Method for purifying undiluted anaerobic fermentation tail liquid by growing microalgae in carbon dioxide environment

An anaerobic fermentation and carbon dioxide technology, applied in chemical instruments and methods, single-cell algae, multi-stage water treatment, etc., can solve the problems of algae growth inhibition, reactor volume increase, unfavorable photosynthesis, etc., and shorten growth lag period effect

Inactive Publication Date: 2016-03-16
ZHEJIANG UNIV
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Problems solved by technology

[0006] Due to the high turbidity of the high-concentration anaerobic fermentation tail liquid is not conducive to the progress of photosynthesis, and the high ammonia nitrogen content is also toxic to a certain extent, the direct cultivation of microalgae without dilution is likely to inhibit the growth of algae, so large Most studies use wastewater that has been diluted to varying degrees, and dilution of wastewater means that the amount of wastewater to be treated increases and the volume of the reactor required increases, which is not conducive to engineering applications
[0007] In order to solve the problem of tail liquid discharge after biogas fermentation and the cost of microalgae cultivation, a method that can not only purify the tail liquid but also obtain higher biomass quality of microalgae has broad application prospects

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  • Method for purifying undiluted anaerobic fermentation tail liquid by growing microalgae in carbon dioxide environment

Examples

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Effect test

Embodiment 1

[0029] Obtain the anaerobic fermentation tail liquid at the outlet discharge of the anaerobic fermentation tank with pig manure as the substrate. The COD content of the anaerobic fermentation tail liquid is 2000mg / L, centrifuge to remove suspended particles, and take the supernatant as raw material. The supernatant obtained after centrifugation was sterilized under saturated water vapor at 110° C. for 20 min. The pH of the tail liquid before sterilization was 7, and the pH of the tail liquid after sterilization was 9. Use 1mol / L sodium hydroxide and 1mol / L hydrochloric acid to adjust the pH to 6, take fresh natural Chlorella seed liquid, and inoculate it into the pH-adjusted tail solution according to the inoculum size of 0.1g / L. Under the condition of constant temperature of 25°C, light intensity of 5000lux, 12h of light and 12h of darkness every day, 30ml / min of high-concentration 15% carbon dioxide was passed into 300ml of anaerobic fermentation tail liquid and cultivated f...

Embodiment 2

[0031] Obtain the anaerobic fermentation tail liquid at the outlet discharge of the anaerobic fermentation tank with food waste as the substrate. The COD content of the anaerobic fermentation tail liquid is 3000mg / L, centrifuge to remove suspended particles, and take the supernatant as raw material. The supernatant obtained after centrifugation was sterilized under saturated water vapor at 115° C. for 25 min. The pH of the tail liquid before sterilization was 8, and the pH of the tail liquid after sterilization was 10. Use 1mol / L sodium hydroxide and 1mol / L hydrochloric acid to adjust the pH to 7, take the Nannochloropsis mutant liquid obtained by nuclear mutagenesis, and inoculate it into the tail liquid after adjusting the pH according to the inoculum size of 0.25g / L . At a constant temperature of 27.5°C, with a light intensity of 6000 lux, 12 hours of light and 12 hours of darkness every day, 45ml / min of high-concentration 15% carbon dioxide was introduced into the 300ml a...

Embodiment 3

[0033]The anaerobic fermentation tail liquid was obtained at the outlet discharge of the anaerobic fermentation tank with rice straw as the substrate. The COD content of the anaerobic fermentation tail liquid was 4000 mg / L, and the suspended particles were removed by centrifugation, and the supernatant was used as a raw material. The supernatant obtained after centrifugation was sterilized under saturated water vapor at 120° C. for 40 min. The pH of the tail liquid before sterilization was 9, and the pH of the tail liquid after sterilization was 11. Use 1mol / L sodium hydroxide and 1mol / L hydrochloric acid to adjust the pH to 8, take the spirulina seed liquid obtained through genetic modification, and inoculate it into the tail liquid after pH adjustment according to the inoculation amount of 0.5g / L. At a constant temperature of 30°C, with a light intensity of 7000 lux, 12 hours of light and 12 hours of darkness every day, 60ml / min of high-concentration 15% carbon dioxide was i...

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Abstract

The invention relates to purification of organic pollutants in wastewater, and provides a method for purifying undiluted anaerobic fermentation tail liquid by growing microalgae in a carbon dioxide environment. The method comprises the steps of centrifuging the anaerobic fermentation tail liquid, taking supernate as the tail liquid raw material, sterilizing the tail liquid raw material, regulating the pH value of the tail liquid raw material, and then conducting alga species inoculation; controlling temperature and illumination, and introducing carbon dioxide to the inoculated tail liquid raw material for 7-14 days of culture; obtaining microalgae biomass through centrifugal separation after microalgae culture is ended, so that purification of the anaerobic fermentation tail liquid is achieved. Optimal regulation is achieved by growing microalgae in the high-concentration carbon dioxide environment, anaerobic fermentation tail liquid purification and microalgae growth are combined, and organic pollutants in the undiluted pig manure anaerobic fermentation tail liquid can be effectively purified.

Description

technical field [0001] The invention relates to the field of purifying waste water organic pollutants, in particular to a method for purifying undiluted anaerobic fermentation tail liquid by growing microalgae under carbon dioxide. Background technique [0002] Biogas production by anaerobic fermentation is a widely used technology in rural areas, providing people with cheap and clean fuel. The by-products of fermentation, biogas slurry and residue, are usually discharged directly into water bodies or landfilled. The biogas fermentation process can remove a large amount of soluble organic matter in raw materials, but inorganic components such as ammonia nitrogen cannot be effectively removed (Parketal., 2010), and the tail liquid after fermentation still contains high COD, total nitrogen, total phosphorus and a large number of microorganisms , if discharged directly, it will cause eutrophication of water body and cause secondary pollution (Parketal., 2010). Large and mediu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C02F9/10
CPCC02F1/001C02F1/048C02F1/66C02F9/00C02F2301/08C12N1/12
Inventor 程军岑可法王智化刘建忠周俊虎杨卫娟张彦威黄镇宇周志军
Owner ZHEJIANG UNIV
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