Novel high pH induction and carbon dioxide emission reduction coupling microalgae harvesting method

A technology of carbon dioxide and a new method, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of large amount of addition, increased cost, waste of carbon sources, etc., to achieve high-efficiency carbon supplementation, reduce fertilizer costs, The effect of high-efficiency and low-cost harvesting

Active Publication Date: 2013-04-03
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF2 Cites 30 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method still has the problem of high cost, but because the pH range of the optimal growth of most unicellular microalgae currently cultivated is close to neutral (6.5-7.5), the cost of induction by adding alkali is relatively high, and the post-harvest A large amount of acid added to neutralize pH includes hydrochloric acid, sulfuric acid or carbon dioxide, which will increase new costs
At the same time, if this method uses hydrochloric acid and sulfuric acid to neutralize the high pH harvest supernatant, although the discharge of the medium can be reduced to a certain extent and the medium

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The MC-1 strain of Chlorococcum alkaliphilus comes from the South China Sea Institute of Oceanology, Chinese Academy of Sciences. It is used as the algal species, and the medium is modified based on ZSNT. 3 Modified to 5gL -1 , the others are the same, the initial pH value of the medium is 8.2. Use a 3L Erlenmeyer flask to add 2L medium, then inoculate the algae species, the culture OD after inoculation 700 0.2, the light intensity is 100μEm -2 the s -1 , the culture temperature is 25°C, and the photoperiod is 14h:10h (light: dark). Under light conditions, shake well every 4 hours and check the pH of the algae solution. If the pH reaches 9.5-10, inject CO 2 After adjusting the medium to pH 8.2-8.5, stop CO supplementation 2 , so repeatedly, the pH value of the control medium is between 8.2 and 9.5. After the algae liquid has been cultured for 7 days, the concentration of algae cells reaches the level to be harvested at 0.8g / L, and the CO supplementation is stopped...

Embodiment 2

[0018] The MC-1 strain of Chlorococcum alkaliphilus comes from the South China Sea Institute of Oceanology, Chinese Academy of Sciences. It is used as the algae species. The medium is modified based on ZSNT, and NaHCO3 is modified to 5gL -1 , the initial pH of the medium was 8.2. Add 9.6L of culture medium into 20L glass tank reactor, that is, insert 6.4L of Chlorococcum alkaliphilus MC-1 algae liquid (cultivated with the culture medium of this example) into 9.6L of culture medium, and inoculate after culture OD 700 0.3, outdoor natural light cultivation. Under light conditions, stir once every 4 hours, and check the pH of the algae liquid, if the pH reaches 9.5-10, pass CO 2 After adjusting the medium to pH 8.2-9, stop CO supplementation 2 , so repeatedly, the pH value of the control medium is between 8.2 and 9.5. After 3 days of algae culture, the concentration of algae cells reaches the level to be harvested 1g / L, stop supplementing CO 2 , no longer to control the pH o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a novel high pH induction and carbon dioxide emission reduction coupling microalgae harvesting method. Microalgae cells are self-settled and efficiently concentrated by using high pH induction so that high-efficiency and low-cost harvesting is realized. A CO2 emission reduction carbon replenishing technology is coupled, acidic gas CO2 is efficiently absorbed by using harvested high-alkali supernate to ensure that the pH of the high-alkali supernate is reduced to be a level (pH>=8.2) suitable for microalgae growth, high-efficiency carbon replenishing of a culture medium is realized, the supernate after havesting can be recycled, and the harvesting cost and fertilizer cost during large-scale cultivation of the microalgae are greatly lowered.

Description

Technical field: [0001] The invention relates to a method for harvesting microalgae cultures, in particular to a new method for harvesting microalgae induced by high pH and coupled with carbon dioxide emission reduction. Background technique: [0002] Microalgae are rich in nutrition, rich in protein, vitamins and polyunsaturated fatty acids, and have excellent application prospects in industrial fields such as health food, biological bait, medicine, and biofuel. However, the key problem currently hindering the development of the microalgae industry is the high cost of cultivation and production, and the cost of harvesting is one of the main factors limiting the cost of microalgae cultivation. In the culture of microalgae, the chemical flocculation method, that is, adding a small amount of macromolecular or small molecular compounds that can flocculate cells into the culture, concentrates the cells, and then centrifuges or filters the concentrated cells to achieve cell colle...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/12C12R1/89
Inventor 张峰向文洲吴华莲何慧萧邶叶永辉范洁伟
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap