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Measurement method of honeysuckle and radix scutellariae granule fingerprint spectrum

A fingerprint and measurement method technology, applied in the field of drug analysis, can solve the problems of difficult to accurately evaluate the quality of traditional Chinese medicine products, and the irrelevance of drug efficacy indicators, etc., and achieve the effects of good linear relationship, good repeatability and stability, and monitoring stability

Inactive Publication Date: 2016-08-31
SHANDONG ACAD OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the fingerprints only reflect the chemical information of traditional Chinese medicine, and are not related to the efficacy index, so it is difficult to accurately evaluate the quality of traditional Chinese medicine products, and the efficacy is the ultimate indicator for evaluating the quality of medicines

Method used

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  • Measurement method of honeysuckle and radix scutellariae granule fingerprint spectrum
  • Measurement method of honeysuckle and radix scutellariae granule fingerprint spectrum
  • Measurement method of honeysuckle and radix scutellariae granule fingerprint spectrum

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Embodiment 1

[0031] A silvery yellow granule fingerprint spectrum determination method, is characterized in that, comprises the following steps:

[0032] (1) Preparation of reference solution: Accurately weigh baicalin, baicalein, luteolin, cryptochlorogenic acid, neochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, and Laminin, chrysin, wogonin, and wogonin were dissolved in methanol respectively, and then an equal volume of water was added to prepare 0.1 mg / ml reference solution, and stored at 4°C in the dark.

[0033] (2) Preparation of internal standard solution: Accurately weigh isorhamnetin, add methanol to dissolve, then add an equal volume of water, and set the volume to a concentration of 30 µg·mL -1 internal standard solution.

[0034] Selection of internal standard:

[0035]Paeonol, salvianolic acid B, puerarin, isorhamnetin, hesperidin, kaempferol, quercetin, quercetin and other reference substances were investigated, and isorhamnetin was ...

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Abstract

The invention relates to a quality control method for honeysuckle and radix scutellariae granules, particularly relates to a measurement method of a honeysuckle and radix scutellariae granule fingerprint spectrum, and belongs to the technical field of medicine analysis. The method includes the steps of: 1) preparing a reference substance solution; 2) preparing an internal standard solution; 3) preparing a sample solution; and 4) performing analysis and measurement with high performance liquid chromatography-mass spectrum. Through the HPLC-MS method, chemical components of a common peak of the honeysuckle and radix scutellariae granules are accurately measured. The method is simple and accurate, has high sensitivity and good repeatability, can monitor qualities of raw material medicines, semi-products and finish products more comprehensively, and can also monitor stability of a production process.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for determining the fingerprint of silver yellow granules. Background technique [0002] Yinhuang granule is a compound preparation composed of honeysuckle extract and scutellaria baicalensis extract. It has the functions of clearing heat, detoxifying, and reducing inflammation. It is widely used in the treatment of upper respiratory tract infection, acute tonsillitis, pharyngitis, mumps, ophthalmic diseases, and burn infection. and other diseases. At present, there are 102 enterprises that have obtained the approval number of this variety from the State Food and Drug Administration, and there are many manufacturers. In addition, differences in raw materials and production processes will affect product quality. [0003] Chinese medicine fingerprints use various advanced analysis methods (spectrum, spectrum, chromatography, etc.) to mark the characteristics of Chin...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 高燕吕凌丁晓彦刘青赵渤年
Owner SHANDONG ACAD OF CHINESE MEDICINE
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