Quick extraction and detection method of diethyl yellow in dried bean curd

A diethyl yellow and detection method technology, applied in the preparation of test samples, Raman scattering, material excitation analysis, etc., can solve the problem of low sensitivity, achieve accurate qualitative, accurate detection results, and suppress oil interference Effect

Inactive Publication Date: 2015-06-10
河北省食品检验研究院 +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Surface-enhanced Raman spectroscopy (SERS) uses trace molecules to adsorb on Cu, Ag, Au and other metal sols and electrode surfaces, and its Raman spectrum signal can be enhanced by 104 to 106 times, which overcomes the disadvantage of low sensitivity of conventional Raman spectroscopy

Method used

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  • Quick extraction and detection method of diethyl yellow in dried bean curd

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Effect test

Embodiment 1

[0035] A kind of rapid extraction and detection method of diethyl yellow in dried tofu, comprises the following steps:

[0036](1) Determine the characteristic Raman peak of diethyl yellow: According to the characteristic chemical structure of diethyl yellow, determine the 990cm -1 ±3cm -1 It is the characteristic Raman peak of diethyl yellow.

[0037] (2) Draw a diethyl yellow standard curve: Accurately weigh 0.1g (accurate to 0.001g) of diethyl yellow standard substance, dissolve it in acetonitrile, and set the volume to 100 mL. The concentration of this solution is 1000mg / L, -4 ℃ and protected from light. When used, it is gradually diluted into 1mg / L, 2mg / L, 4mg / L, 8mg / L, 10mg / L standard series of working solutions, and is detected by a laser Raman spectrometer, and the Raman spectrum of the diethyl yellow standard is analyzed. Baseline adjustment and normalization processing to obtain the standard curve.

[0038] (3) ASE extraction of diethyl yellow in the sample to be...

Embodiment 2

[0057] A kind of rapid extraction and detection method of diethyl yellow in dried tofu, comprises the following steps:

[0058] (1) Determine the characteristic Raman peak of diethyl yellow: According to the characteristic chemical structure of diethyl yellow, determine the 990cm -1 ±3cm -1 It is the characteristic Raman peak of diethyl yellow.

[0059] (2) Draw a diethyl yellow standard curve: Accurately weigh 0.1g (accurate to 0.001g) of diethyl yellow standard substance, dissolve it in acetonitrile, and set the volume to 100 mL. The concentration of this solution is 1000mg / L, -4 ℃ and protected from light. When used, it is gradually diluted into 1mg / L, 2mg / L, 4mg / L, 8mg / L, 10mg / L standard series of working solutions, and is detected by a laser Raman spectrometer, and the Raman spectrum of the diethyl yellow standard is analyzed. Baseline adjustment and normalization processing to obtain the standard curve.

[0060] (3) ASE extraction of diethyl yellow in the sample to b...

Embodiment 3

[0079] A kind of rapid extraction and detection method of diethyl yellow in dried tofu, comprises the following steps:

[0080] (1) Determine the characteristic Raman peak of diethyl yellow: According to the characteristic chemical structure of diethyl yellow, determine the 990cm -1 ±3cm -1 It is the characteristic Raman peak of diethyl yellow.

[0081] (2) Draw a diethyl yellow standard curve: Accurately weigh 0.1g (accurate to 0.001g) of diethyl yellow standard substance, dissolve it in acetonitrile, and set the volume to 100 mL. The concentration of this solution is 1000mg / L, -4 ℃ and protected from light. When used, it is gradually diluted into 1mg / L, 2mg / L, 4mg / L, 8mg / L, 10mg / L standard series of working solutions, and is detected by a laser Raman spectrometer, and the Raman spectrum of the diethyl yellow standard is analyzed. Baseline adjustment and normalization processing to obtain the standard curve.

[0082] (3) ASE extraction of diethyl yellow in the sample to b...

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Abstract

The invention discloses a quick extraction and detection method of diethyl yellow in dried bean curd, and relates to the technical field of food detection. The method comprises the following steps: determining characteristic raman peak 990+/-3 cm<-1> of the diethyl yellow; drawing a diethyl yellow standard curve; weighing the bean curd, mixing with diatomite and then adding in an extraction pond held with a cellulose membrane; filling the spare volume with Ottawa sand; extracting the diethyl yellow by using ASE (Accelerated Solvent Extraction) by using acetonitrile water solution with volume concentration of 75-85 percent as an extracting agent; evaporating, concentrating and extracting the solution until to dry, adding acetonitrile to dissolve, centrifuging, weighing supernatant, adding sodium chloride solution, oscillating and centrifuging, wherein the supernatant is to-be-tested sample solution; adding gold nanosol and an inorganic salt flocculant into the to-be-tested sample solution, uniformly shaking, and detecting the sample raman spectrum chart by using a raman spectrometer; performing qualitative and quantitative determination. The method has the characteristics of high extraction speed, full extraction, easiness in operation, accurate detection, quickness, no damage and low cost, and foods with problems can be effectively distinguished.

Description

technical field [0001] The invention relates to the technical field of food detection. Background technique [0002] Dried tofu is a kind of food made of soybeans mixed with other raw materials. The production process generally includes: grinding, slag removal, cooking, paste preparation, powder test, paste powder, mixing and stewing, massaging, briquetting, Cooked, some are also colored with gardenia yellow after cooking. Some criminals use low-quality soybeans to produce dried tofu. The taste and color of the dried tofu produced in this way are far from normal dried tofu. At this time, in order to increase its appearance and taste, manufacturers use illegal additives and cheap non-edible Pigments (such as diethyl yellow), etc. According to recent news reports, dried tofu products produced by many bean product processing manufacturers in Taiwan were found to contain diethyl yellow. Diethyl yellow is a yellow powder, mainly used for the coloring of paraffin, polyvinyl chl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/28
Inventor 李挥蔡立鹏张岩张丽冰闫正任孟伟李霏盖丽娜刘连太张永辉
Owner 河北省食品检验研究院
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