Method for culturing microalgae by using alcohol wastewater and fermentation exhaust

An alcoholic wastewater and alcoholic fermentation technology, applied in the field of bioengineering, can solve the problems of consuming large amounts of energy and increasing the cost of alcohol production.

Inactive Publication Date: 2015-04-29
河南天冠生物燃料工程技术有限公司
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Problems solved by technology

Due to the large amount of alcohol wastewater, the process, especially the aerobic aeration sta

Method used

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  • Method for culturing microalgae by using alcohol wastewater and fermentation exhaust

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Embodiment

[0012] Take 300L of two-stage anaerobic digestion alcohol wastewater from a factory (the total ammonia nitrogen content is 911.5mg / L, and the total phosphorus content is 88.3mg / L) and add it to the bioreactor, inoculate 5% microalgae cells, and wait 20 days for the microalgae cells to When the reproduction is vigorous, the total ammonia nitrogen and total phosphorus in the wastewater are determined to be 10.3mg / L and 0.35mg / L respectively, reaching the reuse standard. Filter and separate 3000g (dry weight) of the microalgae cell product, in which the bio-oil content is 40%. The purified reused water is directly added to the mixing process of alcoholic fermentation, and the alcohol concentration in the final fermentation liquid is equivalent to that of the control group (non-reused water).

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Abstract

The invention provides a method for culturing microalgae by using alcohol wastewater and fermentation exhaust, which comprises the steps of anaerobic digestion of alcohol wastewater, microalgae culture, wastewater recovered mixture for alcohol fermentation, and the like. The method specifically comprises the following steps: carrying out anaerobic digestion on the alcohol wastewater; culturing the microalgae by using the liquid obtained by solid-liquid separation to obtain a microalgae thallus product or extracted biological grease, wherein fermentation exhaust is introduced to supplement the carbon source required by microalgae growth in the process; and recovering the microalgae purification liquid to the alcohol production mixture technique. The method for culturing microalgae by using the alcohol anaerobic wastewater and fermentation exhaust can eliminate the inhibiting action of ammonia nitrogen on alcohol fermentation, enhances the feasibility of alcohol fermentation by recovering treated wastewater, implements the zero discharge of the wastewater and fermentation exhaust in the alcohol production process, solves the key technical bottle neck for restricting the clean production of alcohol fermentation, and obtains the high-value microalgae thallus product or biological grease.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for cultivating microalgae by using alcohol waste water and fermentation tail gas. Background technique [0002] Currently, traditional alcoholic fermentation processes produce CO 2 The tail gas from the fermentation process has not been rationally and effectively utilized, and all of it is released into the air as a greenhouse gas. The carbon recovery rate is low, which is not conducive to the mitigation of the greenhouse effect. There is a special unpleasant smell in the fermentation tail gas, which seriously affects the normal life of the surrounding residents. Therefore, the fermentation tail gas must be treated. Moreover, the traditional alcohol wastewater treatment usually adopts the "secondary anaerobic + aerobic" process. The anaerobic wastewater contains a large amount of organic acids, microbial cells, ammonia nitrogen and phosphorus, which are inhibi...

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Application Information

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IPC IPC(8): C02F9/14C02F3/32
Inventor 马晓建蒋清丽杨旭李洪亮王根灿
Owner 河南天冠生物燃料工程技术有限公司
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