Fermented cordyceps taishanensis powder, preparation sterols HPLC fingerprint spectrum and building method thereof

A technology of fingerprinting and bacterial powder sterols, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as the difficulty in achieving effective control of the quality of fermented Cordyceps powder and its preparations, and the lack of a quality evaluation system

Active Publication Date: 2018-09-14
JIANGXI GUOYAO PHARMA LLC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quality control of fermented Cordyceps powder and its preparations is mainly based on nucleoside chemical components, while sterols are rarely used as quality control targets, and there are few studies on the f

Method used

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  • Fermented cordyceps taishanensis powder, preparation sterols HPLC fingerprint spectrum and building method thereof
  • Fermented cordyceps taishanensis powder, preparation sterols HPLC fingerprint spectrum and building method thereof
  • Fermented cordyceps taishanensis powder, preparation sterols HPLC fingerprint spectrum and building method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Example 1. Construction method of sterol HPLC fingerprint of Jinshuibao tablet and its fingerprint

[0094] (1) Preparation of reference solution

[0095] Accurately weigh appropriate amounts of ergosterol, stigmasterol, β-sitosterol, and carotinoside, put them in the same 10 mL volumetric flask, add methanol to ultrasonically dissolve, and dilute to the mark to obtain the reference solution.

[0096] (2) Preparation of test solution

[0097] Weigh 1.5g each of 10 batches of Jinshuibao tablets (batch numbers are 160401, 160501, 160502, 160601, 160701, 160702, 160703, 161202, 161203, 161204), grind them into fine powder, add 10ml of methanol and extract for 60 minutes by ultrasonic, take it out and cool to room temperature After centrifugation at 8000r / min for 10min, take the supernatant through a 0.22μm microporous membrane, dilute the filtrate to 10ml with methanol, take an appropriate amount of the extract after constant volume, and add potassium hydroxide with a concentratio...

Embodiment 2

[0108] Construction method of HPLC fingerprint of sterols in Jinshuibao capsule and its fingerprint

[0109] (1) Preparation of reference solution

[0110] Accurately weigh appropriate amounts of ergosterol, stigmasterol, β-sitosterol, and carotinoside, put them in the same 10 mL volumetric flask, add methanol to ultrasonically dissolve, and dilute to the mark to obtain the reference solution.

[0111] (2) Preparation of test solution

[0112] Weigh 1.5g of Jinshuibao capsules for 10 times (batch numbers are 16010005, 16070345, 16080393, 16110701, 16090394, 16030133, 16050229, 16040185, 16020075, 16050347), add 10ml of methanol, and extract for 60min. After cooling to room temperature, centrifuge at 8000r / min. 10min, take the supernatant and pass it through a 0.22μm microporous filter membrane, dilute the filtrate to 10ml with methanol, take an appropriate amount of the extract after the constant volume, add a potassium hydroxide-methanol solution with a concentration of 0.4mol / L and ...

Embodiment 3

[0123] The method for constructing the fingerprint of the fermented cordyceps powder sterol of the present invention is optimized and finally determined after a large number of experiments. The preferable experiments are as follows:

[0124] 1. Optimization of chromatographic conditions

[0125] (1) Selection of elution system

[0126] Except for the elution system conditions, the other basic steps are the same as those of the test product for chromatographic detection. In the experiment, the methanol-water, acetonitrile-water, methanol-0.1% phosphoric acid water, acetonitrile-0.1% phosphoric acid water elution system were selected for research. The results show that the response of each component of the methanol-0.1% phosphoric acid water elution system is relatively small, and the resolution is poor, while the sterol components of the acetonitrile-water and acetonitrile-0.1% phosphoric acid water elution system do not peak in the corresponding time, so choose Methanol-water is us...

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Abstract

The invention belongs to the field of drug analysis, relates a quality control method of fermented cordyceps taishanensis powder, and particularly relates to fermented cordyceps taishanensis powder, preparation sterols HPLC fingerprint spectrum and a building method thereof. The building method includes the steps of firstly, preparing a control solution; secondly, preparing a test solution; thirdly, using HPLC to determine the fingerprint spectrum, and performing similarity evaluation, to be more specific, separately precisely sucking the control solution and the test solution, performing HPLCseparation detection with the flow phase being a methanol-water elution system to obtain the sterol fingerprint spectrum of the fermented cordyceps taishanensis powder, and importing the fingerprintspectrum into similarity software to perform similarity evaluation.

Description

Technical field [0001] The invention belongs to the technical field of drug analysis, and relates to a quality control method of fermented cordyceps powder, in particular to a method for constructing a sterol HPLC fingerprint map of fermented cordyceps powder and its preparation and its fingerprint. Background technique [0002] Cordyceps sinensis is a dry complex of the larvae of Cordyceps sinensis (Cordyceps sinensis) parasitizing on the larvae of Hematidae insects. It is one of the precious Chinese medicinal materials in my country. Due to the over-exploitation of Cordyceps resources, coupled with the specificity of its host and environmental limitations, Cordyceps resources are gradually depleted. Fermented Cordyceps fungus powder is a fungus powder obtained by separating medicinal strains from Cordyceps sinensis through deep fermentation. Research has found that fermented cordyceps fungus powder has similar chemical components and pharmacological effects to Cordyceps sinensi...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 陈丽华朱卫丰管咏梅杨明金晨赵加茜
Owner JIANGXI GUOYAO PHARMA LLC
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