Production method for high-quality sporophore of cordyceps militaris(L.)Fr. allowing carotenoid and cordycepin contents to be simultaneously increased
A carotene and cordycepin technology, which is applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., can solve the problems of cumbersome operation, complicated medium formula, etc., and achieves high commercial value, good appearance, and increased cordycepin content. Effect
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Embodiment 1
[0034] Embodiment 1, the cultivation of the fruiting body of Cordyceps militaris with high carotenoid and cordycepin content
[0035] In this example, the strain of Cordyceps militaris used was "Minyuan No. 1": it was purchased from the Strain Production Center of Minyuan High-tech Institute, Sichuan Province.
[0036] One, the preparation of culture medium of Cordyceps militaris
[0037] Mix 285g of rice granules and 15g of silkworm chrysalis powder evenly, divide them into 10 cultivation bottles, 30g / bottle, add 45g of nutrient solution to each bottle, seal, sterilize at 121°C for 1 hour, and wait for cooling to room temperature for later use, to obtain Culture bottle containing Cordyceps militaris cultivation medium.
[0038] 2. Preparation of seed solution
[0039] Prepare the seed liquid of Cordyceps militaris bacterial strain according to routine method, specifically as follows:
[0040] 1. Strain activation
[0041] Cordyceps militaris (Cordyceps militaris) strain "...
Embodiment 2
[0078] Example 2, Cultivation of Cordyceps militaris Fruiting Bodies with High Carotenoid and Cordycepin Contents
[0079] In this example, the Cordyceps militaris strain used is the Cordyceps militaris strain "Minyuan No. 1": purchased from the Strain Production Center of Minyuan High-Tech Institute, Sichuan Province.
[0080] One, the preparation of culture medium of Cordyceps militaris
[0081] Mix 285g naked oats and 15g silkworm chrysalis powder evenly, divide them into 10 cultivation bottles, 30g / bottle, add 45g nutrient solution to each bottle, seal, sterilize at 121°C for 1 hour, wait for cooling to room temperature for later use, and obtain Culture bottle containing Cordyceps militaris cultivation medium.
[0082] 2. Preparation of seed solution
[0083] The seed liquid of the Cordyceps militaris strain was prepared according to the conventional method, and the specific operation was referred to Step 2 of Example 1 to obtain the seed liquid.
[0084] 3. Inoculation a...
Embodiment 3
[0097] Example 3, Cultivation of Fruiting Bodies of Cordyceps militaris with High Carotenoid and Cordycepin Content High Light
[0098] In this example, the strain of Cordyceps militaris used is "Megasporehead Cordyceps militaris 09-888": purchased from Yatai Institute of Microbiology, Jinzhou, Liaoning.
[0099] One, the preparation of culture medium of Cordyceps militaris
[0100] Mix 285g of wheat and 15g of silkworm chrysalis powder evenly, divide them into 10 cultivation bottles, 30g / bottle, add 45g of nutrient solution to each bottle, seal, sterilize at 121°C for 1 hour, and wait for cooling to room temperature for later use to obtain Culture bottle of Cordyceps militaris culture medium.
[0101] 2. Preparation of seed solution
[0102] The seed liquid of the Cordyceps militaris strain was prepared according to the conventional method, and the specific operation was referred to Step 2 of Example 1 to obtain the seed liquid.
[0103] 3. Inoculation and cultivation to o...
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