Quick detection method of residual sulfur dioxide in extract of natural plant

A technology of sulfur dioxide and natural plants, which is used in the detection of residual sulfur dioxide in natural plant extracts in health food, and the detection field of Raman spectrum characteristic peak signal intensity, which can solve problems such as low sensitivity, achieve high sensitivity, reduce usage, The effect of reducing hazards for operators

Inactive Publication Date: 2019-05-17
河北省食品检验研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surface-enhanced Raman spectroscopy (SERS) uses trace molecules to adsorb on Cu, Ag, Au and other metal sols and electrode surfaces, and its Raman spectrum signal can be enhanced by 10 4 ~10 6 , overcoming the shortcomings of low sensitivity of conventional Raman spectroscopy

Method used

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  • Quick detection method of residual sulfur dioxide in extract of natural plant
  • Quick detection method of residual sulfur dioxide in extract of natural plant
  • Quick detection method of residual sulfur dioxide in extract of natural plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Qualitative and quantitative analysis of residual sulfur dioxide in the extract of Atractylodes macrocephala in embodiment 1

[0093] Sample: taken from commercial Atractylodes macrocephala extract.

[0094] a. Weigh 1 gram of sample, grind it into powder in a 10mL centrifuge tube, add 4mL of pure water for oscillation, and place it in an ultrasonic extractor for 10 minutes of sonication.

[0095] b. Take 1.5mL extract in a 2mL centrifuge tube and centrifuge in a centrifuge for 2 minutes (10000 rpm).

[0096] c. Take 1mL supernatant to a 2mL centrifuge tube, add 200μL OTR-FB-PR-A and 200μL OTR-FB-PR-B in sequence, vortex for 1 minute, and centrifuge in a centrifuge for 2 minutes (10000 rpm ).

[0097] d. Take 500 μL of supernatant in a 2 mL centrifuge tube, add 50 μL of OTR-FB-SF, mix, and the resulting solution is the solution to be tested.

[0098] Qualitative analysis:

[0099] Take 0.1mL of the solution to be tested, add 0.3mL of gold nano-sol, 0.1mL of 1% sodiu...

Embodiment 2

[0104] Qualitative and quantitative analysis of residual sulfur dioxide in the extract of Angelica sinensis in embodiment 2

[0105] Sample: taken from a commercially available Angelica extract.

[0106] a. Weigh 5 grams of sample, grind it into powder, put it in a 50mL centrifuge tube, add 25mL of pure water to shake, and place it in an ultrasonic extractor for 10 minutes of sonication.

[0107] b. Take 1.5mL extract in a 2mL centrifuge tube and centrifuge in a centrifuge for 2 minutes (10000 rpm).

[0108] c. Take 1mL supernatant to a 2mL centrifuge tube, add 200μL OTR-FB-PR-A and 200μL OTR-FB-PR-B in sequence, vortex for 1 minute, and centrifuge in a centrifuge for 2 minutes (10000 rpm ).

[0109] d. Take 500 μL of supernatant in a 2 mL centrifuge tube, add 50 μL of OTR-FB-SF, mix, and the resulting solution is the solution to be tested.

[0110] Qualitative analysis:

[0111] Take 0.1mL of the solution to be tested, add 0.4mL of gold nano-sol, 0.5mL of 1% sodium chloride...

Embodiment 3

[0113] Qualitative and quantitative analysis of residual sulfur dioxide in the extract of Angelica sinensis in embodiment 3

[0114] Sample: taken from a commercially available Angelica extract.

[0115] a. Weigh 1 gram of sample, grind it into powder in a 10mL centrifuge tube, add 6g of pure water to shake, and place it in an ultrasonic extractor for 10 minutes of sonication.

[0116] b. Take 1.5mL extract in a 2mL centrifuge tube and centrifuge in a centrifuge for 2 minutes (10000 rpm).

[0117] c. Take 1mL supernatant to a 2mL centrifuge tube, add 200μL OTR-FB-PR-A and 200μL OTR-FB-PR-B in sequence, vortex for 1 minute, and centrifuge in a centrifuge for 2 minutes (10000 rpm ).

[0118] d. Take 500 μL of supernatant in a 2 mL centrifuge tube, add 50 μL of OTR-FB-SF, mix, and the resulting solution is the solution to be tested.

[0119] Qualitative analysis:

[0120] Take 0.1mL of the solution to be tested, add 0.3mL of silver nano-sol and 0.1mL of 1% potassium chloride ...

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Abstract

The invention relates to a quick detection technology of residual sulfur dioxide in extract of natural plant, and specially relates to a detection method adopting Raman spectrum characteristic peak signal intensity, and a residual sulfur dioxide detection method suitable for the extract of the natural plant in the healthcare food. By adopting the Raman spectrum characteristic peak signal intensity, the 618cm-1(+/-3cm-1) is used as the characteristic Raman peak, the qualitative analysis is accurate, the whole detection process is accomplished in 15 min, and the quick qualitative and quantification, the convenience and the low cost are realized.

Description

technical field [0001] The invention relates to a rapid detection technology for residual sulfur dioxide in natural plant extracts, in particular to a detection method using Raman spectrum characteristic peak signal intensity, which is suitable for the detection method of residual sulfur dioxide in natural plant extracts in health food. Background technique [0002] Sulfur dioxide has bleaching properties. Sulfur dioxide is commonly used in industry to bleach paper pulp, wool, silk, straw hats, etc. The bleaching effect of sulfur dioxide is due to the fact that it (sulfurous acid) can form unstable colorless substances with certain colored substances. This colorless substance is easy to decompose and restore the colored substance to its original color, so the straw hat braids bleached with sulfur dioxide turn yellow again over time. The bleaching effect of sulfur dioxide and certain sulfur-containing compounds is also illegally used by some unscrupulous manufacturers to pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/40
Inventor 张兰天王红李挥史国华刘东张斌马宁常化仿
Owner 河北省食品检验研究院
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