Tandem liquid phase micro-extraction device and method for extracting, purifying and chemically deriving traditional Chinese medicine volatile oil

Abstract

The invention relates to a tandem liquid phase micro-extraction device for extracting, purifying and chemically deriving a traditional Chinese medicine volatile oil. The tandem liquid phase micro-extraction device for extracting, purifying and chemically deriving the traditional Chinese medicine volatile oil comprises an extraction device, a purification device, and a chemical derivatization device; the extraction device comprises a fraction guiding tube, a first heating device and an extraction container; the purification device comprises a separation container, a cooling device, a volatile oil guiding tube, a separation hollow fiber membrane, a first quantitative syringe, and a pressure regulating tube; and the chemical derivatization device comprises a derivatization container, a secondheating device, a derivatization hollow fiber membrane, a sample receiving vial, a second quantitative syringe and an analyte guiding tube. The preparation process of the invention is simple and controllable, the application method is scientific and reasonable, the pre-treatment conditions are flexibly adjusted, the material is convenient to be taken, and the prospect is good.

Description

technical field [0001] The invention relates to methods for quality control and variety identification of traditional Chinese medicines, in particular to a liquid-phase micro-extraction device in series for extraction, purification and chemical derivation of volatile oils of traditional Chinese medicines and its application in chemical composition analysis and fingerprint identification of volatile oils of traditional Chinese medicines. Background technique [0002] Volatile oil is an important component of the chemical components of herbal plants. It has effects in many aspects such as cardiovascular and cerebrovascular systems, central nervous system, respiratory system, gastrointestinal system, antibacterial, anti-inflammatory, anti-tumor, anti-virus and promotion of drug absorption. It is often used to evaluate the quality and identification of Chinese herbal medicines. [0003] At present, the extraction method of volatile oil mainly adopts steam distillation. The boil...

AI Technical Summary

Problems solved by technology

Although this method is widely used, there are also some problems that cannot be ignored: (1) the sampling volume is large

However, the derivatization reaction has the following problems: (1) the amount of sample is large, and generally more than 1g of volatile oil sample needs to be taken; (2) the reaction operation is cumbersome, and the derivatization reaction often requires multiple steps of centrifugation, heating, and filtration. (3) The amount of organic solvent used is large, because the derivatization reaction often has the problem of excessive derivatization reagents, and a large amount of organic solvents are needed to remove the excess derivatization reagents, so as not to cause detection difficulties

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