Production method for high-quality sporophore of cordyceps militaris(L.)Fr. allowing carotenoid and cordycepin contents to be simultaneously increased

A carotene and cordycepin technology, which is applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., can solve the problems of cumbersome operation, complicated medium formula, etc., and achieves high commercial value, good appearance, and increased cordycepin content. Effect

Inactive Publication Date: 2019-04-30
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent "a method for increasing the content of cordycepin in the fruiting body of Cordyceps militaris CN 104221717A" increases the content of cordycepin in the fruiting body of Cordyceps militaris to 0.35% through zinc ion stress and adjustment of light conditions; Method for medium cordycepin content CN 105248150 A "obtain cordycepin fruiting body with 0.5% cordycepin content through high temperature stress, this method is limited to increase cordycepin content; patent "a Cordycepin culture medium and production method for increasing cordycepin content CN 105505784 A" increases the content of cordycepin in the fruiting body of Cordyceps militaris by adding nutrients such as adenine, soybean powder, sucrose and silkworm chrysalis powder to the formula of liquid culture medium; these methods have complicated medium formula and cumbersome operations, such as CN 105505784 The medium raw materials of A include millet, barley, soybean powder, sucrose, silkworm chrysalis powder, milk powder, brown sugar, peptone, adenine, soybean powder, etc.
So far, there is no report on the production method of simultaneously increasing the content of carotenoids and cordycepin in the fruiting bodies of Cordyceps militaris

Method used

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  • Production method for high-quality sporophore of cordyceps militaris(L.)Fr. allowing carotenoid and cordycepin contents to be simultaneously increased

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1, the cultivation of the fruiting body of Cordyceps militaris with high carotenoid and cordycepin content

[0035] In this example, the strain of Cordyceps militaris used was "Minyuan No. 1": it was purchased from the Strain Production Center of Minyuan High-tech Institute, Sichuan Province.

[0036] One, the preparation of culture medium of Cordyceps militaris

[0037] Mix 285g of rice granules and 15g of silkworm chrysalis powder evenly, divide them into 10 cultivation bottles, 30g / bottle, add 45g of nutrient solution to each bottle, seal, sterilize at 121°C for 1 hour, and wait for cooling to room temperature for later use, to obtain Culture bottle containing Cordyceps militaris cultivation medium.

[0038] 2. Preparation of seed solution

[0039] Prepare the seed liquid of Cordyceps militaris bacterial strain according to routine method, specifically as follows:

[0040] 1. Strain activation

[0041] Cordyceps militaris (Cordyceps militaris) strain "...

Embodiment 2

[0078] Example 2, Cultivation of Cordyceps militaris Fruiting Bodies with High Carotenoid and Cordycepin Contents

[0079] In this example, the Cordyceps militaris strain used is the Cordyceps militaris strain "Minyuan No. 1": purchased from the Strain Production Center of Minyuan High-Tech Institute, Sichuan Province.

[0080] One, the preparation of culture medium of Cordyceps militaris

[0081] Mix 285g naked oats and 15g silkworm chrysalis powder evenly, divide them into 10 cultivation bottles, 30g / bottle, add 45g nutrient solution to each bottle, seal, sterilize at 121°C for 1 hour, wait for cooling to room temperature for later use, and obtain Culture bottle containing Cordyceps militaris cultivation medium.

[0082] 2. Preparation of seed solution

[0083] The seed liquid of the Cordyceps militaris strain was prepared according to the conventional method, and the specific operation was referred to Step 2 of Example 1 to obtain the seed liquid.

[0084] 3. Inoculation a...

Embodiment 3

[0097] Example 3, Cultivation of Fruiting Bodies of Cordyceps militaris with High Carotenoid and Cordycepin Content High Light

[0098] In this example, the strain of Cordyceps militaris used is "Megasporehead Cordyceps militaris 09-888": purchased from Yatai Institute of Microbiology, Jinzhou, Liaoning.

[0099] One, the preparation of culture medium of Cordyceps militaris

[0100] Mix 285g of wheat and 15g of silkworm chrysalis powder evenly, divide them into 10 cultivation bottles, 30g / bottle, add 45g of nutrient solution to each bottle, seal, sterilize at 121°C for 1 hour, and wait for cooling to room temperature for later use to obtain Culture bottle of Cordyceps militaris culture medium.

[0101] 2. Preparation of seed solution

[0102] The seed liquid of the Cordyceps militaris strain was prepared according to the conventional method, and the specific operation was referred to Step 2 of Example 1 to obtain the seed liquid.

[0103] 3. Inoculation and cultivation to o...

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Abstract

The invention discloses a production method for a high-quality sporophore of cordyceps militaris(L.)Fr. allowing carotenoid and cordycepin contents to be simultaneously increased. A cultivation methodprovided by the invention divides a cultivation process of the sporophore of the cordyceps militaris(L.)Fr. into four different stages including spawn runing, primordium differentiation, sporophore growth and accumulation of metabolites, and temperature, light and humidity control are separately performed on the different stages. In the stages of the sporophore growth and the accumulation of themetabolites, based on white light irradiation, Led blue light irradiation is supplemented by utilizing a blue light strip, and sporophore samples are dried at low temperature by employing hot air or freeze-dried. By utilization of a conventional culture medium and through control of culture conditions by stages, a product of the sporophore of the cordyceps militaris(L.)Fr. is produced and obtained, allows carotenoid and cordycepin contents to be simultaneously increased, is deep yellow or orange in color, is good in appearance, and is high in commodity value, and the production method is simple and easy to implement, and can achieve high-quality production of the sporophore of the cordyceps militaris(L.)Fr..

Description

technical field [0001] The invention relates to a method for cultivating fruit bodies of Cordyceps militaris, in particular to a method for producing high-quality fruit bodies of Cordyceps militaris by simultaneously increasing the contents of carotenoids and cordycepin. Background technique [0002] Cordyceps militaris (L.) Fr., also known as Cordyceps militaris, is a rare edible and medicinal fungus with high nutritional value and nourishing effect. Biological taxonomy belongs to Ascomycota, Ascomycetes, Hypocreales, Cordicipitaceae, Cordyceps (Fr.) Link]. "Compendium of Xinhua Materia Medica" stated that Cordyceps militaris "whole herb: sweet in taste, flat in nature. It is beneficial to the lungs and kidneys, nourishes the essence, stops bleeding and resolves phlegm." Modern pharmacological research has also confirmed that Cordyceps militaris is anti-tumor, anti-virus, and lowers blood pressure. and other functions. Because its nourishing effect and medicinal effect ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00
Inventor 董彩虹王芬张娇娇
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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