Quick extraction and detection method of diethyl yellow in dried bean curd

Abstract

The invention discloses a quick extraction and detection method of diethyl yellow in dried bean curd, and relates to the technical field of food detection. The method comprises the following steps: determining characteristic raman peak 990+ / -3 cm<-1> of the diethyl yellow; drawing a diethyl yellow standard curve; weighing the bean curd, mixing with diatomite and then adding in an extraction pond held with a cellulose membrane; filling the spare volume with Ottawa sand; extracting the diethyl yellow by using ASE (Accelerated Solvent Extraction) by using acetonitrile water solution with volume concentration of 75-85 percent as an extracting agent; evaporating, concentrating and extracting the solution until to dry, adding acetonitrile to dissolve, centrifuging, weighing supernatant, adding sodium chloride solution, oscillating and centrifuging, wherein the supernatant is to-be-tested sample solution; adding gold nanosol and an inorganic salt flocculant into the to-be-tested sample solution, uniformly shaking, and detecting the sample raman spectrum chart by using a raman spectrometer; performing qualitative and quantitative determination. The method has the characteristics of high extraction speed, full extraction, easiness in operation, accurate detection, quickness, no damage and low cost, and foods with problems can be effectively distinguished.

Description

technical field

[0001] The invention relates to the technical field of food detection. Background technique

[0002] Dried tofu is a kind of food made of soybeans mixed with other raw materials. The production process generally includes: grinding, slag removal, cooking, paste preparation, powder test, paste powder, mixing and stewing, massaging, briquetting, Cooked, some are also colored with gardenia yellow after cooking. Some criminals use low-quality soybeans to produce dried tofu. The taste and color of the dried tofu produced in this way are far from normal dried tofu. At this time, in order to increase its appearance and taste, manufacturers use illegal additives and cheap non-edible Pigments (such as diethyl yellow), etc. According to recent news reports, dried tofu products produced by many bean product processing manufacturers in Taiwan were found to contain diethyl yellow. Diethyl yellow is a yellow powder, mainly used for the coloring of paraffin, polyvinyl chl...

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