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Sulphated compound microalgae polysaccharide and preparation method and application thereof

A technology of microalgae sulphate and sulphate, which is applied in drug combinations, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve problems such as anti-tumor activity that has not been found, and achieve good anti-tumor, growth inhibition, and anti-tumor effects. The effect of in vitro proliferation

Active Publication Date: 2018-01-12
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] Through the search, no research has been found on the combination of spirulina and chlorella for anti-tumor activity

Method used

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  • Sulphated compound microalgae polysaccharide and preparation method and application thereof
  • Sulphated compound microalgae polysaccharide and preparation method and application thereof
  • Sulphated compound microalgae polysaccharide and preparation method and application thereof

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Embodiment Construction

[0050] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0051] 1. Preparation of chlorella polysaccharide (CSP) and spirulina polysaccharide (PSP)

[0052] (1) Wall breaking of Spirulina or Chlorella cells: Centrifuge the Spirulina or Chlorella liquid that has grown to the plateau stage, weigh 10g of algae powder, add 90mL of distilled water, and freeze the algae liquid at -20°C overnight Agglomerate, then heat in a water bath for 8 hours to thaw and break the wall, repeat the above process 3 times, centrifuge at 4500r / min for 10 minutes, take the supernatant, shake the algae mud, add distilled water and then ultrasonically break the wall: 600-800W, 1 minute each time, 10 minutes in total After completion, centrifuge at 4500r / min for 10min to take the sup...

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Abstract

The invention relates to sulphated compound microalgae polysaccharide. The sulphated compound microalgae polysaccharide is prepared by mixing sulphated chlorella polysaccharide and sulphated spirulinaon the base of the ratio of 1 to 16-16 to 1. The sulphated compound microalgae polysaccharide is capable of effectively inhibiting in-vitro proliferation of tumor cells, and is high in water solubility, so that the sulphated compound microalgae polysaccharide can play a role in vitro and is capable of inhibiting the growth of tumor.

Description

technical field [0001] The invention belongs to the medical application field of marine organisms, and relates to spirulina and chlorella, in particular to a microalgae sulfated composite polysaccharide, a preparation method and application thereof. Background technique [0002] The growth conditions and environmental characteristics of microalgae determine that the intracellular polysaccharides of microalgae have some structures and functions that are different from other biological polysaccharides. The application potential of such polysaccharides in the field of medicine and medicine has attracted more and more people's research interest in them. However, a large number of studies in recent years have found that microalgal polysaccharides from different sources have different biological activities, and the biological activity of a single polysaccharide cannot comprehensively improve the immunity of the human body. [0003] Spirulina (Spirulina platensis) is a class of low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 宋东辉
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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