Production method for golden yellow nostoc sphaeroides
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A production method and golden yellow technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as maintaining color traits, and achieve the effect of enriching varieties and meeting market needs
Active Publication Date: 2018-02-02
武汉藻尚健生物科技有限公司
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During the artificial cultivation process, there is a certain chance that Gexianmi will show emerald green, red, blue and golden yellow colors, but when these special colors of Gexianmi are used as parents to continue to cultivate, the offspring Gexianmi will return to dark green or black, the color trait of which cannot be maintained by breeding methods known in the prior art
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Embodiment 1
[0040]The original seed of Gexianmi used in this example was collected from the paddy field of Zouma Town, Hefeng, Hubei, and the purified algae species were obtained after separation and purification in our laboratory. Select the dark green spherical kudzu rice (φ4mm) with good elasticity and strong toughness as the matrix, rinse with tap water for 20 minutes, fully rinse with sterile water for 10 minutes, irradiate with UVB ultraviolet lamp for 10 minutes, soak in 0.15% sodium hypochlorite solution for 2 minutes, and then rinse with sterile water for 10 minutes. Rinse 3 times and then make an inoculum.
[0041] Prepare the first medium according to the following recipe: NaNO 3 0.21g / L, Ca(H 2 PO 4 ) 2 0.12g / L, MgK 2 (SO 4 ) 2 0.24g / L, NaHCO 3 0.15g / L,FeCl 2 10mg / L, Na 2 EDTA 2 .4mg / L,H 3 BO 3 3mg / L, MnCl 2 4H 2 O1.50mg / L, ZnSO 4 ·7H 2 O 0.16mg / L, CuSO 4 ·5H 2 O 0.08mg / L, Na 2 MoO 4 2H 2 O 0.4mg / L, CoCl 2 ·6H 2 O 0.04mg / L. Distilled water was used...
Embodiment 2
[0046] The original seed of Gexianmi used in this example was collected from the paddy field of Zouma Town, Hefeng, Hubei, and the purified algae species were obtained after separation and purification in our laboratory. Select black spherical kudzu rice (φ5mm) with good elasticity and strong toughness as the matrix, rinse with tap water for 20 minutes, fully rinse with sterile water for 10 minutes, irradiate with UVB ultraviolet lamp for 10 minutes, soak in 75% alcohol solution for 3 minutes, and rinse with sterile water 3 times, and then make the inoculum.
[0047] Prepare the first medium according to the following recipe: NaNO 3 0.5g / L, Ca(H 2 PO 4 ) 2 0.24g / L, MgK 2 (SO 4 ) 2 0.30g / L, NaHCO 3 0.10g / L, FeCl 2 10mg / L, Na 2 EDTA2.0mg / L, H 3 BO 3 5.0mg / L, MnCl 2 4H 2 O3.0mg / L, ZnSO 4 ·7H 2 O 0.16mg / L, CuSO 4 ·5H 2 O 0.08mg / L, Na 2 MoO 4 2H 2 O 0.8mg / L, CoCl 2 ·6H 2 O 0.12mg / L. Distilled water was used for the preparation of the medium, and the medi...
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Abstract
The invention belongs to the field of algae culture, particularly relates to the culture of nostoc sphaeroides, in particular to a production method for golden yellow nostoc sphaeroides. The production method comprises the following steps: 1) seed manufacture: selecting a nostoc sphaeroides parent body, carrying out sterilization processing to obtain an inoculum, putting the inoculum into a sterilized first culture medium to be subjected to aerated culture; 2) amplification culture: after the diameter of the nostoc sphaeroides is 0.5-1.0mm, according to a ratio of a nostoc sphaeroides cultureobtained in 1) to a first induction culture medium of 1 to (5 to 50), putting the nostoc sphaeroides into a culture container with the first induction culture medium to be subjected to open culture; 3) induction: transferring the nostoc sphaeroides of which the diameter is 1.5-2.5mm to a second induction culture medium to be subjected to illumination culture for 30-60 days, and thus obtaining thegolden yellow nostoc sphaeroides of which the diameter is 4-6mm. Nutrition salt, light intensity and light quality are regulated and controlled, the ratio of carotenoid in nostoc sphaeroides cells isimproved, so that the nostoc sphaeroides is golden yellow, the category of a nostoc sphaeroides product is enriched, and market requirements are met.
Description
technical field [0001] The invention belongs to the field of algae cultivation, and in particular relates to the cultivation of kudzu rice, in particular to a production method of golden kudzu rice. Background technique [0002] Gexianmi, scientific name Nostoc sphaeroides, is a freshwater microalgae belonging to Cyanophyceae, Nostocaceae, and Nostoc genus. It belongs to the same genus as Nostoc, but its shape is different. Its gum group is spherical, with a diameter of up to several centimeters. It grows in mountainous rice fields, shallow ponds, and sandstones in lakes and streams. It is one of the earliest prokaryotic photosynthetic organisms on the earth. [0003] Gexianmi is a famous native product in Hubei, its protein content is as high as 56%, and the phycobiliprotein has strong anti-oxidation and anti-cancer effects. Gexianmi contains a large amount of biologically active cyanobacteria polysaccharides, which have the functions of inhibiting the growth of cancer ce...
Claims
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