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Method for determining medicine active component in serum

A technology of drug activity and serum, which is applied in the field of determination of active ingredients of drugs in serum and determination of active ingredients of compound Chinese medicine in serum

Active Publication Date: 2012-06-06
HEHUANG PHARMA SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the existence of numerous chemical components, it is quite difficult to study the material basis of the compound Chinese medicine and the mechanism of action of the compound Chinese medicine.

Method used

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  • Method for determining medicine active component in serum
  • Method for determining medicine active component in serum
  • Method for determining medicine active component in serum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Instruments and reagents involved in the present invention are:

[0049] Instrument: Agilent1100 chromatographic system and Chemstation chromatographic workstation, Agilent LC / MSD Trap XCT electrospray mass spectrometer detector; chromatographic column: Waters Symmetry C 18 (5 μm, 250mm×4.6mm).

[0050] Reagents: acetonitrile, methanol, formic acid (chromatographically pure, other reagents used in the experiment are analytically pure).

[0051] Preparation of drug extracts:

[0052] Take 500 mg of drug powder, use 6.5 ml of mixed solvent (methanol: dichloromethane: water = 4: 2: 0.5, V / V) for ultrasonic extraction for 20 min, and then centrifuge at 12000 r / min for 10 min. The supernatant was filtered through a 0.45 μm filter membrane to obtain a drug extract preparation.

[0053] Prepare blank serum samples:

[0054] Sodium carboxymethylcellulose was made into a 0.5% suspension in pure water for intragastric administration to SD rats (1 ml / kg rat body weight). Afte...

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Abstract

The invention belongs to the pharmaceutical analysis field, relates to a method for determining a medicine active component in serum, especially relates to a method for determining active components of a compound traditional Chinese medicine in serum. The invention employs a high performance liquid chromatography-mass spectrometry method, and extracts of rat serum, blank rat serum and medicament after medication can be analyzed, active component which is capable of entering to blood and performing drug effect in the medicine and is material foundation of the medicine can be determined, and the content of the material foundation in the medicine can be determined on the base. According to the invention, the compound traditional Chinese medicine can be taken as an integral body for researching, the contained compound component group from the medicine in serum can be analyzed, the material foundation of the medicine can be revealed, and the content of medicine active component can be determined, the method for determining the medicine active component in serum has the advantages of good stability and reappearance, strong operationality and the like.

Description

technical field [0001] The invention belongs to the field of drug analysis, and relates to a method for measuring active ingredients of medicine in serum, in particular to a method for measuring active ingredients of compound traditional Chinese medicine in serum. Background technique [0002] With its unique curative effect and relatively small side effects, compound Chinese medicine has been used clinically for thousands of years. However, due to the existence of numerous chemical components, it is quite difficult to study the material basis of the compound Chinese medicine and the mechanism of action of the compound Chinese medicine. Serum medicinal chemistry studies have shown that although traditional Chinese medicine contains many ingredients, only the ingredients absorbed into the blood can produce effects and are considered active ingredients of the drug. Traditional Chinese medicine is mostly administered orally. After oral administration, the drug components are d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/72G01N30/02G01N30/06
Inventor 张卫东丁建弥柳润辉陈忠樑姜鹏徐瑞林张正光孙志男潘芳芳
Owner HEHUANG PHARMA SHANGHAI
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