Constructing method and application of zebra fish bacterial infection model

A technology of bacterial infection and construction method, applied in application, fish farming, climate change adaptation, etc., can solve problems such as differences in physiological structure and physiological functions, poor prediction reliability and comparability, and large differences in biological levels, etc., to achieve repeatability Stable and reliable performance, simple experimental process operation, high success rate effect

Active Publication Date: 2013-02-06
南京新环检测科技有限公司 +1
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, lower animals such as fruit flies and nematodes are too different from humans in terms of biological levels, their gene similarity is low, and their physiological structures and functions are quite different. Therefore, the prediction reliability and comparability of their drug test results are poor.

Method used

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  • Constructing method and application of zebra fish bacterial infection model
  • Constructing method and application of zebra fish bacterial infection model
  • Constructing method and application of zebra fish bacterial infection model

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Embodiment Construction

[0050] The object of the present invention is to provide a construction method and application of a zebrafish bacterial infection model. The method provided by the invention has the advantages of simplicity, rapidity, economy, high efficiency, high throughput and the like.

[0051] One, the present invention provides a kind of method that establishes zebrafish bacterial infection model, design scheme is:

[0052] 1. Culture of Bacteria

[0053] Transfer the red fluorescent plasmid RFP(Kan+) with kanamycin resistance into chloramphenicol-resistant Escherichia coli BL21(DE3)Plyss(Chl+), after successful transformation, apply LB solid plates (Kan+, Chl+) for screening Escherichia coli (hereinafter referred to as BL21RFP) capable of producing red fluorescence and having kanamycin resistance and chloramphenicol resistance, after growing a single colony, store the plate at 4°C. One day before each zebrafish bacterial injection, a single colony of BL21RFP was picked and placed in L...

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Abstract

The invention relates to a constructing method of a zebra fish bacterial infection model and application of the animal model to bacterial infection research and antibiotic screening. The constructing method of the zebra fish bacterial infection model mainly includes the steps of zebra fish selection, bacterium injection, image analysis and / or microwell plate analysis and statistical analysis. The constructing method has the advantages of simplicity, convenience, rapidity, economy, high efficiency, high throughput and the like, and the model can be used for bacterial infection research and antibiotic screening. The constructing method and application of the zebra fish bacterial infection model are of great significance to acceleration of research and development on antibiotics and improvement on treatment of patients suffering from bacterial infection.

Description

technical field [0001] The invention belongs to the field of biological model construction, and in particular relates to a construction method and application of a zebrafish bacterial infection model. Background technique [0002] Bacteria resistance is a thorny problem in the control and treatment of bacteria, and bacteria resistant to antibiotics is becoming a growing threat and becoming more and more common. The overuse of antibiotics has led to multi-drug resistance of bacteria, which makes humans face the danger of "super bacteria infection" [1]. Escherichia coli is the gram-negative bacteria most likely to develop drug resistance. The enterohaemorrhagic Escherichia coli (EHEC) epidemic that broke out in Germany in May 2011 was caused by a type of enterohaemorrhagic Escherichia coli (EHEC) called "husec41". (EHEC) variants that are resistant to many antibiotics. The abuse of antibiotics in animal husbandry has also led to a certain amount of antibiotic resistance of E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李春启张勇劳乔聪朱凤郭胜亚
Owner 南京新环检测科技有限公司
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