Functional pipetting head with double purification functions of ultra-filtration and solid extraction and application of functional pipetting head

Abstract

The invention relates to an experiment device for pretreatment on samples, and in particular to a functional pipetting head with double purification functions of ultra-filtration and solid extraction, and application of the functional pipetting head in pretreatment and quantitative analysis on a biological sample in the medicine analysis process. The functional pipetting head comprises a pipetting head body, a solid-phase extraction material and a hollow fiber membrane, wherein the hollow fiber membrane is arranged at the sharp end of the pipetting head body; an adhesive is uniformly applied at the cross part of the sharp end of the pipetting head and the hollow fiber membrane; an upper end opening and a lower end opening are formed in the hollow fiber membrane; the upper end opening is sealed; the lower end opening is kept in an opened state; the solid-phase extraction material is arranged inside the pipetting head body. The functional pipetting head is simple, convenient and controllable in manufacturing process, flexible in extraction condition adjustment, scientific and reasonable in method, convenient in obtaining materials, and good in application prospect.

Description

technical field [0001] The invention relates to an experimental device for sample pretreatment, in particular to a functionalized pipette tip with double purification functions of ultrafiltration and solid phase extraction, and its use in the pretreatment and quantification of biological samples in the process of drug analysis analyze. Background technique [0002] Solid phase extraction is a kind of sample pretreatment technology. With the rapid development of modern instruments, the requirements for sample pretreatment have also increased accordingly. With its unique advantages, solid phase extraction is different from the traditional liquid-liquid extraction method. Purification plays an increasingly important role. This technology is developed from the combination of liquid-solid extraction and column-liquid chromatography. It can purify samples, transfer target substances from the sample matrix, and enrich and concentrate trace target substances, thereby improving the ...

AI Technical Summary

Problems solved by technology

However, the reproducibility of the preparation of the monolithic column is low, resulting in large differences in results. In addition, because the porosity of the monolithic column is small, it is difficult to absorb liquid, and quantitative absorption of samples cannot be achieved; ② Internal paste type: paste a layer of adsorbent material powder on Inside the pipette tip

However, this method is difficult to quantify the adsorption material, and the quality of the pasted material is limited, so it is difficult to achieve a high adsorption capacity; ③ pointed envelope type; after the adsorption material is wrapped with a microporous filter membrane, it is pasted on the top of the tip of the pipette

However, this method is difficult to manufacture, and factors that affect the adsorption effect, such as the shape and volume of the package, cannot be well controlled, so the reproducibility of the results is low

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