African swine fever virus fluorescent PCR (Polymerase Chain Reaction) detection reagent and detection method of thereof

Abstract

The invention relates to the technical field of African swine fever virus detection and discloses an African swine fever virus fluorescent PCR (Polymerase Chain Reaction) detection reagent and a detection method thereof. The detection reagent comprises a pair of PCR specific primers, a specific probe, an interior label probe and a PCR mixed liquid. The detection reagent adopts an African swine fever virus VP72 gene which is highly conservative as a target gent. The African swine fever virus fluorescent PCR detection reagent and the detection method thereof have the advantages that the Africanswine fever virus fluorescent PCR detection reagent is simple to use, no multiple special instrument are needed, the detection time is short, the detection efficiency is high, multiple operators are not needed, the labor is saved, and the like.

Description

technical field [0001] The invention relates to the technical field of African swine fever detection, in particular to a fluorescent PCR detection reagent for African swine fever and a detection method thereof. Background technique [0002] African swine fever is a severe, hemorrhagic infectious disease of pigs caused by African swine fever virus, with a fatality rate close to 100%. It was first discovered by Montgomery in Kenya, Africa in 1921. Because it is very harmful to the pig industry, and there is no effective Therefore, it is listed by OIE as the first legally reported infectious disease of pigs, and is highly concerned by countries all over the world. Since African swine fever virus has a complex immune evasion mechanism, lacks typical neutralizing antibodies, and has no effective vaccine for epidemic prevention, it is particularly important to establish a rapid and accurate detection method, although my country has not found this yet. However, with the increasing ...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a fluorescent PCR detection reagent for African swine fever and a detection method thereof, which has the advantages of simple use of the fluorescent PCR detection reagent for African swine fever, no need for various professional instruments, short detection time, and high detection efficiency. Higher, does not require multi-person cooperation, saves labor and other advantages, solves the problems of inconvenient African swine fever virus detection, low detection accuracy, low detection efficiency, and waste of labor