Method for rejuvenating cordyceps militaris strains and increasing sporocarp yield by mating type

A technology of Cordyceps militaris and mating type, which is applied in the field of rejuvenating Cordyceps militaris strains and improving the yield of fruiting bodies by using the mating type, can solve the problems such as the rapid reduction of the yield of fruiting bodies, and achieves the effects of improving the yield of fruiting bodies and increasing the yield of fruiting bodies.

Active Publication Date: 2014-03-05
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention provides a method for rapidly increasing the yield of fruit bodies, and at the same time overcomes the problem that the yield of fruit bodies is rapidly reduced due to multiple passages of bacterial strains

Method used

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  • Method for rejuvenating cordyceps militaris strains and increasing sporocarp yield by mating type
  • Method for rejuvenating cordyceps militaris strains and increasing sporocarp yield by mating type
  • Method for rejuvenating cordyceps militaris strains and increasing sporocarp yield by mating type

Examples

Experimental program
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Embodiment Construction

[0020] 1. Obtaining of single spore strain of Cordyceps militaris

[0021] a. Inoculate the original strain of Cordyceps militaris and the strain of Cordyceps militaris that have been passed down many times on PPDA medium, culture at 25°C for 15 days, take a small amount of conidia in a triangular flask that has been added with sterile Tween 80 solution, and vortex vigorously Filter, count the concentration of the spore suspension with a hemocytometer, and serially dilute to 200 spores / mL of the spore suspension.

[0022] b. Place a sterile cellophane disc with a diameter of 5 mm on a 90 mm PPDA medium plate, and use a micropipette to take 5 μL of the spore suspension and drop it in the center of the 5 mm circular cellophane.

[0023] c. Cultivate in a 25°C incubator for 20 hours until the spores have just germinated and elongated. Use an inverted microscope to check the spore germination on the disk in turn, and transfer the disk with only one spore and the germ tube has germ...

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Abstract

The invention relates to a method for rejuvenating cordyceps militaris strains and increasing sporocarp yield by a mating type. The method includes performing single spore isolation on cordyceps militaris conidiospores to obtain two different mating-type single-spore strains, and inoculating the mating-type single-spore strains to tussah pupas or a rice medium according to a specific proportion so as to obtain high-yield sporocarps; inoculating the strains subcultured for multiple times to the tussah pupas or the rice medium according to specific proportional quantities of mat-alpha and mat-HMG, wherein mixed strains relatively subcultured for multiple times have high sporocarp yield.

Description

technical field [0001] The invention relates to a method for distinguishing the mating type of Cordyceps militaris by means of molecular biology method, rejuvenating the strain of Cordyceps militaris by using a new inoculation method and improving the yield of fruit bodies. Background technique [0002] The mating type of fungi is the genetic basis for controlling their mating affinity and sexual reproduction. As far as we know, the mating system of fungi includes two types: homothalmic mating and heterothallic mating. The hyphae that germinate from the same spore can produce sexual spores through self-association. This self-fertile reproductive mode is called synthalial union, and homothallic union also includes primary homothallic union and secondary heterothallic union. Dikaryotic hyphae must be produced by the conjugation of hyphae of different sexes to have fruitiness and can produce sexual spores. This self-sterile sexual reproduction method is called heterothalline u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 黄勃王玉龙陈安徽
Owner ANHUI AGRICULTURAL UNIVERSITY
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