Separation method of effective components of sharpleaf galangal fruit aqueous extract

Abstract

The invention discloses a separation method of effective components of a sharpleaf galangal fruit aqueous extract, belonging to the field of pharmaceutical analysis. The separation method comprises the following steps: (A) fetching dried sharpleaf galangal fruit, grinding, carrying out reflux extraction with water, combining filtrate, carrying out reduced pressure distillation to obtain extractum, dissolving the extractum into distilled water, respectively extracting by virtue of petroleum ether, ethyl acetate and normal butanol, and recycling solvents, so as to obtain ethyl acetate extract dry powder; (B) dissolving the dry powder obtain in the step (A), filtering, and processing by virtue of a sephadex LH20 gel column, so as to obtain six components from F1 to F6; and (C) carrying out HPLC separation and purification on the components from F2 to F6, so as to obtain compounds including protocatechualdehyde, protocatechuic acid, p-coumaric acid, (-)-epicatechin and (+)-catechinic acid. According to the separation method, the effective active components are separated and extracted from the sharpleaf galangal fruit, the structures of the effective components are further defined, the basis is provided for later medication, and required components can be extracted according to different requirements.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to the separation and purification of active ingredients of traditional Chinese medicines. Background technique [0002] Yizhiren is the dry and mature fruit of A.oxyphylla Miq., a plant of the genus Alpinia Roxb. in the Zingiberaceae family. It is one of the four major southern medicines in China and is mainly produced in Hainan and Guangdong. For commonly used Chinese herbal medicines. Yizhiren has the functions of warming the kidney, consolidating essence and shrinking urination, warming the spleen, stopping diarrhea, and absorbing saliva. It is mostly used clinically for enuresis due to kidney deficiency, frequent urination, white turbid spermatorrhea, diarrhea due to spleen cold, cold pain in the abdomen, and excessive saliva. Modern pharmacological studies have shown that the water extract of Yizhiren has neuroprotective and antioxidant effects, and Yizhiren also has the function...

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