Coding gene of chlorella endophyte and specific primer for amplifying coding gene

A chlorella, endophyte technology, applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc., can solve the problems of neglect of microbial groups, lack of external characteristics and corresponding effects, etc.

Active Publication Date: 2020-02-07
JIAXING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since Kloepper first proposed the concept of "plant endophytes", the research on plant endophytes at home and abroad has a history of more than 100 years, but because they do not show obvious external characteristics and corresponding functions , resulting in this microbial group being neglected by people for a long time

Method used

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  • Coding gene of chlorella endophyte and specific primer for amplifying coding gene
  • Coding gene of chlorella endophyte and specific primer for amplifying coding gene
  • Coding gene of chlorella endophyte and specific primer for amplifying coding gene

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Embodiment Construction

[0025] The technical solution of the present invention will be further specifically described below through specific examples. It should be understood that the implementation of the present invention is not limited to the following examples, and any modifications and / or changes made to the present invention will fall within the protection scope of the present invention.

[0026] In the present invention, unless otherwise specified, all parts and percentages are in weight units, and the equipment and raw materials used can be purchased from the market or commonly used in the field. The methods in the following examples, unless otherwise specified, are conventional methods in the art.

[0027] Example Isolation, purification and identification of Chlorella endophytes

[0028] 1 Experimental materials

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Abstract

The invention relates to a strain for culturing aegagropila linnaei, in particular to a coding gene of chlorella endophyte and a specific primer for amplifying the coding gene, and belongs to the technical field of biology. The nucleotide sequence of the gene for coding the chlorella endophyte is as shown in SEQ ID No.1. The chlorella endophyte is a strain S395-2 of microbacterium bacteria (Microbacterium saccharophilum), the strain is preserved in the China General Microbiological Culture Collection Center on 30 July 2018, and the number is CGMCC No.16182. The invention relates to an application of the chlorella endophyte in the respect of promoting growth of chlorella. The chlorella endophyte S395-2 can be used as novel microorganism resources for promoting growth of microalgae and improving the growth quality of the microalgae.

Description

technical field [0001] The present invention relates to a kind of strain used for culturing coccus, in particular to a coding gene of chlorella endophyte and specific primers for amplification thereof, belonging to the field of biotechnology. Background technique [0002] Plant endophytes are a new type of sustainable microbial resource with broad application prospects. Since Kloepper first proposed the concept of "plant endophytes", the research on plant endophytes at home and abroad has a history of more than 100 years, but because they do not show obvious external characteristics and corresponding functions , leading to the long-term neglect of this microbial group. It was not until the end of the 20th century that Stierle et al. isolated and screened an endophytic fungus capable of producing paclitaxel in the tissue of Taxus brevifolia, and this research was paid attention to and carried out in depth, and then became a research hotspot at home and abroad. Researchers h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C12N15/11
CPCC07K14/195C12N15/11
Inventor 孙诗清赵永军胡长伟曹卫星刘娟孙辰
Owner JIAXING UNIV
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