting domestication and culture on an original seed liquid of microalgae in autotrophic growth conditions by using a mode of increasing the pH value step by step, wherein the initial pH value is 8.0-9.0, which is increased by 2-8 times with pH increasing by 0.5-2 every time; and culturing the microalgae under the same conditions for 24-96 h after each time of pH value increasing, and domesticating and culturing to reach a pH value of 11.0-12.0, so as to obtain a microalgae liquid with high pH tolerance; (2) inoculating the domesticated microalgae liquid to a heterotrophic medium with pH value of 11.0-12.0 and cultivating for 10-24 h, then adjusting the pH vale of the culture medium to 7.0-8.0 and carrying out further culture; and (3) when the microalgae growth achieves a stable period, stopping the cultivation and harvesting microalgae cells. The method can effectively inhibit the growth of infectious microbes in microalgae culture, does not require addition of antibiotics, does not have adverse influences on the quality of microalgae, and is safe, reliable and low in price.

Description

technical field [0001] The invention belongs to the technical field of microalgae biology, and in particular relates to a microalgae cultivation method for inhibiting miscellaneous bacteria. Background technique [0002] Microalgae refers to tiny algae, which are microorganisms widely distributed on land, oceans, and rivers. The polysaccharides, proteins, and pigments produced by cell metabolism make them very good in the fields of food, medicine, genetic engineering, and liquid fuels. Development prospects. Many microalgae can not only grow autotrophically under light energy, but also grow heterotrophically using organic matter like bacteria and yeast under no light conditions. [0003] Microalgae can use light energy and inorganic carbon sources in nature for autotrophic growth, but autotrophic growth has the disadvantages of low growth rate, low final biomass, and long growth cycle. At the same time, microalgae can use organic matter as carbon and nitrogen sources for h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 师文静廖莎王领民张霖李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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