Ultrasonic reinforcement culture method of microalgae

Abstract

The invention provides an ultrasonic reinforcement culture method of microalgae. The method includes selection and cleaning of a certain amount of algae, production of a microalgae solid medium, and conservation and spread culture of microalgae species in laboratory. The method is as below: first selecting a liquid medium appropriate for microalgae growth, and adding gel to obtain a solid medium; selecting sterile purified microalgae species, inoculating into the solid medium, sealing and culturing; and finally conducting initial expansion culture and relaying culture of microalgae species; and carrying out quality control on microalgae species, and screening out a high-quality microalgae species. Through ultrasonic reinforcement on high-quality microalgae species, cell wall partial rupture caused by ultrasonic improves the permeability of cell membrane, thus promoting the release of metabolites, promoting cell growth and metabolism, and increasing growth rate.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation and production, and in particular relates to a method for ultrasonically intensified microalgae cultivation that can be used to produce bioenergy, biomaterials, food, feed, health care products and medicines. Background technique [0002] An important reason why microalgae, as a valuable biological resource, has not been fully utilized is that there is no economical production system. At present, the methods used for microalgae cultivation include raceway culture method, photobioreactor culture method, etc. However, the raceway culture tank required for the raceway culture method can only cultivate a few species grown in selective media. Algae species, such as microalgae species such as spirulina and salina; while the closed photobioreactor system used in the photobioreactor culture method can cultivate most microalgae species, but the construction cost and operation cost are high. ...

AI Technical Summary

Problems solved by technology

Photobioreactors can provide a closed culture environment and controllable culture conditions. In theory, most microalgae species can be cultivated relatively well, which has been the focus of research and development in the industry for many years. However, there are still construction and The problem of high cost of use is only applicable to the production of high value-added microalgae products, and its application in the microalgae industry has certain limitations. A complete set of mature process routes has not yet been formed for the method of batch microalgae cultivation