Ultrasonic reinforcement culture method of microalgae

A technology of microalgae cultivation and ultrasonic enhancement, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high construction and use costs, and the method of batch microalgae cultivation does not form a process route, etc., to achieve Effects of promoting release, shortening the culture cycle, promoting growth and metabolism

Inactive Publication Date: 2015-09-09
邹宁
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Problems solved by technology

Photobioreactors can provide a closed culture environment and controllable culture conditions. In theory, most microalgae species can be cultivated relatively well, which has been the focus of research and development in the industry for many years. However, there are still construction and

Method used

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Embodiment 1

[0054] The present invention, a kind of method for supersonic strengthening microalgae culture, it specifically comprises the following steps:

[0055] Step 1, laboratory expansion of Haematococcus pluvialis

[0056] Firstly, the present invention preferably selects the BBM medium formula suitable for the growth of microalgae to configure the microalgae liquid medium, and adds 1% agarose gel to the liquid culture to make the solid medium;

[0057] Secondly, preserve and cultivate microalgae in the incubator, the culture temperature is 20°C, and the light intensity is about 30uE / m 2 / s;

[0058] Then, transfer the microalgae from the solid medium to a 200ml culture bottle containing 20mL of medium, and culture them in a rocking boat incubator for about 14-21 days at a culture temperature of 20°C and a light intensity of about 30uE / m 2 / s, shaker about 40rpm;

[0059] Finally, select the culture bottle whose color of the algae fluid turns green, observe the appearance of the ...

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Abstract

The invention provides an ultrasonic reinforcement culture method of microalgae. The method includes selection and cleaning of a certain amount of algae, production of a microalgae solid medium, and conservation and spread culture of microalgae species in laboratory. The method is as below: first selecting a liquid medium appropriate for microalgae growth, and adding gel to obtain a solid medium; selecting sterile purified microalgae species, inoculating into the solid medium, sealing and culturing; and finally conducting initial expansion culture and relaying culture of microalgae species; and carrying out quality control on microalgae species, and screening out a high-quality microalgae species. Through ultrasonic reinforcement on high-quality microalgae species, cell wall partial rupture caused by ultrasonic improves the permeability of cell membrane, thus promoting the release of metabolites, promoting cell growth and metabolism, and increasing growth rate.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation and production, and in particular relates to a method for ultrasonically intensified microalgae cultivation that can be used to produce bioenergy, biomaterials, food, feed, health care products and medicines. Background technique [0002] An important reason why microalgae, as a valuable biological resource, has not been fully utilized is that there is no economical production system. At present, the methods used for microalgae cultivation include raceway culture method, photobioreactor culture method, etc. However, the raceway culture tank required for the raceway culture method can only cultivate a few species grown in selective media. Algae species, such as microalgae species such as spirulina and salina; while the closed photobioreactor system used in the photobioreactor culture method can cultivate most microalgae species, but the construction cost and operation cost are high. ...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N1/12C12R1/89
Inventor 邹宁张承才郭素霞韩睿明张兆雷李悦明徐建春
Owner 邹宁
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