Detection kit for antiepileptic drugs in serum and application thereof

Abstract

The invention discloses a detection kit for antiepileptic drugs in serum and application thereof, and belongs to the technical field of drug analysis. The kit disclosed by the invention adopts an LC-MS / MS method; ten common antiepileptic drugs can be detected at one time; the kit is simple in pretreatment process, low in cost, high in sensitivity and high in specificity; separation and detection of the antiepileptic drugs are completed within 6 min, the accuracy and precision basically meet the requirements, the kit can be used for quantitative analysis of the clinical antiepileptic drugs, anda reliable detection method is provided for monitoring the treatment concentration of the clinical antiepileptic drugs.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a detection kit for antiepileptic drugs in serum and an application thereof. Background technique [0002] Epilepsy is a chronic disease characterized by sudden abnormal discharge of brain neurons, characterized by recurrent, paroxysmal, and stereotyped central nervous system dysfunction, leading to transient brain dysfunction. The etiology of epilepsy is complex and diverse, including genetic factors, brain diseases, systemic or systemic diseases, etc. Epilepsy is the second most persistent disease among nervous system diseases after cerebrovascular diseases. There are about 9 million epilepsy patients in China, of which 5 to 6 million are active epilepsy patients. Epilepsy has become the second most common disease in neurology after headache in China. When epileptic patients have seizures, motor and sensory functions, autonomic nervous states, and levels of ...

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Problems solved by technology

Since epilepsy requires long-term maintenance treatment, but the effective blood concentration range of antiepileptic drugs is narrow, and there are large individual differences in the metabolic process in the body, drug toxicity events are more likely to occur, and the pharmacokinetic characteristics are closely related to the antiepileptic effect and the occurrence of adverse drug reactions. Therefore, the monitoring of the drug concentration in its blood is an important reference for realizing individualized drug administration, reducing adverse reactions and avoiding the vicious cycle of drug use, which is of great significance in clinical drug use

[0004] At present, the methods for measuring the plasma concentration of antiepileptic drugs at home and abroad mainly use high-performance liquid chromatography and immunoassay. The article "Simple and Accurate Quantitative Analysis of Ten Antiepileptic Drugs in Human Plasma by Liquid Chromatography / Tandem Mass Spectrometry" reported that ten antiepileptic drugs were detected simultaneously. In the antiepileptic drug method, only two isotope internal standards are used for ten substances, the matrix interference will be relatively large, and the sample collection time will be relatively long, which takes more than 10 minutes per injection; the article "Development, validation and clinical application of an online-SPE-LC- HRMS / MS for simultaneous quantification of phenobarbital, phenytoin, carbamazepine, and its active metabolite carbamazepine10,11-epoxide" introduced online solid phase extraction processing samples, high configuration requirements, long sample collection time, etc.

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