Method for preparing prednisolone through bio-fermentation in one step

A technology of biological fermentation and prednisolone, which is applied in the field of preparation of prednisolone, can solve the problems such as no process route and adjustment.

Inactive Publication Date: 2017-06-27
TIANJIN JINYAO GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, researchers have made more efforts to innovate and improve the single-step process. However, due to traditional empirical thinking, the adjustment of the process route has not yet been carried out.

Method used

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  • Method for preparing prednisolone through bio-fermentation in one step
  • Method for preparing prednisolone through bio-fermentation in one step
  • Method for preparing prednisolone through bio-fermentation in one step

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Arthrobacter Simple ATCC 21032 is cultured in slant medium, primary culture and secondary culture in sequence, and the culture temperature is 29-32°C, and hydrocortisone acetate dissolved in an organic solvent is put into the fermentation medium In the 5L fermenter, the feed concentration is shown in the table below, and the reaction temperature is 31±1°C. The reaction time is shown in the table below. After the reaction is completed, the temperature is raised to 70°C to terminate the reaction, the fermentation broth is extracted with ethyl acetate, the organic phase is concentrated, and the conversion rate of prednisolone is measured.

[0051]

Embodiment 2

[0053] The specific preparation method culture medium and feeding concentration are the same as in Example 1, except that Tween 80 is added to the 5L fermentation tank of the fermentation medium before cortisone acetate is put into use.

[0054]

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Abstract

The invention relates to a method for directly generating prednisolone through bio-fermentation in one step by using cortisone acetate as a raw material and Arthrobacter Simple ATCC 21032.

Description

[0001] Technical field: [0002] The invention relates to a preparation method of prednisolone, which is to prepare prednisolone from hydrocortisone acetate in one step by carrying out 1, 2-position dehydrogenation and 21 acetate hydrolysis reactions through biological fermentation. [0003] Background technique: [0004] Prednisolone, a common corticosteroid, can be used not only as a corticosteroid treatment drug, but also as a raw material for other corticosteroid drugs. It has wide application value and is very important. Research on the preparation of this product continues at home and abroad. [0005] [0006] The commonly used starting material for domestic production of prednisolone is mold oxide derived from diene, which is first oxidized at C11, then dehydrogenated at C1 and 2, and then modified at the side chain to obtain esterification at 21. The difficulty of this process lies in the introduction of C11-position carbonyl and C1, 2-position double bonds. Since t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/02C12P33/00C12R1/06
CPCC12P33/005C12P33/02
Inventor 王福军张杰王延年
Owner TIANJIN JINYAO GRP
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