Culture method of microalgae
A cultivation method and microalgae technology are applied in the field of microalgae cultivation for inhibiting miscellaneous bacteria, can solve the problems of affecting the yield of microalgae, difficult to recover, etc., and achieve the effects of low cost, avoiding inhibition, avoiding and killing miscellaneous bacteria
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Embodiment 1
[0017] The chlorella solution preserved in the laboratory was inserted into a 500mL shake flask containing 200mL SE medium at a volume ratio of 1:9 for autotrophic culture. The culture was carried out in a light-oscillating constant-temperature shaker. The sodium hydroxide was adjusted to 8.0, the shaker speed was 120rpm, the culture temperature was 25°C, and the light intensity was 3000lux; the pH value was increased step by step for 5 times, each time the pH was increased by 0.6, and each time the pH was increased, it was cultivated for 36 hours under the same conditions, and the domestication culture When the pH is 11.0, the algae solution tolerant to high pH is obtained. After step-by-step domestication and cultivation, some microalgae that cannot tolerate high pH have died, and the microalgae that continue to grow are microalgae that can tolerate high pH. They are used as seed liquid and inserted into 15L of heterotrophic medium. Heterotrophic culture was carried out in t...
Embodiment 2
[0022] Put the botrytis liquid preserved in the laboratory into a 500mL shake flask containing 200mL BG-11 medium at a volume ratio of 1:6 for autotrophic culture. The culture is carried out in a light-oscillating constant-temperature shaker. Use sodium hydroxide to adjust to 9.0, the shaker speed is 150rpm, the culture temperature is 30°C, and the light intensity is 3000lux; the pH value is increased step by step for 2 times, each time the pH is increased by 1.5, and each increase is cultivated under the same conditions for 60h. Cultivate until the pH is 12.00 to obtain algae liquid that tolerates high pH. After step-by-step domestication and cultivation, some microalgae that cannot tolerate high pH have died, and the microalgae that continue to grow are microalgae that can tolerate high pH. They are used as seed liquid and inserted into 15L of heterotrophic medium. Heterotrophic culture was carried out in the fermenter, the inoculation ratio was 1:6, the fermentation volume ...
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